QCM Analysis for Two-Step Adsorption of Albumin and Fibronectin on Zirconia Surface

The adsorption of proteins on the dental implant surface is the first step in the key role of osseointegration. Many types of proteins exist in the living body and compete for adsorption on the material surface. As an implant material, partially stabilized zirconia (ZrO2) is currently an attractive...

Full description

Saved in:
Bibliographic Details
Main Authors: Masatsugu Hirota, Tohru Hayakawa
Format: Article
Language:English
Published: Wiley 2021-01-01
Series:Advances in Materials Science and Engineering
Online Access:http://dx.doi.org/10.1155/2021/2492387
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832561531817033728
author Masatsugu Hirota
Tohru Hayakawa
author_facet Masatsugu Hirota
Tohru Hayakawa
author_sort Masatsugu Hirota
collection DOAJ
description The adsorption of proteins on the dental implant surface is the first step in the key role of osseointegration. Many types of proteins exist in the living body and compete for adsorption on the material surface. As an implant material, partially stabilized zirconia (ZrO2) is currently an attractive alternative to titanium to overcome the shortcomings of titanium implants. In this study, we investigated the two-step adsorption of fibronectin (Fn) and bovine serum albumin (Alb) on the ZrO2 surface using a 27-MHz quartz crystal microbalance (QCM) method. A ZrO2 sensor was employed for the QCM measurements. Two-step adsorptions were performed as follows. (1) Fn-Alb series: first, the Fn solution was injected into the phosphate-buffered saline (PBS) solution, followed by the Alb solution. (2) Alb-Fn series: first, the Alb solution was injected, followed by the Fn solution. The decrease in frequency was monitored for 60 minutes after each protein injection. The adsorbed amounts of Fn or Alb were calculated by observing the decrease in frequency, and the apparent reaction rate, kobs, was obtained through the curve fitting of frequency shift against the adsorption time. No significant difference was observed in the adsorbed amounts of Fn and Alb between the Fn-Alb and Alb-Fn series (P>0.05). The kobs, rate of protein adsorption, in the second step was significantly slower than that in the first step for both Fn and Alb adsorption (P<0.05). There was no clear correlation between the amount of protein adsorbed on the ZrO2 sensor and the surface topography. It was concluded that the amount of protein adsorbed on the ZrO2 surface was not influenced by the two-step adsorption series, but the adsorption rate of proteins in the second step was affected by the first-step protein adsorption.
format Article
id doaj-art-d0d759bf8634415493aa963e8ca519d7
institution Kabale University
issn 1687-8434
1687-8442
language English
publishDate 2021-01-01
publisher Wiley
record_format Article
series Advances in Materials Science and Engineering
spelling doaj-art-d0d759bf8634415493aa963e8ca519d72025-02-03T01:24:50ZengWileyAdvances in Materials Science and Engineering1687-84341687-84422021-01-01202110.1155/2021/24923872492387QCM Analysis for Two-Step Adsorption of Albumin and Fibronectin on Zirconia SurfaceMasatsugu Hirota0Tohru Hayakawa1Department of Education for Dental Medicine, Tsurumi University School of Dental Medicine, Yokohama, Kanagawa, JapanDepartment of Dental Engineering, Tsurumi University School of Dental Medicine, Yokohama, Kanagawa, JapanThe adsorption of proteins on the dental implant surface is the first step in the key role of osseointegration. Many types of proteins exist in the living body and compete for adsorption on the material surface. As an implant material, partially stabilized zirconia (ZrO2) is currently an attractive alternative to titanium to overcome the shortcomings of titanium implants. In this study, we investigated the two-step adsorption of fibronectin (Fn) and bovine serum albumin (Alb) on the ZrO2 surface using a 27-MHz quartz crystal microbalance (QCM) method. A ZrO2 sensor was employed for the QCM measurements. Two-step adsorptions were performed as follows. (1) Fn-Alb series: first, the Fn solution was injected into the phosphate-buffered saline (PBS) solution, followed by the Alb solution. (2) Alb-Fn series: first, the Alb solution was injected, followed by the Fn solution. The decrease in frequency was monitored for 60 minutes after each protein injection. The adsorbed amounts of Fn or Alb were calculated by observing the decrease in frequency, and the apparent reaction rate, kobs, was obtained through the curve fitting of frequency shift against the adsorption time. No significant difference was observed in the adsorbed amounts of Fn and Alb between the Fn-Alb and Alb-Fn series (P>0.05). The kobs, rate of protein adsorption, in the second step was significantly slower than that in the first step for both Fn and Alb adsorption (P<0.05). There was no clear correlation between the amount of protein adsorbed on the ZrO2 sensor and the surface topography. It was concluded that the amount of protein adsorbed on the ZrO2 surface was not influenced by the two-step adsorption series, but the adsorption rate of proteins in the second step was affected by the first-step protein adsorption.http://dx.doi.org/10.1155/2021/2492387
spellingShingle Masatsugu Hirota
Tohru Hayakawa
QCM Analysis for Two-Step Adsorption of Albumin and Fibronectin on Zirconia Surface
Advances in Materials Science and Engineering
title QCM Analysis for Two-Step Adsorption of Albumin and Fibronectin on Zirconia Surface
title_full QCM Analysis for Two-Step Adsorption of Albumin and Fibronectin on Zirconia Surface
title_fullStr QCM Analysis for Two-Step Adsorption of Albumin and Fibronectin on Zirconia Surface
title_full_unstemmed QCM Analysis for Two-Step Adsorption of Albumin and Fibronectin on Zirconia Surface
title_short QCM Analysis for Two-Step Adsorption of Albumin and Fibronectin on Zirconia Surface
title_sort qcm analysis for two step adsorption of albumin and fibronectin on zirconia surface
url http://dx.doi.org/10.1155/2021/2492387
work_keys_str_mv AT masatsuguhirota qcmanalysisfortwostepadsorptionofalbuminandfibronectinonzirconiasurface
AT tohruhayakawa qcmanalysisfortwostepadsorptionofalbuminandfibronectinonzirconiasurface