Evaluation of serum hsa_tsr014055 as a potential biomarker for diagnosis and prognosis of hepatocellular carcinoma
Background Mounting evidence has shown that tRNA-derived small RNAs (tsRNAs) play critical roles in the progression of tumors, including hepatocellular carcinoma (HCC). Nevertheless, the clinical values of serum tsRNAs for HCC are still largely unknown.Methods Serum samples were procured from 153 HC...
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Taylor & Francis Group
2025-12-01
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| Series: | Annals of Medicine |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/07853890.2025.2528978 |
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| author | Jing Xu Bairong Chen Jing Qi Jinran Wu Wei Feng Kangfeng Jin Hongyi Bao Lin Chen Feng Wang |
| author_facet | Jing Xu Bairong Chen Jing Qi Jinran Wu Wei Feng Kangfeng Jin Hongyi Bao Lin Chen Feng Wang |
| author_sort | Jing Xu |
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| description | Background Mounting evidence has shown that tRNA-derived small RNAs (tsRNAs) play critical roles in the progression of tumors, including hepatocellular carcinoma (HCC). Nevertheless, the clinical values of serum tsRNAs for HCC are still largely unknown.Methods Serum samples were procured from 153 HCC patients, 93 hepatitis cases, and 89 healthy donors from the Affiliated Hospital of Nantong University. A series of analyses, including qRT-PCR, receiver operating characteristic (ROC) curve, and Kaplan-Meier analysis, were performed to investigate the potential performance of serum tsRNA-Val-5-0035, also known as hsa_tsr014055, for HCC clinical practice.Results Methodological evaluation showed that qRT-PCR detection of serum hsa_tsr014055 had better stability, repeatability, and primer specificity. Moreover, serum hsa_tsr014055 was significantly higher in HCC patients than in hepatitis cases and healthy donors. Dynamic monitoring disclosed that it was dropped in HCC patients who had undergone surgery. Besides, it was positively correlated with TNM stage, differentiation grade, and microvascular invasion, as well as the prognosis of HCC patients. Additionally, serum hsa_tsr014055 had potential diagnostic value for HCC. Importantly, when it was combined with alpha-fetoprotein (AFP) and des-γ-carboxyprothrombin (DCP), the diagnostic efficacy was obviously improved. Also, bioinformatic prediction demonstrated that hsa_tsr014055 had 717 downstream targets, which were enriched in Hedgehog and cAMP signaling pathways.Conclusion Serum hsa_tsr014055 may be a promising biomarker in the diagnosis, evaluation of therapeutic effects, and assessment of HCC prognosis. Especially when serum hsa_tsr014055 was combined with AFP and DCP, the diagnostic efficacy for HCC would be enhanced. |
| format | Article |
| id | doaj-art-d0d1a152bc364b128e6c8f37cca9ca1f |
| institution | Kabale University |
| issn | 0785-3890 1365-2060 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Annals of Medicine |
| spelling | doaj-art-d0d1a152bc364b128e6c8f37cca9ca1f2025-08-20T03:31:48ZengTaylor & Francis GroupAnnals of Medicine0785-38901365-20602025-12-0157110.1080/07853890.2025.2528978Evaluation of serum hsa_tsr014055 as a potential biomarker for diagnosis and prognosis of hepatocellular carcinomaJing Xu0Bairong Chen1Jing Qi2Jinran Wu3Wei Feng4Kangfeng Jin5Hongyi Bao6Lin Chen7Feng Wang8Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, ChinaResearch Center of Clinical Medicine, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, ChinaResearch Center of Clinical Medicine, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, ChinaResearch Center of Clinical Medicine, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, ChinaResearch Center of Clinical Medicine, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, ChinaResearch Center of Clinical Medicine, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, ChinaResearch Center of Clinical Medicine, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, ChinaNantong Institute of Liver Diseases, Nantong Third People’s Hospital Affiliated Nantong Hospital 3 of Nantong University, Nantong, ChinaResearch Center of Clinical Medicine, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, ChinaBackground Mounting evidence has shown that tRNA-derived small RNAs (tsRNAs) play critical roles in the progression of tumors, including hepatocellular carcinoma (HCC). Nevertheless, the clinical values of serum tsRNAs for HCC are still largely unknown.Methods Serum samples were procured from 153 HCC patients, 93 hepatitis cases, and 89 healthy donors from the Affiliated Hospital of Nantong University. A series of analyses, including qRT-PCR, receiver operating characteristic (ROC) curve, and Kaplan-Meier analysis, were performed to investigate the potential performance of serum tsRNA-Val-5-0035, also known as hsa_tsr014055, for HCC clinical practice.Results Methodological evaluation showed that qRT-PCR detection of serum hsa_tsr014055 had better stability, repeatability, and primer specificity. Moreover, serum hsa_tsr014055 was significantly higher in HCC patients than in hepatitis cases and healthy donors. Dynamic monitoring disclosed that it was dropped in HCC patients who had undergone surgery. Besides, it was positively correlated with TNM stage, differentiation grade, and microvascular invasion, as well as the prognosis of HCC patients. Additionally, serum hsa_tsr014055 had potential diagnostic value for HCC. Importantly, when it was combined with alpha-fetoprotein (AFP) and des-γ-carboxyprothrombin (DCP), the diagnostic efficacy was obviously improved. Also, bioinformatic prediction demonstrated that hsa_tsr014055 had 717 downstream targets, which were enriched in Hedgehog and cAMP signaling pathways.Conclusion Serum hsa_tsr014055 may be a promising biomarker in the diagnosis, evaluation of therapeutic effects, and assessment of HCC prognosis. Especially when serum hsa_tsr014055 was combined with AFP and DCP, the diagnostic efficacy for HCC would be enhanced.https://www.tandfonline.com/doi/10.1080/07853890.2025.2528978Hepatocellular carcinomatRNA-derived small RNAshsa_tsr014055biomarkerdiagnosis |
| spellingShingle | Jing Xu Bairong Chen Jing Qi Jinran Wu Wei Feng Kangfeng Jin Hongyi Bao Lin Chen Feng Wang Evaluation of serum hsa_tsr014055 as a potential biomarker for diagnosis and prognosis of hepatocellular carcinoma Annals of Medicine Hepatocellular carcinoma tRNA-derived small RNAs hsa_tsr014055 biomarker diagnosis |
| title | Evaluation of serum hsa_tsr014055 as a potential biomarker for diagnosis and prognosis of hepatocellular carcinoma |
| title_full | Evaluation of serum hsa_tsr014055 as a potential biomarker for diagnosis and prognosis of hepatocellular carcinoma |
| title_fullStr | Evaluation of serum hsa_tsr014055 as a potential biomarker for diagnosis and prognosis of hepatocellular carcinoma |
| title_full_unstemmed | Evaluation of serum hsa_tsr014055 as a potential biomarker for diagnosis and prognosis of hepatocellular carcinoma |
| title_short | Evaluation of serum hsa_tsr014055 as a potential biomarker for diagnosis and prognosis of hepatocellular carcinoma |
| title_sort | evaluation of serum hsa tsr014055 as a potential biomarker for diagnosis and prognosis of hepatocellular carcinoma |
| topic | Hepatocellular carcinoma tRNA-derived small RNAs hsa_tsr014055 biomarker diagnosis |
| url | https://www.tandfonline.com/doi/10.1080/07853890.2025.2528978 |
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