Estimation of Levetiracetam in Tablet Dosage Form by RP-HPLC
A simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of levetiracetam in tablet dosage form. A Sun Fire C18, 250 x 4.6 mm, 5 μm partical size, with mobile phase consisting of acetonitrile and 0.03 M potassium dihydrogen phosphate (pH adjusted to 3.0 with ortho...
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| Format: | Article |
| Language: | English |
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Wiley
2008-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2008/965430 |
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| author | N. Appala Raju J. Venkateswara Rao K. Vanitha Prakash K. Mukkanti K. Srinivasu |
| author_facet | N. Appala Raju J. Venkateswara Rao K. Vanitha Prakash K. Mukkanti K. Srinivasu |
| author_sort | N. Appala Raju |
| collection | DOAJ |
| description | A simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of levetiracetam in tablet dosage form. A Sun Fire C18, 250 x 4.6 mm, 5 μm partical size, with mobile phase consisting of acetonitrile and 0.03 M potassium dihydrogen phosphate (pH adjusted to 3.0 with orthophosphoric acid) in the ratio of 15:85 v/v was used. The flow rate was 1 mL /min and the effluents were monitored at 210 nm. The retention time was 5.53 min. The detector response was linear in the concentration of 20-240 μg/mL. The respective linear regression equation being Y= 22119.684 x 6829.3428. The limit of detection and limit of quantification was 0.16 and 0.5 μg/mL respectively. The percentage assay of levetiracetam was 99.87%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of levetiracetam in bulk drug and in its pharmaceutical dosage form. |
| format | Article |
| id | doaj-art-d0dd653162a8468fbb48eaf23b65c74f |
| institution | DOAJ |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-d0dd653162a8468fbb48eaf23b65c74f2025-08-20T03:19:35ZengWileyE-Journal of Chemistry0973-49452090-98102008-01-015S21098110210.1155/2008/965430Estimation of Levetiracetam in Tablet Dosage Form by RP-HPLCN. Appala Raju0J. Venkateswara Rao1K. Vanitha Prakash2K. Mukkanti3K. Srinivasu4Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road No. 3, Banjara Hills, Hyderabad-500 034, IndiaDepartment of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road No. 3, Banjara Hills, Hyderabad-500 034, IndiaDepartment of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road No. 3, Banjara Hills, Hyderabad-500 034, IndiaCentre For Environment, IST Building, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad -500072, IndiaDr.Reddy's Laboratories, Analytical R&D, Hyderabad, IndiaA simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of levetiracetam in tablet dosage form. A Sun Fire C18, 250 x 4.6 mm, 5 μm partical size, with mobile phase consisting of acetonitrile and 0.03 M potassium dihydrogen phosphate (pH adjusted to 3.0 with orthophosphoric acid) in the ratio of 15:85 v/v was used. The flow rate was 1 mL /min and the effluents were monitored at 210 nm. The retention time was 5.53 min. The detector response was linear in the concentration of 20-240 μg/mL. The respective linear regression equation being Y= 22119.684 x 6829.3428. The limit of detection and limit of quantification was 0.16 and 0.5 μg/mL respectively. The percentage assay of levetiracetam was 99.87%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of levetiracetam in bulk drug and in its pharmaceutical dosage form.http://dx.doi.org/10.1155/2008/965430 |
| spellingShingle | N. Appala Raju J. Venkateswara Rao K. Vanitha Prakash K. Mukkanti K. Srinivasu Estimation of Levetiracetam in Tablet Dosage Form by RP-HPLC E-Journal of Chemistry |
| title | Estimation of Levetiracetam in Tablet Dosage Form by RP-HPLC |
| title_full | Estimation of Levetiracetam in Tablet Dosage Form by RP-HPLC |
| title_fullStr | Estimation of Levetiracetam in Tablet Dosage Form by RP-HPLC |
| title_full_unstemmed | Estimation of Levetiracetam in Tablet Dosage Form by RP-HPLC |
| title_short | Estimation of Levetiracetam in Tablet Dosage Form by RP-HPLC |
| title_sort | estimation of levetiracetam in tablet dosage form by rp hplc |
| url | http://dx.doi.org/10.1155/2008/965430 |
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