Modulatory Action by the Serotonergic System: Behavior and Neurophysiology in Drosophila melanogaster
Serotonin modulates various physiological processes and behaviors. This study investigates the role of 5-HT in locomotion and feeding behaviors as well as in modulation of sensory-motor circuits. The 5-HT biosynthesis was dysregulated by feeding Drosophila larvae 5-HT, a 5-HT precursor, or an inhibi...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/7291438 |
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| author | Zana R. Majeed Esraa Abdeljaber Robin Soveland Kristin Cornwell Aubrey Bankemper Felicitas Koch Robin L. Cooper |
| author_facet | Zana R. Majeed Esraa Abdeljaber Robin Soveland Kristin Cornwell Aubrey Bankemper Felicitas Koch Robin L. Cooper |
| author_sort | Zana R. Majeed |
| collection | DOAJ |
| description | Serotonin modulates various physiological processes and behaviors. This study investigates the role of 5-HT in locomotion and feeding behaviors as well as in modulation of sensory-motor circuits. The 5-HT biosynthesis was dysregulated by feeding Drosophila larvae 5-HT, a 5-HT precursor, or an inhibitor of tryptophan hydroxylase during early stages of development. The effects of feeding fluoxetine, a selective serotonin reuptake inhibitor, during early second instars were also examined. 5-HT receptor subtypes were manipulated using RNA interference mediated knockdown and 5-HT receptor insertional mutations. Moreover, synaptic transmission at 5-HT neurons was blocked or enhanced in both larvae and adult flies. The results demonstrate that disruption of components within the 5-HT system significantly impairs locomotion and feeding behaviors in larvae. Acute activation of 5-HT neurons disrupts normal locomotion activity in adult flies. To determine which 5-HT receptor subtype modulates the evoked sensory-motor activity, pharmacological agents were used. In addition, the activity of 5-HT neurons was enhanced by expressing and activating TrpA1 channels or channelrhodopsin-2 while recording the evoked excitatory postsynaptic potentials (EPSPs) in muscle fibers. 5-HT2 receptor activation mediates a modulatory role in a sensory-motor circuit, and the activation of 5-HT neurons can suppress the neural circuit activity, while fluoxetine can significantly decrease the sensory-motor activity. |
| format | Article |
| id | doaj-art-d0e3c4f6177e4c16b521d279b99d2d49 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-d0e3c4f6177e4c16b521d279b99d2d492025-02-03T05:53:49ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/72914387291438Modulatory Action by the Serotonergic System: Behavior and Neurophysiology in Drosophila melanogasterZana R. Majeed0Esraa Abdeljaber1Robin Soveland2Kristin Cornwell3Aubrey Bankemper4Felicitas Koch5Robin L. Cooper6Department of Biology, University of Kentucky, Lexington, KY 40506, USADepartment of Biology, University of Kentucky, Lexington, KY 40506, USADepartment of Biology, University of Kentucky, Lexington, KY 40506, USADepartment of Biology, University of Kentucky, Lexington, KY 40506, USADepartment of Biology, University of Kentucky, Lexington, KY 40506, USAVeterinärmedizinische Fakultät, Universität Leipzig, 04103 Leipzig, GermanyDepartment of Biology, University of Kentucky, Lexington, KY 40506, USASerotonin modulates various physiological processes and behaviors. This study investigates the role of 5-HT in locomotion and feeding behaviors as well as in modulation of sensory-motor circuits. The 5-HT biosynthesis was dysregulated by feeding Drosophila larvae 5-HT, a 5-HT precursor, or an inhibitor of tryptophan hydroxylase during early stages of development. The effects of feeding fluoxetine, a selective serotonin reuptake inhibitor, during early second instars were also examined. 5-HT receptor subtypes were manipulated using RNA interference mediated knockdown and 5-HT receptor insertional mutations. Moreover, synaptic transmission at 5-HT neurons was blocked or enhanced in both larvae and adult flies. The results demonstrate that disruption of components within the 5-HT system significantly impairs locomotion and feeding behaviors in larvae. Acute activation of 5-HT neurons disrupts normal locomotion activity in adult flies. To determine which 5-HT receptor subtype modulates the evoked sensory-motor activity, pharmacological agents were used. In addition, the activity of 5-HT neurons was enhanced by expressing and activating TrpA1 channels or channelrhodopsin-2 while recording the evoked excitatory postsynaptic potentials (EPSPs) in muscle fibers. 5-HT2 receptor activation mediates a modulatory role in a sensory-motor circuit, and the activation of 5-HT neurons can suppress the neural circuit activity, while fluoxetine can significantly decrease the sensory-motor activity.http://dx.doi.org/10.1155/2016/7291438 |
| spellingShingle | Zana R. Majeed Esraa Abdeljaber Robin Soveland Kristin Cornwell Aubrey Bankemper Felicitas Koch Robin L. Cooper Modulatory Action by the Serotonergic System: Behavior and Neurophysiology in Drosophila melanogaster Neural Plasticity |
| title | Modulatory Action by the Serotonergic System: Behavior and Neurophysiology in Drosophila melanogaster |
| title_full | Modulatory Action by the Serotonergic System: Behavior and Neurophysiology in Drosophila melanogaster |
| title_fullStr | Modulatory Action by the Serotonergic System: Behavior and Neurophysiology in Drosophila melanogaster |
| title_full_unstemmed | Modulatory Action by the Serotonergic System: Behavior and Neurophysiology in Drosophila melanogaster |
| title_short | Modulatory Action by the Serotonergic System: Behavior and Neurophysiology in Drosophila melanogaster |
| title_sort | modulatory action by the serotonergic system behavior and neurophysiology in drosophila melanogaster |
| url | http://dx.doi.org/10.1155/2016/7291438 |
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