Soybean <i>GmSNF4</i> Confers Salt–Alkali Stress Tolerance in Transgenic Plants
In order to mitigate the reduction in soybean yield caused by soil salinization, a soybean gene, <i>GmSNF4</i>, which promotes plant tolerance to salt–alkali stress, was identified in this study. The STRING database was used to predict the interaction between GmSNF4 and GmPKS4. The <i...
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2025-07-01
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| author | Nan Ye Jia-Shen Bian Bai-Hui Zhou Ling-Tao Yong Ting Yang Nan Wang Yuan-Yuan Dong Wei-Can Liu Fa-Wei Wang Hai-Yan Lv Xiao-Wei Li |
| author_facet | Nan Ye Jia-Shen Bian Bai-Hui Zhou Ling-Tao Yong Ting Yang Nan Wang Yuan-Yuan Dong Wei-Can Liu Fa-Wei Wang Hai-Yan Lv Xiao-Wei Li |
| author_sort | Nan Ye |
| collection | DOAJ |
| description | In order to mitigate the reduction in soybean yield caused by soil salinization, a soybean gene, <i>GmSNF4</i>, which promotes plant tolerance to salt–alkali stress, was identified in this study. The STRING database was used to predict the interaction between GmSNF4 and GmPKS4. The <i>GmPKS4</i> gene was experimentally shown to be involved in salt–alkali stress tolerance. Firstly, the yeast two-hybrid technique and bimolecular fluorescence complementation (BiFC) technique were used to confirm the interaction between GmSNF4 and GmPKS4: the AMPK-CBM-CBS1 conserved domain was thereby determined to be the region of the GmSNF4 protein involved in the interaction. Secondly, the <i>GmSNF4</i> gene was induced by salt–alkali stress according to qRT-PCR analysis, and the GmSNF4 protein was localized in the nucleus and cytoplasm. Finally, analysis of <i>GmSNF4</i>’s role in resistance to salt–alkali stress in transgenic soybean plants showed that transgenic lines had better phenotypic, physiological, and stress-related gene expression than non-transgenic soybeans. Thus, <i>GmSNF4</i> may play a significant role in plant salt–alkali stress tolerance. |
| format | Article |
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| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-d0f8b69c596a4a7c92bf988e80be254e2025-08-20T03:56:47ZengMDPI AGPlants2223-77472025-07-011414221810.3390/plants14142218Soybean <i>GmSNF4</i> Confers Salt–Alkali Stress Tolerance in Transgenic PlantsNan Ye0Jia-Shen Bian1Bai-Hui Zhou2Ling-Tao Yong3Ting Yang4Nan Wang5Yuan-Yuan Dong6Wei-Can Liu7Fa-Wei Wang8Hai-Yan Lv9Xiao-Wei Li10College of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaIn order to mitigate the reduction in soybean yield caused by soil salinization, a soybean gene, <i>GmSNF4</i>, which promotes plant tolerance to salt–alkali stress, was identified in this study. The STRING database was used to predict the interaction between GmSNF4 and GmPKS4. The <i>GmPKS4</i> gene was experimentally shown to be involved in salt–alkali stress tolerance. Firstly, the yeast two-hybrid technique and bimolecular fluorescence complementation (BiFC) technique were used to confirm the interaction between GmSNF4 and GmPKS4: the AMPK-CBM-CBS1 conserved domain was thereby determined to be the region of the GmSNF4 protein involved in the interaction. Secondly, the <i>GmSNF4</i> gene was induced by salt–alkali stress according to qRT-PCR analysis, and the GmSNF4 protein was localized in the nucleus and cytoplasm. Finally, analysis of <i>GmSNF4</i>’s role in resistance to salt–alkali stress in transgenic soybean plants showed that transgenic lines had better phenotypic, physiological, and stress-related gene expression than non-transgenic soybeans. Thus, <i>GmSNF4</i> may play a significant role in plant salt–alkali stress tolerance.https://www.mdpi.com/2223-7747/14/14/2218<i>GmSNF4</i>soybeansalt–alkali stresstransgenic plants |
| spellingShingle | Nan Ye Jia-Shen Bian Bai-Hui Zhou Ling-Tao Yong Ting Yang Nan Wang Yuan-Yuan Dong Wei-Can Liu Fa-Wei Wang Hai-Yan Lv Xiao-Wei Li Soybean <i>GmSNF4</i> Confers Salt–Alkali Stress Tolerance in Transgenic Plants Plants <i>GmSNF4</i> soybean salt–alkali stress transgenic plants |
| title | Soybean <i>GmSNF4</i> Confers Salt–Alkali Stress Tolerance in Transgenic Plants |
| title_full | Soybean <i>GmSNF4</i> Confers Salt–Alkali Stress Tolerance in Transgenic Plants |
| title_fullStr | Soybean <i>GmSNF4</i> Confers Salt–Alkali Stress Tolerance in Transgenic Plants |
| title_full_unstemmed | Soybean <i>GmSNF4</i> Confers Salt–Alkali Stress Tolerance in Transgenic Plants |
| title_short | Soybean <i>GmSNF4</i> Confers Salt–Alkali Stress Tolerance in Transgenic Plants |
| title_sort | soybean i gmsnf4 i confers salt alkali stress tolerance in transgenic plants |
| topic | <i>GmSNF4</i> soybean salt–alkali stress transgenic plants |
| url | https://www.mdpi.com/2223-7747/14/14/2218 |
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