The single-cell atlas of short-chain fatty acid receptors in human and mice hearts

IntroductionThe gut microbiota metabolite, short-chain fatty acids (SCFAs), can protect against multiple cardiovascular diseases, while the molecular targets and underlying mechanisms need to be elucidated. One of the primary mechanisms of SCFA benefits was the direct activation of a group of G-prot...

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Main Authors: Xiaojun He, Qiang Wang, Qiang Long, Yiming Zhong, Zhaoxi Qi, Yecen Zhang, Lan Chang, Bei Qian, Shixing Huang, Xinming Wang, Xuemei Chen, Feifei Li, Xiaomei Yang, Wei Dong Gao, Zhizhao Song, Li Xu, Qiang Zhao
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Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Immunology
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Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2025.1538384/full
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author Xiaojun He
Xiaojun He
Qiang Wang
Qiang Long
Yiming Zhong
Zhaoxi Qi
Yecen Zhang
Lan Chang
Bei Qian
Shixing Huang
Xinming Wang
Xuemei Chen
Feifei Li
Xiaomei Yang
Xiaomei Yang
Xiaomei Yang
Wei Dong Gao
Zhizhao Song
Li Xu
Qiang Zhao
author_facet Xiaojun He
Xiaojun He
Qiang Wang
Qiang Long
Yiming Zhong
Zhaoxi Qi
Yecen Zhang
Lan Chang
Bei Qian
Shixing Huang
Xinming Wang
Xuemei Chen
Feifei Li
Xiaomei Yang
Xiaomei Yang
Xiaomei Yang
Wei Dong Gao
Zhizhao Song
Li Xu
Qiang Zhao
author_sort Xiaojun He
collection DOAJ
description IntroductionThe gut microbiota metabolite, short-chain fatty acids (SCFAs), can protect against multiple cardiovascular diseases, while the molecular targets and underlying mechanisms need to be elucidated. One of the primary mechanisms of SCFA benefits was the direct activation of a group of G-protein-coupled receptors (GPCRs), termed free fatty acid receptors (FFARs), the FFAR2 (GPR43), and FFAR3 (GPR41). At present, the distribution of FFAR2/3 in cardiac cells has not been entirely clarified.MethodsUsing 18 public single-cell RNA-seq and single-nuclear RNA-seq data of human and mouse hearts, we illustrate the entire atlas of FFAR2/3 distribution in different regions and cell types in normal and infarcted hearts.Results and discussionWe present the atlas of FFAR2/3 in the whole human body, normal and infarcted hearts at single-cell resolution. We also illustrated the entire atlas of FFAR2/3 in normal/ischemic hearts of newborn and adult mice by combining public and newly built sc/snRNA-seq datasets. These findings provide valuable information on the possible effect of SCFAs via FFAR2/3 in the heart and valuable references for future studies.
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spelling doaj-art-d0c080c585ac4bbdba24f667612defff2025-08-20T02:26:36ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-04-011610.3389/fimmu.2025.15383841538384The single-cell atlas of short-chain fatty acid receptors in human and mice heartsXiaojun He0Xiaojun He1Qiang Wang2Qiang Long3Yiming Zhong4Zhaoxi Qi5Yecen Zhang6Lan Chang7Bei Qian8Shixing Huang9Xinming Wang10Xuemei Chen11Feifei Li12Xiaomei Yang13Xiaomei Yang14Xiaomei Yang15Wei Dong Gao16Zhizhao Song17Li Xu18Qiang Zhao19Cardiovascular Medical Center, Department of Cardiovascular Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, ChinaDepartment of Cardiovascular Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaCardiovascular Medical Center, Department of Cardiovascular Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, ChinaDepartment of Cardiovascular Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Cardiovascular Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Cardiovascular Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Cardiovascular Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Cardiovascular Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Cardiovascular Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Cardiovascular Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Cardiovascular Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Anesthesiology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Cardiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Anesthesiology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaSchool of Medicine, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Cardiology, Johns Hopkins School of Medicine, Baltimore, MD, United StatesDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United StatesCardiovascular Medical Center, Department of Cardiovascular Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, ChinaClinical Trial Institution, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu, ChinaDepartment of Cardiovascular Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaIntroductionThe gut microbiota metabolite, short-chain fatty acids (SCFAs), can protect against multiple cardiovascular diseases, while the molecular targets and underlying mechanisms need to be elucidated. One of the primary mechanisms of SCFA benefits was the direct activation of a group of G-protein-coupled receptors (GPCRs), termed free fatty acid receptors (FFARs), the FFAR2 (GPR43), and FFAR3 (GPR41). At present, the distribution of FFAR2/3 in cardiac cells has not been entirely clarified.MethodsUsing 18 public single-cell RNA-seq and single-nuclear RNA-seq data of human and mouse hearts, we illustrate the entire atlas of FFAR2/3 distribution in different regions and cell types in normal and infarcted hearts.Results and discussionWe present the atlas of FFAR2/3 in the whole human body, normal and infarcted hearts at single-cell resolution. We also illustrated the entire atlas of FFAR2/3 in normal/ischemic hearts of newborn and adult mice by combining public and newly built sc/snRNA-seq datasets. These findings provide valuable information on the possible effect of SCFAs via FFAR2/3 in the heart and valuable references for future studies.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1538384/fullFFAR2FFAR3single-cell atlashearthumanmice
spellingShingle Xiaojun He
Xiaojun He
Qiang Wang
Qiang Long
Yiming Zhong
Zhaoxi Qi
Yecen Zhang
Lan Chang
Bei Qian
Shixing Huang
Xinming Wang
Xuemei Chen
Feifei Li
Xiaomei Yang
Xiaomei Yang
Xiaomei Yang
Wei Dong Gao
Zhizhao Song
Li Xu
Qiang Zhao
The single-cell atlas of short-chain fatty acid receptors in human and mice hearts
Frontiers in Immunology
FFAR2
FFAR3
single-cell atlas
heart
human
mice
title The single-cell atlas of short-chain fatty acid receptors in human and mice hearts
title_full The single-cell atlas of short-chain fatty acid receptors in human and mice hearts
title_fullStr The single-cell atlas of short-chain fatty acid receptors in human and mice hearts
title_full_unstemmed The single-cell atlas of short-chain fatty acid receptors in human and mice hearts
title_short The single-cell atlas of short-chain fatty acid receptors in human and mice hearts
title_sort single cell atlas of short chain fatty acid receptors in human and mice hearts
topic FFAR2
FFAR3
single-cell atlas
heart
human
mice
url https://www.frontiersin.org/articles/10.3389/fimmu.2025.1538384/full
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