Exploring molecular and modular insights into space ionizing radiation effects through heterogeneous gene regulatory networks

Abstract Space ionizing radiation is a major risk factor for astronauts, yet its molecular mechanisms remain poorly understood. This study employs an integrative approach to investigate the effects of space ionizing radiation on molecules, modules, biological functions, associated diseases, and pote...

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Main Authors: Mengqin Yuan, Tao Zhang, Haizhou Liu, Min Long, Quan Wang, Wei Jiang
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:npj Microgravity
Online Access:https://doi.org/10.1038/s41526-025-00508-6
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author Mengqin Yuan
Tao Zhang
Haizhou Liu
Min Long
Quan Wang
Wei Jiang
author_facet Mengqin Yuan
Tao Zhang
Haizhou Liu
Min Long
Quan Wang
Wei Jiang
author_sort Mengqin Yuan
collection DOAJ
description Abstract Space ionizing radiation is a major risk factor for astronauts, yet its molecular mechanisms remain poorly understood. This study employs an integrative approach to investigate the effects of space ionizing radiation on molecules, modules, biological functions, associated diseases, and potential therapeutic drugs. Using paired samples from five donors subjected to acute ex vivo 2Gy gamma-ray irradiation, we analyzed miRNA and gene expression profiles in human peripheral blood lymphocytes collected 24 h post-exposure, combined with heterogeneous network analysis, identifying 179 key molecules (23 transcription factors, 10 miRNAs, and 146 genes) and 5 key modules. Functional enrichment analysis revealed associations with processes such as cell cycle regulation, cytidine deamination, cell differentiation, viral carcinogenesis, and apoptosis. Radiation was also significantly linked to neoplasms and digestive system diseases. Furthermore, we predicted 20 potential therapeutic compounds, including small molecules (e.g., Navitoclax) and Traditional Chinese Medicine ingredients (e.g., Genistin, Saikosaponin D), which may alleviate radiation-induced damage such as pulmonary fibrosis and oxidative stress. These findings provide novel insights into the molecular mechanisms of space ionizing radiation and may contribute to developing effective strategies to protect astronaut health during space missions.
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spelling doaj-art-d0b32b6907e84f4e8baeb83142e23dca2025-08-20T04:03:06ZengNature Portfolionpj Microgravity2373-80652025-07-0111111210.1038/s41526-025-00508-6Exploring molecular and modular insights into space ionizing radiation effects through heterogeneous gene regulatory networksMengqin Yuan0Tao Zhang1Haizhou Liu2Min Long3Quan Wang4Wei Jiang5Fujian Provincial Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital, Fujian Medical UniversityDepartment of Biomedical Engineering, Nanjing University of Aeronautics and AstronauticsFujian Provincial Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital, Fujian Medical UniversityDepartment of Biomedical Engineering, Nanjing University of Aeronautics and AstronauticsDepartment of Biomedical Engineering, Nanjing University of Aeronautics and AstronauticsFujian Provincial Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital, Fujian Medical UniversityAbstract Space ionizing radiation is a major risk factor for astronauts, yet its molecular mechanisms remain poorly understood. This study employs an integrative approach to investigate the effects of space ionizing radiation on molecules, modules, biological functions, associated diseases, and potential therapeutic drugs. Using paired samples from five donors subjected to acute ex vivo 2Gy gamma-ray irradiation, we analyzed miRNA and gene expression profiles in human peripheral blood lymphocytes collected 24 h post-exposure, combined with heterogeneous network analysis, identifying 179 key molecules (23 transcription factors, 10 miRNAs, and 146 genes) and 5 key modules. Functional enrichment analysis revealed associations with processes such as cell cycle regulation, cytidine deamination, cell differentiation, viral carcinogenesis, and apoptosis. Radiation was also significantly linked to neoplasms and digestive system diseases. Furthermore, we predicted 20 potential therapeutic compounds, including small molecules (e.g., Navitoclax) and Traditional Chinese Medicine ingredients (e.g., Genistin, Saikosaponin D), which may alleviate radiation-induced damage such as pulmonary fibrosis and oxidative stress. These findings provide novel insights into the molecular mechanisms of space ionizing radiation and may contribute to developing effective strategies to protect astronaut health during space missions.https://doi.org/10.1038/s41526-025-00508-6
spellingShingle Mengqin Yuan
Tao Zhang
Haizhou Liu
Min Long
Quan Wang
Wei Jiang
Exploring molecular and modular insights into space ionizing radiation effects through heterogeneous gene regulatory networks
npj Microgravity
title Exploring molecular and modular insights into space ionizing radiation effects through heterogeneous gene regulatory networks
title_full Exploring molecular and modular insights into space ionizing radiation effects through heterogeneous gene regulatory networks
title_fullStr Exploring molecular and modular insights into space ionizing radiation effects through heterogeneous gene regulatory networks
title_full_unstemmed Exploring molecular and modular insights into space ionizing radiation effects through heterogeneous gene regulatory networks
title_short Exploring molecular and modular insights into space ionizing radiation effects through heterogeneous gene regulatory networks
title_sort exploring molecular and modular insights into space ionizing radiation effects through heterogeneous gene regulatory networks
url https://doi.org/10.1038/s41526-025-00508-6
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