Cartridge-type measurement system for local field potential to develop intelligent robot with cerebral organoid
Abstract Cerebral organoid, that is cultured from iPS cells, is known as a counterpart of the human brain. Therefore, we expect that cerebral organoids can be used as processing units made of biological materials for bio-machine hybrid systems. Related works have demonstrated the potential of cerebr...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2025-06-01
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| Series: | ROBOMECH Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40648-025-00305-6 |
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| Summary: | Abstract Cerebral organoid, that is cultured from iPS cells, is known as a counterpart of the human brain. Therefore, we expect that cerebral organoids can be used as processing units made of biological materials for bio-machine hybrid systems. Related works have demonstrated the potential of cerebral organoids as processing units. However, no application of cerebral organoids as learning intelligent systems has been demonstrated. We have previously developed a prototype robot in which cerebral organoids were co-cultured with muscle cells. As a next step, it is necessary to clarify how cerebral organoids can be used as living CPUs for novel intelligent systems in robotics as well. In this study, we consider how to easily use cerebral organoids as the CPU of a robot. Therefore, we consider a measurement system that can measure the local field potential of cerebral organoids while simultaneously observing them with an optical microscope. Furthermore, we consider not only measurement but also applying electrical stimuli to the cerebral organoids from the outside. The measurement system should be designed for easy mounting of cerebral organoids onto robotic systems. The shape of the measurement chamber and electrodes should be simple enough to allow seamless connection to traditional instruments and control systems. These design aspects enhance the system's usability. Based on the above discussion, the purpose of this study is to develop a cartridge-type measurement system for cerebral organoids that will facilitate their application as intelligent systems. This study developed a cartridge-type measurement system for cerebral organoids that will facilitate their application as intelligent systems. This paper described the specifications of the cartridge-type measurement system developed. We explained the method for culturing cerebral organoids. We conducted measuring the local field potential of cerebral organoids using the proposed cartridge-type measurement system. We demonstrated the successful measurement of local field potentials in multiple individual cerebral organoids. We discussed the perspective of learning by cerebral organoids and possible applications of the proposed system. In the future, we expect that our research will lead to novel regenerative medicine with cyborg technology. |
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| ISSN: | 2197-4225 |