Phytochemical Profiling, GC-MS Analysis and Antibacterial Activity of Sida acuta leave Extracts Against Helicobacter pylori
Study’s Excerpt: • The antibacterial potential of Sida acuta against Helicobacter pylori is assessed. • Phytochemical profiling, GC-MS analysis, and antibacterial assays were integrated to determine the bioactive compounds in the plant. • The findings reveals that Sida acuta has antibacterial p...
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| Format: | Article |
| Language: | English |
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Umaru Musa Yar'adua University, Katsina, Nigeria
2024-12-01
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| Series: | UMYU Journal of Microbiology Research |
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| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/626 |
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| author | Kamoldeen Ajijolakewu Ayobami Elias Ajadi S A Ayoola Sheriffdeen Issa Bale Taiwo Ayodeji Sorunke Saheed Abayomi Onifade Tomisin Oyawoye |
| author_facet | Kamoldeen Ajijolakewu Ayobami Elias Ajadi S A Ayoola Sheriffdeen Issa Bale Taiwo Ayodeji Sorunke Saheed Abayomi Onifade Tomisin Oyawoye |
| author_sort | Kamoldeen Ajijolakewu |
| collection | DOAJ |
| description |
Study’s Excerpt:
• The antibacterial potential of Sida acuta against Helicobacter pylori is assessed.
• Phytochemical profiling, GC-MS analysis, and antibacterial assays were integrated to determine the bioactive compounds in the plant.
• The findings reveals that Sida acuta has antibacterial properties against ulcer-related H. pylori.
Full Abstract:
Helicobacter pylori is a major cause of ulcers, and increasing resistance to standard antibiotics underscores the need for alternative treatments. Sida acuta, a plant with medicinal properties, was examined in this study for its potential antibacterial effects. This research assessed the phytochemical profile, performed Gas Chromatography-Mass Spectrometry (GC-MS) analysis, and evaluated the antibacterial efficacy of Sida acuta extracts against H. pylori. Leaves of S. acuta were extracted using hexane, ethanol, and water, and their phytochemical constituents were analyzed through established qualitative and quantitative methods. The antibacterial activity of the leaf extracts was tested against H. pylori via agar well diffusion and broth dilution using various extract concentrations. GC-MS analysis was conducted to identify the bioactive compounds in each extract. Phytochemical screening revealed the presence of alkaloids, saponins, glycosides, tannins, flavonoids, triterpenoids, steroids, and phenols in the extracts. Aqueous extracts exhibited the largest inhibition zones (20.23±0.16 mm) at 500 mg/mL, followed by ethanol (18.25±0.25 mm) and hexane (16.35±0.25 mm) extracts. Minimum inhibitory concentrations (MIC) were determined as 62.5 mg/mL for ethanol, 125 mg/mL for water, and 250 mg/mL for hexane extracts. Bactericidal effects were observed with the ethanol and aqueous extracts at 250 mg/mL and 500 mg/mL, respectively, while hexane showed no bactericidal activity at tested concentrations. GC-MS identified 27 compounds in the ethanol extract, 20 in the aqueous extract, and 25 in the hexane extract. These findings suggest that Sida acuta possesses antibacterial properties that may be beneficial in treating ulcer-related H. pylori infections.
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| format | Article |
| id | doaj-art-d0bd0bec9dd6451b83a55fb0eb8643d2 |
| institution | Kabale University |
| issn | 2616-0668 2814-1822 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Umaru Musa Yar'adua University, Katsina, Nigeria |
| record_format | Article |
| series | UMYU Journal of Microbiology Research |
| spelling | doaj-art-d0bd0bec9dd6451b83a55fb0eb8643d22025-02-04T12:22:01ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222024-12-019210.47430/ujmr.2492.018Phytochemical Profiling, GC-MS Analysis and Antibacterial Activity of Sida acuta leave Extracts Against Helicobacter pyloriKamoldeen Ajijolakewu0Ayobami Elias Ajadi1S A Ayoola2Sheriffdeen Issa Bale3Taiwo Ayodeji Sorunke4Saheed Abayomi Onifade5Tomisin Oyawoye6Department of Microbiology, Faculty of Life Sciences, University of Ilorin, Kwara State, NigeriaDepartment of Microbiology; 3Department of Biotechnology and Molecular Biology; Federal University of Health Sciences, Ila Orangun, Osun State, NigeriaBioresources Development Centre, National Biotechnology Development Agency, Ogbomosho, Oyo State, NigeriaDepartment of Microbiology; 3Department of Biotechnology and Molecular Biology; Federal University of Health Sciences, Ila Orangun, Osun State, NigeriaDepartment of Microbiology; 3Department of Biotechnology and Molecular Biology; Federal University of Health Sciences, Ila Orangun, Osun State, NigeriaDepartment of Biotechnology and Molecular Biology; Federal University of Health Sciences, Ila Orangun, Osun State, NigeriaDepartment of Microbiology; Federal University of Health Sciences, Ila Orangun, Osun State, Nigeria Study’s Excerpt: • The antibacterial potential of Sida acuta against Helicobacter pylori is assessed. • Phytochemical profiling, GC-MS analysis, and antibacterial assays were integrated to determine the bioactive compounds in the plant. • The findings reveals that Sida acuta has antibacterial properties against ulcer-related H. pylori. Full Abstract: Helicobacter pylori is a major cause of ulcers, and increasing resistance to standard antibiotics underscores the need for alternative treatments. Sida acuta, a plant with medicinal properties, was examined in this study for its potential antibacterial effects. This research assessed the phytochemical profile, performed Gas Chromatography-Mass Spectrometry (GC-MS) analysis, and evaluated the antibacterial efficacy of Sida acuta extracts against H. pylori. Leaves of S. acuta were extracted using hexane, ethanol, and water, and their phytochemical constituents were analyzed through established qualitative and quantitative methods. The antibacterial activity of the leaf extracts was tested against H. pylori via agar well diffusion and broth dilution using various extract concentrations. GC-MS analysis was conducted to identify the bioactive compounds in each extract. Phytochemical screening revealed the presence of alkaloids, saponins, glycosides, tannins, flavonoids, triterpenoids, steroids, and phenols in the extracts. Aqueous extracts exhibited the largest inhibition zones (20.23±0.16 mm) at 500 mg/mL, followed by ethanol (18.25±0.25 mm) and hexane (16.35±0.25 mm) extracts. Minimum inhibitory concentrations (MIC) were determined as 62.5 mg/mL for ethanol, 125 mg/mL for water, and 250 mg/mL for hexane extracts. Bactericidal effects were observed with the ethanol and aqueous extracts at 250 mg/mL and 500 mg/mL, respectively, while hexane showed no bactericidal activity at tested concentrations. GC-MS identified 27 compounds in the ethanol extract, 20 in the aqueous extract, and 25 in the hexane extract. These findings suggest that Sida acuta possesses antibacterial properties that may be beneficial in treating ulcer-related H. pylori infections. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/626Antibacterial activityGC-MS analysisPhytochemical profilingSida acutaHelicobacter pylori |
| spellingShingle | Kamoldeen Ajijolakewu Ayobami Elias Ajadi S A Ayoola Sheriffdeen Issa Bale Taiwo Ayodeji Sorunke Saheed Abayomi Onifade Tomisin Oyawoye Phytochemical Profiling, GC-MS Analysis and Antibacterial Activity of Sida acuta leave Extracts Against Helicobacter pylori UMYU Journal of Microbiology Research Antibacterial activity GC-MS analysis Phytochemical profiling Sida acuta Helicobacter pylori |
| title | Phytochemical Profiling, GC-MS Analysis and Antibacterial Activity of Sida acuta leave Extracts Against Helicobacter pylori |
| title_full | Phytochemical Profiling, GC-MS Analysis and Antibacterial Activity of Sida acuta leave Extracts Against Helicobacter pylori |
| title_fullStr | Phytochemical Profiling, GC-MS Analysis and Antibacterial Activity of Sida acuta leave Extracts Against Helicobacter pylori |
| title_full_unstemmed | Phytochemical Profiling, GC-MS Analysis and Antibacterial Activity of Sida acuta leave Extracts Against Helicobacter pylori |
| title_short | Phytochemical Profiling, GC-MS Analysis and Antibacterial Activity of Sida acuta leave Extracts Against Helicobacter pylori |
| title_sort | phytochemical profiling gc ms analysis and antibacterial activity of sida acuta leave extracts against helicobacter pylori |
| topic | Antibacterial activity GC-MS analysis Phytochemical profiling Sida acuta Helicobacter pylori |
| url | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/626 |
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