New classes of mind bomb-interacting proteins identified from yeast two-hybrid screens.
Notch signaling pathway defines an evolutionarily conserved mechanism in cell-fate determination in a broad spectrum of developmental processes through local cell interactions. mind bomb (mib) encodes an E3 ubiquitin ligase that is involved in Notch activation through Delta ubiquitylation and intern...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093394&type=printable |
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| author | Li-Chuan Tseng Chengjin Zhang Chun-Mei Cheng Haoying Xu Chia-Hao Hsu Yun-Jin Jiang |
| author_facet | Li-Chuan Tseng Chengjin Zhang Chun-Mei Cheng Haoying Xu Chia-Hao Hsu Yun-Jin Jiang |
| author_sort | Li-Chuan Tseng |
| collection | DOAJ |
| description | Notch signaling pathway defines an evolutionarily conserved mechanism in cell-fate determination in a broad spectrum of developmental processes through local cell interactions. mind bomb (mib) encodes an E3 ubiquitin ligase that is involved in Notch activation through Delta ubiquitylation and internalization. To further dissect the function of Mib, two yeast two-hybrid screens for zebrafish Mib/Mib2-binding proteins with different strategies have been performed. 81 putative interesting proteins were discovered and classified into six groups: ubiquitin proteasome pathway, cytoskeleton, trafficking, replication/transcription/translation factors, cell signaling and others. Confirmed by coimmunoprecipitation (Co-IP), Mib interacted with four tested proteins: ubiquitin specific protease 1 (Usp1), ubiquitin specific protease 9 (Usp9), tumor-necrosis-factor-receptor-associated factor (TRAF)-binding domain (Trabid)/zinc finger, RAN-binding domain containing 1 (Zranb1) and hypoxia-inducible factor 1, alpha subunit inhibitor (Hif1an)/factor inhibiting HIF 1 (Fih-1). Usp1, Usp9, Trabid and Fih-1 also bound to zebrafish Mib2, a Mib homolog with similar structural domains and functions. Both Mib and Mib2 can ubiquitylate Trabid and Fih-1, indicating a potential regulating role of Mib and Mib2 on Trabid and Fih-1 and, furthermore, the possible involvement of Notch signaling in hypoxia-regulated differentiation, tumorigenesis and NF-κB pathway. Finally, functions of confirmed Mib/Mib2-interacting proteins are collated, summarized and hypothesized, which depicts a regulating network beyond Notch signaling. |
| format | Article |
| id | doaj-art-d0e48ab3889341f3bef8c22ea236f43a |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-d0e48ab3889341f3bef8c22ea236f43a2025-08-20T02:14:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9339410.1371/journal.pone.0093394New classes of mind bomb-interacting proteins identified from yeast two-hybrid screens.Li-Chuan TsengChengjin ZhangChun-Mei ChengHaoying XuChia-Hao HsuYun-Jin JiangNotch signaling pathway defines an evolutionarily conserved mechanism in cell-fate determination in a broad spectrum of developmental processes through local cell interactions. mind bomb (mib) encodes an E3 ubiquitin ligase that is involved in Notch activation through Delta ubiquitylation and internalization. To further dissect the function of Mib, two yeast two-hybrid screens for zebrafish Mib/Mib2-binding proteins with different strategies have been performed. 81 putative interesting proteins were discovered and classified into six groups: ubiquitin proteasome pathway, cytoskeleton, trafficking, replication/transcription/translation factors, cell signaling and others. Confirmed by coimmunoprecipitation (Co-IP), Mib interacted with four tested proteins: ubiquitin specific protease 1 (Usp1), ubiquitin specific protease 9 (Usp9), tumor-necrosis-factor-receptor-associated factor (TRAF)-binding domain (Trabid)/zinc finger, RAN-binding domain containing 1 (Zranb1) and hypoxia-inducible factor 1, alpha subunit inhibitor (Hif1an)/factor inhibiting HIF 1 (Fih-1). Usp1, Usp9, Trabid and Fih-1 also bound to zebrafish Mib2, a Mib homolog with similar structural domains and functions. Both Mib and Mib2 can ubiquitylate Trabid and Fih-1, indicating a potential regulating role of Mib and Mib2 on Trabid and Fih-1 and, furthermore, the possible involvement of Notch signaling in hypoxia-regulated differentiation, tumorigenesis and NF-κB pathway. Finally, functions of confirmed Mib/Mib2-interacting proteins are collated, summarized and hypothesized, which depicts a regulating network beyond Notch signaling.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093394&type=printable |
| spellingShingle | Li-Chuan Tseng Chengjin Zhang Chun-Mei Cheng Haoying Xu Chia-Hao Hsu Yun-Jin Jiang New classes of mind bomb-interacting proteins identified from yeast two-hybrid screens. PLoS ONE |
| title | New classes of mind bomb-interacting proteins identified from yeast two-hybrid screens. |
| title_full | New classes of mind bomb-interacting proteins identified from yeast two-hybrid screens. |
| title_fullStr | New classes of mind bomb-interacting proteins identified from yeast two-hybrid screens. |
| title_full_unstemmed | New classes of mind bomb-interacting proteins identified from yeast two-hybrid screens. |
| title_short | New classes of mind bomb-interacting proteins identified from yeast two-hybrid screens. |
| title_sort | new classes of mind bomb interacting proteins identified from yeast two hybrid screens |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093394&type=printable |
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