Molecular characterization of indigenous human adenovirus (HAdV) isolate from healthy infant stool sample and screening of its antibodies in archival serum samples in Türkiye.
Human adenoviruses (HAdV) are significant etiological agents of infections affecting the respiratory, gastrointestinal, urinary and ocular systems, particularly in adults, infants, and immunocompromised individuals. This study presents the molecular identification of a local HAdV strain for the firs...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0328556 |
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| author | Zafer Yazici Huseyin Baskin Seda Gozel Hanne Nur Kurucay Cuneyt Tamer Hamza Kadi Emre Ozan Bahadir Muftuoglu Vahide Bayrakal Harun Albayrak Semra Okur-Gumusova Ahmed Eisa Elhag |
| author_facet | Zafer Yazici Huseyin Baskin Seda Gozel Hanne Nur Kurucay Cuneyt Tamer Hamza Kadi Emre Ozan Bahadir Muftuoglu Vahide Bayrakal Harun Albayrak Semra Okur-Gumusova Ahmed Eisa Elhag |
| author_sort | Zafer Yazici |
| collection | DOAJ |
| description | Human adenoviruses (HAdV) are significant etiological agents of infections affecting the respiratory, gastrointestinal, urinary and ocular systems, particularly in adults, infants, and immunocompromised individuals. This study presents the molecular identification of a local HAdV strain for the first time from the stool of a healthy infant in Türkiye, isolated in 2003 and stored for two decades in liquid nitrogen. Molecular characterization of this strain was performed, identifying it as HAdV-C6. Phylogenetic analysis revealed high nucleotide identity (97%) with global strains from Russia, China, Japan, and the USA. A serum neutralization test was conducted to determine the current circulation of this strain, indicating a 9.5% seropositivity rate in archival serum samples collected for the West Nile virus surveillance project. This study provides insights into the persistence and molecular epidemiology of HAdV strains circulating in Türkiye, highlighting the need for continuous surveillance and whole-genome sequencing to assess potential recombination events and genetic variations. |
| format | Article |
| id | doaj-art-d0e90358f721415c80f03b97073e61fe |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-d0e90358f721415c80f03b97073e61fe2025-08-20T02:50:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01207e032855610.1371/journal.pone.0328556Molecular characterization of indigenous human adenovirus (HAdV) isolate from healthy infant stool sample and screening of its antibodies in archival serum samples in Türkiye.Zafer YaziciHuseyin BaskinSeda GozelHanne Nur KurucayCuneyt TamerHamza KadiEmre OzanBahadir MuftuogluVahide BayrakalHarun AlbayrakSemra Okur-GumusovaAhmed Eisa ElhagHuman adenoviruses (HAdV) are significant etiological agents of infections affecting the respiratory, gastrointestinal, urinary and ocular systems, particularly in adults, infants, and immunocompromised individuals. This study presents the molecular identification of a local HAdV strain for the first time from the stool of a healthy infant in Türkiye, isolated in 2003 and stored for two decades in liquid nitrogen. Molecular characterization of this strain was performed, identifying it as HAdV-C6. Phylogenetic analysis revealed high nucleotide identity (97%) with global strains from Russia, China, Japan, and the USA. A serum neutralization test was conducted to determine the current circulation of this strain, indicating a 9.5% seropositivity rate in archival serum samples collected for the West Nile virus surveillance project. This study provides insights into the persistence and molecular epidemiology of HAdV strains circulating in Türkiye, highlighting the need for continuous surveillance and whole-genome sequencing to assess potential recombination events and genetic variations.https://doi.org/10.1371/journal.pone.0328556 |
| spellingShingle | Zafer Yazici Huseyin Baskin Seda Gozel Hanne Nur Kurucay Cuneyt Tamer Hamza Kadi Emre Ozan Bahadir Muftuoglu Vahide Bayrakal Harun Albayrak Semra Okur-Gumusova Ahmed Eisa Elhag Molecular characterization of indigenous human adenovirus (HAdV) isolate from healthy infant stool sample and screening of its antibodies in archival serum samples in Türkiye. PLoS ONE |
| title | Molecular characterization of indigenous human adenovirus (HAdV) isolate from healthy infant stool sample and screening of its antibodies in archival serum samples in Türkiye. |
| title_full | Molecular characterization of indigenous human adenovirus (HAdV) isolate from healthy infant stool sample and screening of its antibodies in archival serum samples in Türkiye. |
| title_fullStr | Molecular characterization of indigenous human adenovirus (HAdV) isolate from healthy infant stool sample and screening of its antibodies in archival serum samples in Türkiye. |
| title_full_unstemmed | Molecular characterization of indigenous human adenovirus (HAdV) isolate from healthy infant stool sample and screening of its antibodies in archival serum samples in Türkiye. |
| title_short | Molecular characterization of indigenous human adenovirus (HAdV) isolate from healthy infant stool sample and screening of its antibodies in archival serum samples in Türkiye. |
| title_sort | molecular characterization of indigenous human adenovirus hadv isolate from healthy infant stool sample and screening of its antibodies in archival serum samples in turkiye |
| url | https://doi.org/10.1371/journal.pone.0328556 |
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