Assessing the role of field isolated Pseudomonas and Bacillus as growth‐promoting rizobacteria on avocado (Persea americana) seedlings
Abstract Introduction This research aims to assess the efficacy of two genera of rhizobacteria from avocado field isolated: Pseudomonas and Bacillus, as plant growth‐promoting microorganisms in Hass avocado trees grafted onto Zutano rootstock. Materials and Methods The siderophore‐producing and phos...
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Wiley
2024-09-01
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| Series: | Journal of Sustainable Agriculture and Environment |
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| Online Access: | https://doi.org/10.1002/sae2.12114 |
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| author | Richard A. Solórzano‐Acosta Kenyi R. Quispe |
| author_facet | Richard A. Solórzano‐Acosta Kenyi R. Quispe |
| author_sort | Richard A. Solórzano‐Acosta |
| collection | DOAJ |
| description | Abstract Introduction This research aims to assess the efficacy of two genera of rhizobacteria from avocado field isolated: Pseudomonas and Bacillus, as plant growth‐promoting microorganisms in Hass avocado trees grafted onto Zutano rootstock. Materials and Methods The siderophore‐producing and phosphate‐solubilizing capacity of each isolated strain was determined and plant growth‐promoting activity, nutrient accumulation, and nutrient use efficiency in Zutano variety avocado seedlings were evaluated. Molecular identification was carried out by amplification of the 16S rDNA gene of the isolated strains. Results Pseudomonas putida, Lysinibacillus macroides, Lysinibacillus xylanilyticus, Lysinibacillus fusiformis, Bacillus subtilis and Pseudomonas plecoglossicida, were identified as the PGPR of the Bacillus and Pseudomonas genera, predominant in the avocado rhizosphere. There was found 11 phosphate solubilizing strains and 2 siderophore‐producing strains. The phosphate‐solubilizing strains, B. subtilis and P. plecoglossicida, stimulated the growth of Zutano seedlings, increasing their root dry weight (g), stem dry weight (g), leaf dry weight (g) and leaf area (cm2). Significant differences were found in nutrient uptake efficiency between inoculated plants and noninoculated plants. The increase in root biomass responded to greater phosphorus and potassium absorption in plants inoculated with P. plecoglossicida, due to this strain's high phosphate solubilization efficiency (266%). Conclusions The highest plant growth promotion strains were Bac F (B. subtilis), Bac M (P. plecoglossicida) and P1 (P. putida), which achieved the highest increase in root and leaf dry weight, as well as the highest nutrient extractions and nutrient uptake efficiency. |
| format | Article |
| id | doaj-art-d0c1d44d32a04c398aa020f63f6d2f50 |
| institution | DOAJ |
| issn | 2767-035X |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Sustainable Agriculture and Environment |
| spelling | doaj-art-d0c1d44d32a04c398aa020f63f6d2f502025-08-20T03:00:51ZengWileyJournal of Sustainable Agriculture and Environment2767-035X2024-09-0133n/an/a10.1002/sae2.12114Assessing the role of field isolated Pseudomonas and Bacillus as growth‐promoting rizobacteria on avocado (Persea americana) seedlingsRichard A. Solórzano‐Acosta0Kenyi R. Quispe1Dirección de Supervisión y Monitoreo en las Estaciones Experimentales Agrarias Instituto Nacional de Innovación Agraria (INIA) Lima PerúDirección de Supervisión y Monitoreo en las Estaciones Experimentales Agrarias Instituto Nacional de Innovación Agraria (INIA) Lima PerúAbstract Introduction This research aims to assess the efficacy of two genera of rhizobacteria from avocado field isolated: Pseudomonas and Bacillus, as plant growth‐promoting microorganisms in Hass avocado trees grafted onto Zutano rootstock. Materials and Methods The siderophore‐producing and phosphate‐solubilizing capacity of each isolated strain was determined and plant growth‐promoting activity, nutrient accumulation, and nutrient use efficiency in Zutano variety avocado seedlings were evaluated. Molecular identification was carried out by amplification of the 16S rDNA gene of the isolated strains. Results Pseudomonas putida, Lysinibacillus macroides, Lysinibacillus xylanilyticus, Lysinibacillus fusiformis, Bacillus subtilis and Pseudomonas plecoglossicida, were identified as the PGPR of the Bacillus and Pseudomonas genera, predominant in the avocado rhizosphere. There was found 11 phosphate solubilizing strains and 2 siderophore‐producing strains. The phosphate‐solubilizing strains, B. subtilis and P. plecoglossicida, stimulated the growth of Zutano seedlings, increasing their root dry weight (g), stem dry weight (g), leaf dry weight (g) and leaf area (cm2). Significant differences were found in nutrient uptake efficiency between inoculated plants and noninoculated plants. The increase in root biomass responded to greater phosphorus and potassium absorption in plants inoculated with P. plecoglossicida, due to this strain's high phosphate solubilization efficiency (266%). Conclusions The highest plant growth promotion strains were Bac F (B. subtilis), Bac M (P. plecoglossicida) and P1 (P. putida), which achieved the highest increase in root and leaf dry weight, as well as the highest nutrient extractions and nutrient uptake efficiency.https://doi.org/10.1002/sae2.1211416S rDNA geneavocadonutrient uptake efficiencyPGPRrhizosphere |
| spellingShingle | Richard A. Solórzano‐Acosta Kenyi R. Quispe Assessing the role of field isolated Pseudomonas and Bacillus as growth‐promoting rizobacteria on avocado (Persea americana) seedlings Journal of Sustainable Agriculture and Environment 16S rDNA gene avocado nutrient uptake efficiency PGPR rhizosphere |
| title | Assessing the role of field isolated Pseudomonas and Bacillus as growth‐promoting rizobacteria on avocado (Persea americana) seedlings |
| title_full | Assessing the role of field isolated Pseudomonas and Bacillus as growth‐promoting rizobacteria on avocado (Persea americana) seedlings |
| title_fullStr | Assessing the role of field isolated Pseudomonas and Bacillus as growth‐promoting rizobacteria on avocado (Persea americana) seedlings |
| title_full_unstemmed | Assessing the role of field isolated Pseudomonas and Bacillus as growth‐promoting rizobacteria on avocado (Persea americana) seedlings |
| title_short | Assessing the role of field isolated Pseudomonas and Bacillus as growth‐promoting rizobacteria on avocado (Persea americana) seedlings |
| title_sort | assessing the role of field isolated pseudomonas and bacillus as growth promoting rizobacteria on avocado persea americana seedlings |
| topic | 16S rDNA gene avocado nutrient uptake efficiency PGPR rhizosphere |
| url | https://doi.org/10.1002/sae2.12114 |
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