Pooled genetic screens with image‐based profiling
Abstract Spatial structure in biology, spanning molecular, organellular, cellular, tissue, and organismal scales, is encoded through a combination of genetic and epigenetic factors in individual cells. Microscopy remains the most direct approach to exploring the intricate spatial complexity defining...
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| Format: | Article |
| Language: | English |
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Springer Nature
2022-11-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.15252/msb.202110768 |
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| author | Russell T Walton Avtar Singh Paul C Blainey |
| author_facet | Russell T Walton Avtar Singh Paul C Blainey |
| author_sort | Russell T Walton |
| collection | DOAJ |
| description | Abstract Spatial structure in biology, spanning molecular, organellular, cellular, tissue, and organismal scales, is encoded through a combination of genetic and epigenetic factors in individual cells. Microscopy remains the most direct approach to exploring the intricate spatial complexity defining biological systems and the structured dynamic responses of these systems to perturbations. Genetic screens with deep single‐cell profiling via image features or gene expression programs have the capacity to show how biological systems work in detail by cataloging many cellular phenotypes with one experimental assay. Microscopy‐based cellular profiling provides information complementary to next‐generation sequencing (NGS) profiling and has only recently become compatible with large‐scale genetic screens. Optical screening now offers the scale needed for systematic characterization and is poised for further scale‐up. We discuss how these methodologies, together with emerging technologies for genetic perturbation and microscopy‐based multiplexed molecular phenotyping, are powering new approaches to reveal genotype–phenotype relationships. |
| format | Article |
| id | doaj-art-d0dbc8fc7466427390ba183d21c1a17d |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-d0dbc8fc7466427390ba183d21c1a17d2025-08-24T12:01:01ZengSpringer NatureMolecular Systems Biology1744-42922022-11-01181112310.15252/msb.202110768Pooled genetic screens with image‐based profilingRussell T Walton0Avtar Singh1Paul C Blainey2Broad Institute of MIT and HarvardBroad Institute of MIT and HarvardBroad Institute of MIT and HarvardAbstract Spatial structure in biology, spanning molecular, organellular, cellular, tissue, and organismal scales, is encoded through a combination of genetic and epigenetic factors in individual cells. Microscopy remains the most direct approach to exploring the intricate spatial complexity defining biological systems and the structured dynamic responses of these systems to perturbations. Genetic screens with deep single‐cell profiling via image features or gene expression programs have the capacity to show how biological systems work in detail by cataloging many cellular phenotypes with one experimental assay. Microscopy‐based cellular profiling provides information complementary to next‐generation sequencing (NGS) profiling and has only recently become compatible with large‐scale genetic screens. Optical screening now offers the scale needed for systematic characterization and is poised for further scale‐up. We discuss how these methodologies, together with emerging technologies for genetic perturbation and microscopy‐based multiplexed molecular phenotyping, are powering new approaches to reveal genotype–phenotype relationships.https://doi.org/10.15252/msb.202110768morphological profilingmultiplexed imagingoptical microscopy profilingoptical screeningpooled genetic screening |
| spellingShingle | Russell T Walton Avtar Singh Paul C Blainey Pooled genetic screens with image‐based profiling Molecular Systems Biology morphological profiling multiplexed imaging optical microscopy profiling optical screening pooled genetic screening |
| title | Pooled genetic screens with image‐based profiling |
| title_full | Pooled genetic screens with image‐based profiling |
| title_fullStr | Pooled genetic screens with image‐based profiling |
| title_full_unstemmed | Pooled genetic screens with image‐based profiling |
| title_short | Pooled genetic screens with image‐based profiling |
| title_sort | pooled genetic screens with image based profiling |
| topic | morphological profiling multiplexed imaging optical microscopy profiling optical screening pooled genetic screening |
| url | https://doi.org/10.15252/msb.202110768 |
| work_keys_str_mv | AT russelltwalton pooledgeneticscreenswithimagebasedprofiling AT avtarsingh pooledgeneticscreenswithimagebasedprofiling AT paulcblainey pooledgeneticscreenswithimagebasedprofiling |