Determination Micro Spectrohotometric of Pyridoxine Hydrochloride and Nicotinamide via Derivative Spectrophotometry First and Second

A simple, sensitive and accurate spectrophotometric method for determination of PYR.HCl and NTA in pure and pharmaceutical forms. The method based on group of known digital data used to separate the response signals for sample contains the two components without need to separating via first and sec...

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Main Authors: Mohammed Qasim Al-Sammaraee, Khlaf Faris Al-Sammaraee
Format: Article
Language:English
Published: Tikrit University 2023-02-01
Series:Tikrit Journal of Pure Science
Subjects:
Online Access:https://tjpsj.org/index.php/tjps/article/view/934
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author Mohammed Qasim Al-Sammaraee
Khlaf Faris Al-Sammaraee
author_facet Mohammed Qasim Al-Sammaraee
Khlaf Faris Al-Sammaraee
author_sort Mohammed Qasim Al-Sammaraee
collection DOAJ
description A simple, sensitive and accurate spectrophotometric method for determination of PYR.HCl and NTA in pure and pharmaceutical forms. The method based on group of known digital data used to separate the response signals for sample contains the two components without need to separating via first and second derivative of spectra. The method obtained obeying to the Beers law in the concentration range between )5-45 µg.ml-1( for two components in the presence of PYR. HCl )15µg.ml-1( and NTA (5µg.ml-1) with another components at wavelength (210 nm ) respectively . The Rec % range was between ) 98.5354 - 103.4335 %  (and RSD% (0.0864 - 0.1487 %), LOD) 1.0822 µg.ml-1, (  LOQ ( 3.6075 µg.ml-1  (PYR.HCl in presence of NTA, The Rec% range was between )96.1418 - 102.9273 %  (and RSD% (0.2165–0.4933 % ), LOD (0.7273µg.ml-1 ,(LOQ (2.4252 µg.ml-1(  NTA in presence of  PYR. HCl .
format Article
id doaj-art-d0e05338402a4068942fb8603d2a8971
institution Kabale University
issn 1813-1662
2415-1726
language English
publishDate 2023-02-01
publisher Tikrit University
record_format Article
series Tikrit Journal of Pure Science
spelling doaj-art-d0e05338402a4068942fb8603d2a89712025-08-20T03:28:59ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262023-02-01221210.25130/tjps.v22i12.934Determination Micro Spectrohotometric of Pyridoxine Hydrochloride and Nicotinamide via Derivative Spectrophotometry First and SecondMohammed Qasim Al-SammaraeeKhlaf Faris Al-Sammaraee A simple, sensitive and accurate spectrophotometric method for determination of PYR.HCl and NTA in pure and pharmaceutical forms. The method based on group of known digital data used to separate the response signals for sample contains the two components without need to separating via first and second derivative of spectra. The method obtained obeying to the Beers law in the concentration range between )5-45 µg.ml-1( for two components in the presence of PYR. HCl )15µg.ml-1( and NTA (5µg.ml-1) with another components at wavelength (210 nm ) respectively . The Rec % range was between ) 98.5354 - 103.4335 %  (and RSD% (0.0864 - 0.1487 %), LOD) 1.0822 µg.ml-1, (  LOQ ( 3.6075 µg.ml-1  (PYR.HCl in presence of NTA, The Rec% range was between )96.1418 - 102.9273 %  (and RSD% (0.2165–0.4933 % ), LOD (0.7273µg.ml-1 ,(LOQ (2.4252 µg.ml-1(  NTA in presence of  PYR. HCl . https://tjpsj.org/index.php/tjps/article/view/934derivative of spectraPyridoxine HydrochlorideNicotinamide
spellingShingle Mohammed Qasim Al-Sammaraee
Khlaf Faris Al-Sammaraee
Determination Micro Spectrohotometric of Pyridoxine Hydrochloride and Nicotinamide via Derivative Spectrophotometry First and Second
Tikrit Journal of Pure Science
derivative of spectra
Pyridoxine Hydrochloride
Nicotinamide
title Determination Micro Spectrohotometric of Pyridoxine Hydrochloride and Nicotinamide via Derivative Spectrophotometry First and Second
title_full Determination Micro Spectrohotometric of Pyridoxine Hydrochloride and Nicotinamide via Derivative Spectrophotometry First and Second
title_fullStr Determination Micro Spectrohotometric of Pyridoxine Hydrochloride and Nicotinamide via Derivative Spectrophotometry First and Second
title_full_unstemmed Determination Micro Spectrohotometric of Pyridoxine Hydrochloride and Nicotinamide via Derivative Spectrophotometry First and Second
title_short Determination Micro Spectrohotometric of Pyridoxine Hydrochloride and Nicotinamide via Derivative Spectrophotometry First and Second
title_sort determination micro spectrohotometric of pyridoxine hydrochloride and nicotinamide via derivative spectrophotometry first and second
topic derivative of spectra
Pyridoxine Hydrochloride
Nicotinamide
url https://tjpsj.org/index.php/tjps/article/view/934
work_keys_str_mv AT mohammedqasimalsammaraee determinationmicrospectrohotometricofpyridoxinehydrochlorideandnicotinamideviaderivativespectrophotometryfirstandsecond
AT khlaffarisalsammaraee determinationmicrospectrohotometricofpyridoxinehydrochlorideandnicotinamideviaderivativespectrophotometryfirstandsecond