Differentiation of <i>Amaranthus</i> Species and Estimation of Their Polyphenolic Compounds and Antioxidant Potential Using Near-Infrared Spectroscopy
<i>Amaranthus</i> species are rich in protein, fiber, minerals, and other nutrients and have various health benefits. The genus is taxonomically difficult due to the high phenotypic plasticity and the spontaneous interspecies introgression and hybridization between species. The purpose o...
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2024-11-01
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| author | Svetoslava Terzieva Neli Grozeva Milena Tzanova Petya Veleva Mariya Gerdzhikova Stefka Atanassova |
| author_facet | Svetoslava Terzieva Neli Grozeva Milena Tzanova Petya Veleva Mariya Gerdzhikova Stefka Atanassova |
| author_sort | Svetoslava Terzieva |
| collection | DOAJ |
| description | <i>Amaranthus</i> species are rich in protein, fiber, minerals, and other nutrients and have various health benefits. The genus is taxonomically difficult due to the high phenotypic plasticity and the spontaneous interspecies introgression and hybridization between species. The purpose of this study is to evaluate the possibilities of near-infrared spectroscopy (NIRS) for the taxonomic differentiation of some of the species common in Bulgaria and estimate their polyphenolic compounds. Tested samples were collected from six Bulgarian floristic regions: <i>Amaranthus albus</i> L., <i>A. blitum</i> L., <i>A. deflexus</i> L., <i>A. hybridus</i> L., and <i>A. retroflexus</i> L. were studied. The NIR spectra of dried and ground leaf and stalk samples were measured by NIRQuest 512 (region 900–1700 nm) using a fiber-optic probe. Soft independent modeling of class analogy (SIMCA) was used to develop the classification models and PLS regression for the quantitative determination of their polyphenolic compounds and antioxidant potential. There were statistically significant differences in the measured values of polyphenolic compounds and antioxidant potential among the tested species. NIRS allowed an accurate determination of these parameters. The performance of developed SIMCA models for the discrimination of species was very high. The precision of determination varied from 98.2 to 100%, and the total accuracy was 98.34%. The results show successful differentiation of the taxonomic species. |
| format | Article |
| id | doaj-art-d0bbdfcedfee4485bc014557b1897c3a |
| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-11-01 |
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| series | Plants |
| spelling | doaj-art-d0bbdfcedfee4485bc014557b1897c3a2025-08-20T02:50:40ZengMDPI AGPlants2223-77472024-11-011323337010.3390/plants13233370Differentiation of <i>Amaranthus</i> Species and Estimation of Their Polyphenolic Compounds and Antioxidant Potential Using Near-Infrared SpectroscopySvetoslava Terzieva0Neli Grozeva1Milena Tzanova2Petya Veleva3Mariya Gerdzhikova4Stefka Atanassova5Faculty of Agriculture, Students’ Campus, Trakia University, 6000 Stara Zagora, BulgariaFaculty of Agriculture, Students’ Campus, Trakia University, 6000 Stara Zagora, BulgariaFaculty of Agriculture, Students’ Campus, Trakia University, 6000 Stara Zagora, BulgariaFaculty of Agriculture, Students’ Campus, Trakia University, 6000 Stara Zagora, BulgariaFaculty of Agriculture, Students’ Campus, Trakia University, 6000 Stara Zagora, BulgariaFaculty of Agriculture, Students’ Campus, Trakia University, 6000 Stara Zagora, Bulgaria<i>Amaranthus</i> species are rich in protein, fiber, minerals, and other nutrients and have various health benefits. The genus is taxonomically difficult due to the high phenotypic plasticity and the spontaneous interspecies introgression and hybridization between species. The purpose of this study is to evaluate the possibilities of near-infrared spectroscopy (NIRS) for the taxonomic differentiation of some of the species common in Bulgaria and estimate their polyphenolic compounds. Tested samples were collected from six Bulgarian floristic regions: <i>Amaranthus albus</i> L., <i>A. blitum</i> L., <i>A. deflexus</i> L., <i>A. hybridus</i> L., and <i>A. retroflexus</i> L. were studied. The NIR spectra of dried and ground leaf and stalk samples were measured by NIRQuest 512 (region 900–1700 nm) using a fiber-optic probe. Soft independent modeling of class analogy (SIMCA) was used to develop the classification models and PLS regression for the quantitative determination of their polyphenolic compounds and antioxidant potential. There were statistically significant differences in the measured values of polyphenolic compounds and antioxidant potential among the tested species. NIRS allowed an accurate determination of these parameters. The performance of developed SIMCA models for the discrimination of species was very high. The precision of determination varied from 98.2 to 100%, and the total accuracy was 98.34%. The results show successful differentiation of the taxonomic species.https://www.mdpi.com/2223-7747/13/23/3370<i>Amaranthus albus</i> L.<i>Amaranthus blitum</i> L.<i>Amaranthus deflexus</i> L.<i>Amaranthus hybridus</i> L.<i>Amaranthus retroflexus</i> L.NIR spectroscopy |
| spellingShingle | Svetoslava Terzieva Neli Grozeva Milena Tzanova Petya Veleva Mariya Gerdzhikova Stefka Atanassova Differentiation of <i>Amaranthus</i> Species and Estimation of Their Polyphenolic Compounds and Antioxidant Potential Using Near-Infrared Spectroscopy Plants <i>Amaranthus albus</i> L. <i>Amaranthus blitum</i> L. <i>Amaranthus deflexus</i> L. <i>Amaranthus hybridus</i> L. <i>Amaranthus retroflexus</i> L. NIR spectroscopy |
| title | Differentiation of <i>Amaranthus</i> Species and Estimation of Their Polyphenolic Compounds and Antioxidant Potential Using Near-Infrared Spectroscopy |
| title_full | Differentiation of <i>Amaranthus</i> Species and Estimation of Their Polyphenolic Compounds and Antioxidant Potential Using Near-Infrared Spectroscopy |
| title_fullStr | Differentiation of <i>Amaranthus</i> Species and Estimation of Their Polyphenolic Compounds and Antioxidant Potential Using Near-Infrared Spectroscopy |
| title_full_unstemmed | Differentiation of <i>Amaranthus</i> Species and Estimation of Their Polyphenolic Compounds and Antioxidant Potential Using Near-Infrared Spectroscopy |
| title_short | Differentiation of <i>Amaranthus</i> Species and Estimation of Their Polyphenolic Compounds and Antioxidant Potential Using Near-Infrared Spectroscopy |
| title_sort | differentiation of i amaranthus i species and estimation of their polyphenolic compounds and antioxidant potential using near infrared spectroscopy |
| topic | <i>Amaranthus albus</i> L. <i>Amaranthus blitum</i> L. <i>Amaranthus deflexus</i> L. <i>Amaranthus hybridus</i> L. <i>Amaranthus retroflexus</i> L. NIR spectroscopy |
| url | https://www.mdpi.com/2223-7747/13/23/3370 |
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