Zinc methionine mitigates intestinal mucosal injury of broilers challenged with Salmonella by modulating mucus secretion, tight junction and microbial composition
The investigation aimed to reveal potential mechanisms of zinc methionine on the intestinal mucosal function of Salmonella-challenged broilers. Totally, 320 Ross 308 male chicks were divided into 4 treatments, with 8 repetition per group and 10 chicks each. A 2 × 2 factor in a randomized block desig...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-11-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125009472 |
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| author | Yuxin Shao Yanrui Suo Zheng Wang Huiling Sun Yifang Cui Fangfang Guo Dongmei Lin Tenghe Ma Fuzhou Xu |
| author_facet | Yuxin Shao Yanrui Suo Zheng Wang Huiling Sun Yifang Cui Fangfang Guo Dongmei Lin Tenghe Ma Fuzhou Xu |
| author_sort | Yuxin Shao |
| collection | DOAJ |
| description | The investigation aimed to reveal potential mechanisms of zinc methionine on the intestinal mucosal function of Salmonella-challenged broilers. Totally, 320 Ross 308 male chicks were divided into 4 treatments, with 8 repetition per group and 10 chicks each. A 2 × 2 factor in a randomized block design was employed to explore the impacts of zinc addition, Salmonella challenge, and their interactions. The experiment lasted for 21 days. Results indicated that Salmonella infection decreased (P < 0.05) average daily gain (ADG) and the ratio of villus height to crypt depth (V/C) in both jejunum and ileum. The infection also reduced (P < 0.05) MUC2 and KLF4 mRNA levels, while elevating (P < 0.05) feed to gain (F:G), caudal-related homeobox transcriptional regulator 2 (CDX2) mRNA level, and altering (P < 0.05) bacterial composition. In contrast, zinc addition increased (P < 0.05) ADG, average daily feed intake, jejunal villus height, V/C, and tight junction mRNA expressions, but decreased (P < 0.05) F:G and CDX2 mRNA level. Moreover, zinc enhanced (P < 0.05) jejunal goblet cell number and ileal mucus thickness, and restored (P < 0.05) bacterial abundance. Correlation analysis found that zinc-enriched bacterial genera, including g_Butyricicoccus, g_Oscillibacter and g_Monoglobus were significantly positively correlated with broilers’ growth performance and intestinal morphology and tight junction Claudin-1 mRNA. CUT&Tag-seq analysis further demonstrated KLF4 binds to the exon region of MUC2 gene, but CDX2 did not bind to MUC2 promoter region. These suggest zinc mitigates Salmonella induced intestinal mucosal damage by promoting mucus secretion and tight junction mRNA expression, and microbial community reshaping, but not by improving KLF4-mediated MUC2 gene expression. |
| format | Article |
| id | doaj-art-d0bf20635ee149e2947857c3780b809e |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-d0bf20635ee149e2947857c3780b809e2025-08-24T05:11:14ZengElsevierPoultry Science0032-57912025-11-011041110570510.1016/j.psj.2025.105705Zinc methionine mitigates intestinal mucosal injury of broilers challenged with Salmonella by modulating mucus secretion, tight junction and microbial compositionYuxin Shao0Yanrui Suo1Zheng Wang2Huiling Sun3Yifang Cui4Fangfang Guo5Dongmei Lin6Tenghe Ma7Fuzhou Xu8Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaHebei University of Engineering, Handan, Hebei Province 056038, PR ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaHebei University of Engineering, Handan, Hebei Province 056038, PR ChinaHebei University of Engineering, Handan, Hebei Province 056038, PR ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; Corresponding author.The investigation aimed to reveal potential mechanisms of zinc methionine on the intestinal mucosal function of Salmonella-challenged broilers. Totally, 320 Ross 308 male chicks were divided into 4 treatments, with 8 repetition per group and 10 chicks each. A 2 × 2 factor in a randomized block design was employed to explore the impacts of zinc addition, Salmonella challenge, and their interactions. The experiment lasted for 21 days. Results indicated that Salmonella infection decreased (P < 0.05) average daily gain (ADG) and the ratio of villus height to crypt depth (V/C) in both jejunum and ileum. The infection also reduced (P < 0.05) MUC2 and KLF4 mRNA levels, while elevating (P < 0.05) feed to gain (F:G), caudal-related homeobox transcriptional regulator 2 (CDX2) mRNA level, and altering (P < 0.05) bacterial composition. In contrast, zinc addition increased (P < 0.05) ADG, average daily feed intake, jejunal villus height, V/C, and tight junction mRNA expressions, but decreased (P < 0.05) F:G and CDX2 mRNA level. Moreover, zinc enhanced (P < 0.05) jejunal goblet cell number and ileal mucus thickness, and restored (P < 0.05) bacterial abundance. Correlation analysis found that zinc-enriched bacterial genera, including g_Butyricicoccus, g_Oscillibacter and g_Monoglobus were significantly positively correlated with broilers’ growth performance and intestinal morphology and tight junction Claudin-1 mRNA. CUT&Tag-seq analysis further demonstrated KLF4 binds to the exon region of MUC2 gene, but CDX2 did not bind to MUC2 promoter region. These suggest zinc mitigates Salmonella induced intestinal mucosal damage by promoting mucus secretion and tight junction mRNA expression, and microbial community reshaping, but not by improving KLF4-mediated MUC2 gene expression.http://www.sciencedirect.com/science/article/pii/S0032579125009472Zinc methionineSalmonellaMucusIntestinal microbiota |
| spellingShingle | Yuxin Shao Yanrui Suo Zheng Wang Huiling Sun Yifang Cui Fangfang Guo Dongmei Lin Tenghe Ma Fuzhou Xu Zinc methionine mitigates intestinal mucosal injury of broilers challenged with Salmonella by modulating mucus secretion, tight junction and microbial composition Poultry Science Zinc methionine Salmonella Mucus Intestinal microbiota |
| title | Zinc methionine mitigates intestinal mucosal injury of broilers challenged with Salmonella by modulating mucus secretion, tight junction and microbial composition |
| title_full | Zinc methionine mitigates intestinal mucosal injury of broilers challenged with Salmonella by modulating mucus secretion, tight junction and microbial composition |
| title_fullStr | Zinc methionine mitigates intestinal mucosal injury of broilers challenged with Salmonella by modulating mucus secretion, tight junction and microbial composition |
| title_full_unstemmed | Zinc methionine mitigates intestinal mucosal injury of broilers challenged with Salmonella by modulating mucus secretion, tight junction and microbial composition |
| title_short | Zinc methionine mitigates intestinal mucosal injury of broilers challenged with Salmonella by modulating mucus secretion, tight junction and microbial composition |
| title_sort | zinc methionine mitigates intestinal mucosal injury of broilers challenged with salmonella by modulating mucus secretion tight junction and microbial composition |
| topic | Zinc methionine Salmonella Mucus Intestinal microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125009472 |
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