Persistent Penumbra in a Rabbit Stroke Model: Incidence and Histologic Characteristics
Duration and extent of penumbra determine the window and brain volume in which interventions may save injured tissue after stroke. Understanding the penumbra in animals is necessary in order to design models that translate to effective clinical therapies. New Zealand white rabbits were embolized wit...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | Stroke Research and Treatment |
| Online Access: | http://dx.doi.org/10.4061/2011/764830 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850173186005532672 |
|---|---|
| author | Leah J. Hennings Rene Flores Paula K. Roberson Aliza Brown John Lowery Michael Borrelli William C. Culp |
| author_facet | Leah J. Hennings Rene Flores Paula K. Roberson Aliza Brown John Lowery Michael Borrelli William C. Culp |
| author_sort | Leah J. Hennings |
| collection | DOAJ |
| description | Duration and extent of penumbra determine the window and brain volume in which interventions may save injured tissue after stroke. Understanding the penumbra in animals is necessary in order to design models that translate to effective clinical therapies. New Zealand white rabbits were embolized with aged autologous clot (n=23) or insoluble microspheres (n=21). To examine effects of treatment on penumbra, sphere-stroked animals were treated with 3 μm microbubbles plus ultrasound (n=19). Rabbits were euthanized at 4 or 24 hr. Infarct volume was measured following triphenyltetrazolium chloride (TTC) staining of brain sections. Penumbra was visualized using immunostaining of pimonidazole injected fifteen minutes prior to euthanasia. Potentially reversible penumbra was present in 14.3% stroked rabbits at 4 hours and 15.7% at 24 hours after embolic stroke and represented up to 35% of total lost tissue. Intervention at up to 24 hours may benefit a significant patient population. |
| format | Article |
| id | doaj-art-d10a417e60954c4e8781840852ea2791 |
| institution | OA Journals |
| issn | 2042-0056 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stroke Research and Treatment |
| spelling | doaj-art-d10a417e60954c4e8781840852ea27912025-08-20T02:19:54ZengWileyStroke Research and Treatment2042-00562011-01-01201110.4061/2011/764830764830Persistent Penumbra in a Rabbit Stroke Model: Incidence and Histologic CharacteristicsLeah J. Hennings0Rene Flores1Paula K. Roberson2Aliza Brown3John Lowery4Michael Borrelli5William C. Culp6Department of Pathology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USADepartment of Radiology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USADepartment of Biostatistics, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USADepartment of Radiology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USADivision of Laboratory Animal Medicine, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USADepartment of Radiology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USADepartment of Radiology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USADuration and extent of penumbra determine the window and brain volume in which interventions may save injured tissue after stroke. Understanding the penumbra in animals is necessary in order to design models that translate to effective clinical therapies. New Zealand white rabbits were embolized with aged autologous clot (n=23) or insoluble microspheres (n=21). To examine effects of treatment on penumbra, sphere-stroked animals were treated with 3 μm microbubbles plus ultrasound (n=19). Rabbits were euthanized at 4 or 24 hr. Infarct volume was measured following triphenyltetrazolium chloride (TTC) staining of brain sections. Penumbra was visualized using immunostaining of pimonidazole injected fifteen minutes prior to euthanasia. Potentially reversible penumbra was present in 14.3% stroked rabbits at 4 hours and 15.7% at 24 hours after embolic stroke and represented up to 35% of total lost tissue. Intervention at up to 24 hours may benefit a significant patient population.http://dx.doi.org/10.4061/2011/764830 |
| spellingShingle | Leah J. Hennings Rene Flores Paula K. Roberson Aliza Brown John Lowery Michael Borrelli William C. Culp Persistent Penumbra in a Rabbit Stroke Model: Incidence and Histologic Characteristics Stroke Research and Treatment |
| title | Persistent Penumbra in a Rabbit Stroke Model: Incidence and Histologic Characteristics |
| title_full | Persistent Penumbra in a Rabbit Stroke Model: Incidence and Histologic Characteristics |
| title_fullStr | Persistent Penumbra in a Rabbit Stroke Model: Incidence and Histologic Characteristics |
| title_full_unstemmed | Persistent Penumbra in a Rabbit Stroke Model: Incidence and Histologic Characteristics |
| title_short | Persistent Penumbra in a Rabbit Stroke Model: Incidence and Histologic Characteristics |
| title_sort | persistent penumbra in a rabbit stroke model incidence and histologic characteristics |
| url | http://dx.doi.org/10.4061/2011/764830 |
| work_keys_str_mv | AT leahjhennings persistentpenumbrainarabbitstrokemodelincidenceandhistologiccharacteristics AT reneflores persistentpenumbrainarabbitstrokemodelincidenceandhistologiccharacteristics AT paulakroberson persistentpenumbrainarabbitstrokemodelincidenceandhistologiccharacteristics AT alizabrown persistentpenumbrainarabbitstrokemodelincidenceandhistologiccharacteristics AT johnlowery persistentpenumbrainarabbitstrokemodelincidenceandhistologiccharacteristics AT michaelborrelli persistentpenumbrainarabbitstrokemodelincidenceandhistologiccharacteristics AT williamcculp persistentpenumbrainarabbitstrokemodelincidenceandhistologiccharacteristics |