Multifunctional micro/nano-textured titanium with bactericidal, osteogenic, angiogenic and anti-inflammatory properties: Insights from in vitro and in vivo studies
Titanium (Ti) is widely used as an implantable material for bone repair in orthopedics and dentistry. However, Ti implants are vulnerable to bacterial infections, which can compromise patient recovery and lead to implant failure. While a controlled inflammatory response promotes bone regeneration, c...
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Elsevier
2025-06-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425002698 |
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| author | Théo Ziegelmeyer Karolinne Martins de Sousa Tzu-Ying Liao Rodolphe Lartizien Alexandra Delay Julien Vollaire Véronique Josserand Denver Linklater Phuc H. Le Jean-Luc Coll Georges Bettega Elena P. Ivanova Véronique Martel-Frachet |
| author_facet | Théo Ziegelmeyer Karolinne Martins de Sousa Tzu-Ying Liao Rodolphe Lartizien Alexandra Delay Julien Vollaire Véronique Josserand Denver Linklater Phuc H. Le Jean-Luc Coll Georges Bettega Elena P. Ivanova Véronique Martel-Frachet |
| author_sort | Théo Ziegelmeyer |
| collection | DOAJ |
| description | Titanium (Ti) is widely used as an implantable material for bone repair in orthopedics and dentistry. However, Ti implants are vulnerable to bacterial infections, which can compromise patient recovery and lead to implant failure. While a controlled inflammatory response promotes bone regeneration, chronic inflammation caused by infections can lead to implant failure. Bone repair is a complex process in which inflammation, angiogenesis and osteogenesis are tightly interconnected, requiring cooperation between mesenchymal stem cells (MSC), macrophages and endothelial cells. Here, we fabricated bio-inspired Ti implants with either microstructured (Micro Ti) or nanostructured (Nano Ti) surface textures that exhibit robust mechano-bactericidal properties. In vitro, both textured surfaces improved blood coagulation and osteogenic marker expression compared to smooth Ti surfaces. Additionally, Nano Ti promoted macrophage polarization towards the M2 phenotype and enhanced the paracrine effects of MSCs on angiogenesis, key processes in tissue regeneration. In vivo kinetic analysis of bone reconstruction in a rat calvarial model showed that Nano Ti improved osseointegration, as evidenced by increased bone volume, mineral density, and bone-implant contact. Notably, the Micro Ti surface showed no significant differences from the control implants. These findings highlight the potential of mechano-bactericidal surface nanopatterns to simultaneously prevent infections and enhance osseointegration by modulating protein adsorption, inflammation, angiogenesis and osteogenesis. This study provides new insights into the development of bifunctional Ti implants, offering new perspectives for the next generation of implantable bone-related biomaterials. |
| format | Article |
| id | doaj-art-d0efeeb6d4ec4e17aa16846483f25f62 |
| institution | DOAJ |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-d0efeeb6d4ec4e17aa16846483f25f622025-08-20T02:49:25ZengElsevierMaterials Today Bio2590-00642025-06-013210171010.1016/j.mtbio.2025.101710Multifunctional micro/nano-textured titanium with bactericidal, osteogenic, angiogenic and anti-inflammatory properties: Insights from in vitro and in vivo studiesThéo Ziegelmeyer0Karolinne Martins de Sousa1Tzu-Ying Liao2Rodolphe Lartizien3Alexandra Delay4Julien Vollaire5Véronique Josserand6Denver Linklater7Phuc H. Le8Jean-Luc Coll9Georges Bettega10Elena P. Ivanova11Véronique Martel-Frachet12University Grenoble Alpes, INSERM U1209, CNRS UMR5309, Institute for Advanced Biosciences, F38000, Grenoble, FranceSchool of Science, RMIT University, Melbourne, VIC, 3000, AustraliaSchool of Science, RMIT University, Melbourne, VIC, 3000, AustraliaService de Chirurgie Maxillo-Faciale, Centre Hospitalier Annecy Genevois, 1 Avenue de L'hôpital, Epagny Metz-Tessy, F-74370, FranceUniversity Grenoble Alpes, INSERM U1209, CNRS UMR5309, Institute for Advanced Biosciences, F38000, Grenoble, FranceUniversity Grenoble Alpes, INSERM U1209, CNRS UMR5309, Institute for Advanced Biosciences, F38000, Grenoble, France; University Grenoble Alpes, INSERM U1209, CNRS UMR5309, Optimal Platform, Institute for Advanced Biosciences, 38000, Grenoble, FranceUniversity Grenoble Alpes, INSERM U1209, CNRS UMR5309, Institute for Advanced Biosciences, F38000, Grenoble, France; University Grenoble Alpes, INSERM U1209, CNRS UMR5309, Optimal Platform, Institute for Advanced Biosciences, 38000, Grenoble, FranceDepartment of Biomedical Engineering, The Graeme Clark Institute, The University of Melbourne, Parkville, VIC, 3010, AustraliaSchool of Science, RMIT University, Melbourne, VIC, 3000, AustraliaUniversity Grenoble Alpes, INSERM U1209, CNRS UMR5309, Institute for Advanced Biosciences, F38000, Grenoble, FranceUniversity Grenoble Alpes, INSERM U1209, CNRS UMR5309, Institute for Advanced Biosciences, F38000, Grenoble, France; Service de Chirurgie Maxillo-Faciale, Centre Hospitalier Annecy Genevois, 1 Avenue de L'hôpital, Epagny Metz-Tessy, F-74370, FranceSchool of Science, RMIT University, Melbourne, VIC, 3000, Australia; Corresponding author.University Grenoble Alpes, INSERM U1209, CNRS UMR5309, Institute for Advanced Biosciences, F38000, Grenoble, France; University PSL Research, EPHE, F75014, Paris, France; Corresponding author. University Grenoble Alpes, INSERM U1209, CNRS UMR5309, Institute for Advanced Biosciences, F38000, Grenoble, France.Titanium (Ti) is widely used as an implantable material for bone repair in orthopedics and dentistry. However, Ti implants are vulnerable to bacterial infections, which can compromise patient recovery and lead to implant failure. While a controlled inflammatory response promotes bone regeneration, chronic inflammation caused by infections can lead to implant failure. Bone repair is a complex process in which inflammation, angiogenesis and osteogenesis are tightly interconnected, requiring cooperation between mesenchymal stem cells (MSC), macrophages and endothelial cells. Here, we fabricated bio-inspired Ti implants with either microstructured (Micro Ti) or nanostructured (Nano Ti) surface textures that exhibit robust mechano-bactericidal properties. In vitro, both textured surfaces improved blood coagulation and osteogenic marker expression compared to smooth Ti surfaces. Additionally, Nano Ti promoted macrophage polarization towards the M2 phenotype and enhanced the paracrine effects of MSCs on angiogenesis, key processes in tissue regeneration. In vivo kinetic analysis of bone reconstruction in a rat calvarial model showed that Nano Ti improved osseointegration, as evidenced by increased bone volume, mineral density, and bone-implant contact. Notably, the Micro Ti surface showed no significant differences from the control implants. These findings highlight the potential of mechano-bactericidal surface nanopatterns to simultaneously prevent infections and enhance osseointegration by modulating protein adsorption, inflammation, angiogenesis and osteogenesis. This study provides new insights into the development of bifunctional Ti implants, offering new perspectives for the next generation of implantable bone-related biomaterials.http://www.sciencedirect.com/science/article/pii/S2590006425002698Bioinspired mechano-bactericidal surfacesBone reconstructionTitanium implantsOsseointegrationSurface topography |
| spellingShingle | Théo Ziegelmeyer Karolinne Martins de Sousa Tzu-Ying Liao Rodolphe Lartizien Alexandra Delay Julien Vollaire Véronique Josserand Denver Linklater Phuc H. Le Jean-Luc Coll Georges Bettega Elena P. Ivanova Véronique Martel-Frachet Multifunctional micro/nano-textured titanium with bactericidal, osteogenic, angiogenic and anti-inflammatory properties: Insights from in vitro and in vivo studies Materials Today Bio Bioinspired mechano-bactericidal surfaces Bone reconstruction Titanium implants Osseointegration Surface topography |
| title | Multifunctional micro/nano-textured titanium with bactericidal, osteogenic, angiogenic and anti-inflammatory properties: Insights from in vitro and in vivo studies |
| title_full | Multifunctional micro/nano-textured titanium with bactericidal, osteogenic, angiogenic and anti-inflammatory properties: Insights from in vitro and in vivo studies |
| title_fullStr | Multifunctional micro/nano-textured titanium with bactericidal, osteogenic, angiogenic and anti-inflammatory properties: Insights from in vitro and in vivo studies |
| title_full_unstemmed | Multifunctional micro/nano-textured titanium with bactericidal, osteogenic, angiogenic and anti-inflammatory properties: Insights from in vitro and in vivo studies |
| title_short | Multifunctional micro/nano-textured titanium with bactericidal, osteogenic, angiogenic and anti-inflammatory properties: Insights from in vitro and in vivo studies |
| title_sort | multifunctional micro nano textured titanium with bactericidal osteogenic angiogenic and anti inflammatory properties insights from in vitro and in vivo studies |
| topic | Bioinspired mechano-bactericidal surfaces Bone reconstruction Titanium implants Osseointegration Surface topography |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425002698 |
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