Genome-Wide Identification and Functional Analysis of <i>AP2/ERF</i> Gene Family in <i>Passiflora edulis</i> Sims

The Apetala2/Ethylene Responsive Factor (AP2/ERF) family represents a critical group of transcription factors in plants, recognized for their roles in growth, development, fruit ripening, and postharvest processes. This study aimed to identify and characterize the <i>AP2/ERF</i> gene fam...

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Main Authors: Lanjun Luo, Liping Zhang, Ronghao Gu, Shihao Ni, Jingyao Yu, Yachao Gao, Chuanying Fang
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/5/645
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author Lanjun Luo
Liping Zhang
Ronghao Gu
Shihao Ni
Jingyao Yu
Yachao Gao
Chuanying Fang
author_facet Lanjun Luo
Liping Zhang
Ronghao Gu
Shihao Ni
Jingyao Yu
Yachao Gao
Chuanying Fang
author_sort Lanjun Luo
collection DOAJ
description The Apetala2/Ethylene Responsive Factor (AP2/ERF) family represents a critical group of transcription factors in plants, recognized for their roles in growth, development, fruit ripening, and postharvest processes. This study aimed to identify and characterize the <i>AP2/ERF</i> gene family in passion fruit (<i>Passiflora edulis</i> Sims) and investigate their potential roles in flavor enhancement. A total of 91 <i>PeAP2/ERF</i> genes were identified and classified into five subfamilies. Chromosome localization and collinearity analysis demonstrated their distribution across all nine chromosomes of passion fruit, with tandem duplication events identified as a key driver of family expansion. Exon–intron configurations and motif compositions were highly conserved among <i>PeAP2/ERF</i> genes. Promoter cis-acting element analysis indicated potential regulation by environmental signals, including abiotic and biotic stresses, as well as hormonal cues. Postharvest storage induced the expression of 59 <i>PeAP2/ERF</i> genes over time. Notably, <i>PeAP2-10</i> was found to enhance the expression of <i>PeSTP6</i>, a gene associated with sugar transport, suggesting its potential influence on the flavor profile of passion fruit. These findings provide valuable insights into the functional roles of <i>PeAP2</i>/<i>ERF</i> genes in passion fruit, highlighting their significance in postharvest management and flavor quality enhancement strategies.
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spelling doaj-art-d0e568c61d4d4dac8c3a41902ab108af2025-08-20T02:59:00ZengMDPI AGPlants2223-77472025-02-0114564510.3390/plants14050645Genome-Wide Identification and Functional Analysis of <i>AP2/ERF</i> Gene Family in <i>Passiflora edulis</i> SimsLanjun Luo0Liping Zhang1Ronghao Gu2Shihao Ni3Jingyao Yu4Yachao Gao5Chuanying Fang6School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572025, ChinaSchool of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572025, ChinaThe Apetala2/Ethylene Responsive Factor (AP2/ERF) family represents a critical group of transcription factors in plants, recognized for their roles in growth, development, fruit ripening, and postharvest processes. This study aimed to identify and characterize the <i>AP2/ERF</i> gene family in passion fruit (<i>Passiflora edulis</i> Sims) and investigate their potential roles in flavor enhancement. A total of 91 <i>PeAP2/ERF</i> genes were identified and classified into five subfamilies. Chromosome localization and collinearity analysis demonstrated their distribution across all nine chromosomes of passion fruit, with tandem duplication events identified as a key driver of family expansion. Exon–intron configurations and motif compositions were highly conserved among <i>PeAP2/ERF</i> genes. Promoter cis-acting element analysis indicated potential regulation by environmental signals, including abiotic and biotic stresses, as well as hormonal cues. Postharvest storage induced the expression of 59 <i>PeAP2/ERF</i> genes over time. Notably, <i>PeAP2-10</i> was found to enhance the expression of <i>PeSTP6</i>, a gene associated with sugar transport, suggesting its potential influence on the flavor profile of passion fruit. These findings provide valuable insights into the functional roles of <i>PeAP2</i>/<i>ERF</i> genes in passion fruit, highlighting their significance in postharvest management and flavor quality enhancement strategies.https://www.mdpi.com/2223-7747/14/5/645passion fruitAP2/ERFpostharvest storage<i>PeSTP6</i>flavor
spellingShingle Lanjun Luo
Liping Zhang
Ronghao Gu
Shihao Ni
Jingyao Yu
Yachao Gao
Chuanying Fang
Genome-Wide Identification and Functional Analysis of <i>AP2/ERF</i> Gene Family in <i>Passiflora edulis</i> Sims
Plants
passion fruit
AP2/ERF
postharvest storage
<i>PeSTP6</i>
flavor
title Genome-Wide Identification and Functional Analysis of <i>AP2/ERF</i> Gene Family in <i>Passiflora edulis</i> Sims
title_full Genome-Wide Identification and Functional Analysis of <i>AP2/ERF</i> Gene Family in <i>Passiflora edulis</i> Sims
title_fullStr Genome-Wide Identification and Functional Analysis of <i>AP2/ERF</i> Gene Family in <i>Passiflora edulis</i> Sims
title_full_unstemmed Genome-Wide Identification and Functional Analysis of <i>AP2/ERF</i> Gene Family in <i>Passiflora edulis</i> Sims
title_short Genome-Wide Identification and Functional Analysis of <i>AP2/ERF</i> Gene Family in <i>Passiflora edulis</i> Sims
title_sort genome wide identification and functional analysis of i ap2 erf i gene family in i passiflora edulis i sims
topic passion fruit
AP2/ERF
postharvest storage
<i>PeSTP6</i>
flavor
url https://www.mdpi.com/2223-7747/14/5/645
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