Immunohistochemical and Molecular Characterization of the Human Periosteum
Purpose. The aim of the present study was to characterize the cell of the human periosteum using immunohistological and molecular methods. Methods. Phenotypic properties and the distribution of the cells within the different layers were investigated with immunohistochemical staining techniques and R...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/341078 |
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| author | Sönke Percy Frey Hendrik Jansen Stefanie Doht Luis Filgueira Rene Zellweger |
| author_facet | Sönke Percy Frey Hendrik Jansen Stefanie Doht Luis Filgueira Rene Zellweger |
| author_sort | Sönke Percy Frey |
| collection | DOAJ |
| description | Purpose. The aim of the present study was to characterize the cell of the human periosteum using immunohistological and molecular methods. Methods. Phenotypic properties and the distribution of the cells within the different layers were investigated with immunohistochemical staining techniques and RT-PCR, focussing on markers for stromal stem cells, osteoblasts, osteoclasts and immune cells. Results. Immunohistochemical results revealed that all stained cells were located in the cambium layer and that most cells were positive for vimentin. The majority of cells consisted of stromal stem cells and osteoblastic precursor cells. The density increased towards the deeper layers of the cambium. In addition, cells positive for markers of the osteoblast, chondrocyte, and osteoclast lineages were found. Interestingly, there were MHC class II-expressing immune cells suggesting the presence of dendritic cells. Using lineage-specific primer pairs RT-PCR confirmed the immunofluorescence microscopy results, supporting that human periosteum serves as a reservoir of stromal stem cells, as well as cells of the osteoblastic, and the chondroblastic lineage, osteoclasts, and dendritic cells. Conclusion. Our work elucidates the role of periosteum as a source of cells with a high regenerative capacity. Undifferentiated stromal stem cells as well as osteoblastic precursor cells are dominating in the cambium layer. A new outlook is given towards an immune response coming from the periosteum as MHC II positive immune cells were detected. |
| format | Article |
| id | doaj-art-d0c4d45421344ea6af07fa7b0a430ce0 |
| institution | DOAJ |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-d0c4d45421344ea6af07fa7b0a430ce02025-08-20T03:19:35ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/341078341078Immunohistochemical and Molecular Characterization of the Human PeriosteumSönke Percy Frey0Hendrik Jansen1Stefanie Doht2Luis Filgueira3Rene Zellweger4Department of Trauma, Hand, Plastic and Reconstructive Surgery, University of Würzburg, Oberdürrbacher Street 6, 97080 Würzburg, GermanyDepartment of Trauma, Hand, Plastic and Reconstructive Surgery, University of Würzburg, Oberdürrbacher Street 6, 97080 Würzburg, GermanyDepartment of Trauma, Hand, Plastic and Reconstructive Surgery, University of Würzburg, Oberdürrbacher Street 6, 97080 Würzburg, GermanyDepartment of Anatomy and Human Biology, University of Western Australia, Perth, WA 6009, AustraliaDepartment of Orthopedic and Trauma Surgery, University of Western Australia, Perth, WA 6000, AustraliaPurpose. The aim of the present study was to characterize the cell of the human periosteum using immunohistological and molecular methods. Methods. Phenotypic properties and the distribution of the cells within the different layers were investigated with immunohistochemical staining techniques and RT-PCR, focussing on markers for stromal stem cells, osteoblasts, osteoclasts and immune cells. Results. Immunohistochemical results revealed that all stained cells were located in the cambium layer and that most cells were positive for vimentin. The majority of cells consisted of stromal stem cells and osteoblastic precursor cells. The density increased towards the deeper layers of the cambium. In addition, cells positive for markers of the osteoblast, chondrocyte, and osteoclast lineages were found. Interestingly, there were MHC class II-expressing immune cells suggesting the presence of dendritic cells. Using lineage-specific primer pairs RT-PCR confirmed the immunofluorescence microscopy results, supporting that human periosteum serves as a reservoir of stromal stem cells, as well as cells of the osteoblastic, and the chondroblastic lineage, osteoclasts, and dendritic cells. Conclusion. Our work elucidates the role of periosteum as a source of cells with a high regenerative capacity. Undifferentiated stromal stem cells as well as osteoblastic precursor cells are dominating in the cambium layer. A new outlook is given towards an immune response coming from the periosteum as MHC II positive immune cells were detected.http://dx.doi.org/10.1155/2013/341078 |
| spellingShingle | Sönke Percy Frey Hendrik Jansen Stefanie Doht Luis Filgueira Rene Zellweger Immunohistochemical and Molecular Characterization of the Human Periosteum The Scientific World Journal |
| title | Immunohistochemical and Molecular Characterization of the Human Periosteum |
| title_full | Immunohistochemical and Molecular Characterization of the Human Periosteum |
| title_fullStr | Immunohistochemical and Molecular Characterization of the Human Periosteum |
| title_full_unstemmed | Immunohistochemical and Molecular Characterization of the Human Periosteum |
| title_short | Immunohistochemical and Molecular Characterization of the Human Periosteum |
| title_sort | immunohistochemical and molecular characterization of the human periosteum |
| url | http://dx.doi.org/10.1155/2013/341078 |
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