MyosinVIIa interacts with Twinfilin-2 at the tips of mechanosensory stereocilia in the inner ear.
In vertebrates hearing is dependent upon the microvilli-like mechanosensory stereocilia and their length gradation. The staircase-like organization of the stereocilia bundle is dynamically maintained by variable actin turnover rates. Two unconventional myosins were previously implicated in stereocil...
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Public Library of Science (PLoS)
2009-09-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0007097&type=printable |
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| author | Agnieszka K Rzadzinska Elisa M Nevalainen Haydn M Prosser Pekka Lappalainen Karen P Steel |
| author_facet | Agnieszka K Rzadzinska Elisa M Nevalainen Haydn M Prosser Pekka Lappalainen Karen P Steel |
| author_sort | Agnieszka K Rzadzinska |
| collection | DOAJ |
| description | In vertebrates hearing is dependent upon the microvilli-like mechanosensory stereocilia and their length gradation. The staircase-like organization of the stereocilia bundle is dynamically maintained by variable actin turnover rates. Two unconventional myosins were previously implicated in stereocilia length regulation but the mechanisms of their action remain unknown. MyosinXVa is expressed in stereocilia tips at levels proportional to stereocilia length and its absence produces staircase-like bundles of very short stereocilia. MyosinVIIa localizes to the tips of the shorter stereocilia within bundles, and when absent, the stereocilia are abnormally long. We show here that myosinVIIa interacts with twinfilin-2, an actin binding protein, which inhibits actin polymerization at the barbed end of the filament, and that twinfilin localization in stereocilia overlaps with myosinVIIa. Exogenous expression of myosinVIIa in fibroblasts results in a reduced number of filopodia and promotes accumulation of twinfilin-2 at the filopodia tips. We hypothesize that the newly described interaction between myosinVIIa and twinfilin-2 is responsible for the establishment and maintenance of slower rates of actin turnover in shorter stereocilia, and that interplay between complexes of myosinVIIa/twinfilin-2 and myosinXVa/whirlin is responsible for stereocilia length gradation within the bundle staircase. |
| format | Article |
| id | doaj-art-d0faaa97b07c49db9f744e280a68236b |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2009-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
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| spelling | doaj-art-d0faaa97b07c49db9f744e280a68236b2025-08-20T03:07:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-09-0149e709710.1371/journal.pone.0007097MyosinVIIa interacts with Twinfilin-2 at the tips of mechanosensory stereocilia in the inner ear.Agnieszka K RzadzinskaElisa M NevalainenHaydn M ProsserPekka LappalainenKaren P SteelIn vertebrates hearing is dependent upon the microvilli-like mechanosensory stereocilia and their length gradation. The staircase-like organization of the stereocilia bundle is dynamically maintained by variable actin turnover rates. Two unconventional myosins were previously implicated in stereocilia length regulation but the mechanisms of their action remain unknown. MyosinXVa is expressed in stereocilia tips at levels proportional to stereocilia length and its absence produces staircase-like bundles of very short stereocilia. MyosinVIIa localizes to the tips of the shorter stereocilia within bundles, and when absent, the stereocilia are abnormally long. We show here that myosinVIIa interacts with twinfilin-2, an actin binding protein, which inhibits actin polymerization at the barbed end of the filament, and that twinfilin localization in stereocilia overlaps with myosinVIIa. Exogenous expression of myosinVIIa in fibroblasts results in a reduced number of filopodia and promotes accumulation of twinfilin-2 at the filopodia tips. We hypothesize that the newly described interaction between myosinVIIa and twinfilin-2 is responsible for the establishment and maintenance of slower rates of actin turnover in shorter stereocilia, and that interplay between complexes of myosinVIIa/twinfilin-2 and myosinXVa/whirlin is responsible for stereocilia length gradation within the bundle staircase.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0007097&type=printable |
| spellingShingle | Agnieszka K Rzadzinska Elisa M Nevalainen Haydn M Prosser Pekka Lappalainen Karen P Steel MyosinVIIa interacts with Twinfilin-2 at the tips of mechanosensory stereocilia in the inner ear. PLoS ONE |
| title | MyosinVIIa interacts with Twinfilin-2 at the tips of mechanosensory stereocilia in the inner ear. |
| title_full | MyosinVIIa interacts with Twinfilin-2 at the tips of mechanosensory stereocilia in the inner ear. |
| title_fullStr | MyosinVIIa interacts with Twinfilin-2 at the tips of mechanosensory stereocilia in the inner ear. |
| title_full_unstemmed | MyosinVIIa interacts with Twinfilin-2 at the tips of mechanosensory stereocilia in the inner ear. |
| title_short | MyosinVIIa interacts with Twinfilin-2 at the tips of mechanosensory stereocilia in the inner ear. |
| title_sort | myosinviia interacts with twinfilin 2 at the tips of mechanosensory stereocilia in the inner ear |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0007097&type=printable |
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