Abnormal Expression of Peripheral Blood Leukocyte Surface Markers in Retinopathy of Prematurity Patients
Rui Guo,1 Fang Cheng,1 Xiang-Jie Meng,2,3 Jun-Mei Fan,4 Ya-Ling Lu,5 Xiao-Hui Ma,6 Xin Qiao,6 Jun-Hong Li7 1Department of Ophthalmology, Children’s Hospital of Shanxi and Women Health Center of Shanxi, Affiliated to Shanxi Medical University, Taiyuan, 030000, People’s Republic of China; 2Department...
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Dove Medical Press
2025-02-01
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| Series: | Journal of Multidisciplinary Healthcare |
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| author | Guo R Cheng F Meng XJ Fan JM Lu YL Ma XH Qiao X Li JH |
| author_facet | Guo R Cheng F Meng XJ Fan JM Lu YL Ma XH Qiao X Li JH |
| author_sort | Guo R |
| collection | DOAJ |
| description | Rui Guo,1 Fang Cheng,1 Xiang-Jie Meng,2,3 Jun-Mei Fan,4 Ya-Ling Lu,5 Xiao-Hui Ma,6 Xin Qiao,6 Jun-Hong Li7 1Department of Ophthalmology, Children’s Hospital of Shanxi and Women Health Center of Shanxi, Affiliated to Shanxi Medical University, Taiyuan, 030000, People’s Republic of China; 2Department of Pediatric Stomatology, Shanxi Medical University School and Hospital of Stomatology, Taiyuan, 030001, People’s Republic of China; 3Department of Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi Medical University School and Hospital of Stomatology, Taiyuan, 030001, People’s Republic of China; 4Department of Reproductive Medicine Center, Children’s Hospital of Shanxi and Women Health Center of Shanxi, Affiliated to Shanxi Medical University, Taiyuan, 030000, People’s Republic of China; 5School of Basic Medicine, Shanxi Medical University, Taiyuan, 030001, People’s Republic of China; 6Department of Shanxi Province Key Laboratory of Ophthalmology, Shanxi Eye Hospital Affiliated to Shanxi Medical University, Taiyuan, 030002, People’s Republic of China; 7Department of Strabismus and Pediatric Ophthalmology, Shanxi Eye Hospital, Affiliated to Shanxi Medical University, Taiyuan, 030002, People’s Republic of ChinaCorrespondence: Jun-Hong Li, Department of Strabismus and Pediatric Ophthalmology, Shanxi Eye Hospital Affiliated to Shanxi Medical University, No. 100 Fudong Street, Taiyuan, 030002, People’s Republic of China, Tel +8613834131099, Email junhongliljh@126.comObjective: Our study compares immune cell profiles in preterm infants with and without severe ROP, identifying risk factors for its development to explore both immunological aspects and determinants of ROP in preterm infants.Methods: Infants born between January 2023 to December 2023 at the Children’s Hospital of Shanxi were enrolled in this study according to the inclusion criteria. Patients were divided into a test group or a control group based on the need for Type 1 ROP treatment. Baseline data for both groups were compared. Neutrophil, lymphocyte, and monocyte subsets in the peripheral blood were analyzed using immunophenotypic analysis via multicolor flow cytometry. This method allowed for the quantification of specific cell subset proportions.Results: A total of 2,110 preterm infants were screened for inclusion in the study. Multivariate logistic regression analysis identified gestational age below 28 weeks, birth weight less than 1,000 g, and neonatal sepsis as independent risk factors for severe ROP. Furthermore, flow cytometry analysis was performed on blood samples from 45 preterm patients. Comparative analysis revealed that the test group had a lower percentage of neutrophils and higher expression of cluster of differentiation 81 (CD81) compared to the control group. Additionally, the test group showed a higher percentage of lymphocytes and a greater proportion of Th17 cells than the control group.Conclusion: Preterm gestational age, low birth weight, and neonatal sepsis increase severe ROP risk. Elevated CD81 and Th17 levels suggest inflammation linked to neutrophils and lymphocytes.Keywords: flow cytometry, inflammatory response, lymphocytes, neutrophils, retinopathy of prematurity |
| format | Article |
| id | doaj-art-d0abacc8eb9e4dad8bc8dc7318a2d195 |
| institution | Kabale University |
| issn | 1178-2390 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Multidisciplinary Healthcare |
| spelling | doaj-art-d0abacc8eb9e4dad8bc8dc7318a2d1952025-02-09T16:10:20ZengDove Medical PressJournal of Multidisciplinary Healthcare1178-23902025-02-01Volume 1865166199985Abnormal Expression of Peripheral Blood Leukocyte Surface Markers in Retinopathy of Prematurity PatientsGuo RCheng FMeng XJFan JMLu YLMa XHQiao XLi JHRui Guo,1 Fang Cheng,1 Xiang-Jie Meng,2,3 Jun-Mei Fan,4 Ya-Ling Lu,5 Xiao-Hui Ma,6 Xin Qiao,6 Jun-Hong Li7 1Department of Ophthalmology, Children’s Hospital of Shanxi and Women Health Center of Shanxi, Affiliated to Shanxi Medical University, Taiyuan, 030000, People’s Republic of China; 2Department of Pediatric Stomatology, Shanxi Medical University School and Hospital of Stomatology, Taiyuan, 030001, People’s Republic of China; 3Department of Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi Medical University School and Hospital of Stomatology, Taiyuan, 030001, People’s Republic of China; 4Department of Reproductive Medicine Center, Children’s Hospital of Shanxi and Women Health Center of Shanxi, Affiliated to Shanxi Medical University, Taiyuan, 030000, People’s Republic of China; 5School of Basic Medicine, Shanxi Medical University, Taiyuan, 030001, People’s Republic of China; 6Department of Shanxi Province Key Laboratory of Ophthalmology, Shanxi Eye Hospital Affiliated to Shanxi Medical University, Taiyuan, 030002, People’s Republic of China; 7Department of Strabismus and Pediatric Ophthalmology, Shanxi Eye Hospital, Affiliated to Shanxi Medical University, Taiyuan, 030002, People’s Republic of ChinaCorrespondence: Jun-Hong Li, Department of Strabismus and Pediatric Ophthalmology, Shanxi Eye Hospital Affiliated to Shanxi Medical University, No. 100 Fudong Street, Taiyuan, 030002, People’s Republic of China, Tel +8613834131099, Email junhongliljh@126.comObjective: Our study compares immune cell profiles in preterm infants with and without severe ROP, identifying risk factors for its development to explore both immunological aspects and determinants of ROP in preterm infants.Methods: Infants born between January 2023 to December 2023 at the Children’s Hospital of Shanxi were enrolled in this study according to the inclusion criteria. Patients were divided into a test group or a control group based on the need for Type 1 ROP treatment. Baseline data for both groups were compared. Neutrophil, lymphocyte, and monocyte subsets in the peripheral blood were analyzed using immunophenotypic analysis via multicolor flow cytometry. This method allowed for the quantification of specific cell subset proportions.Results: A total of 2,110 preterm infants were screened for inclusion in the study. Multivariate logistic regression analysis identified gestational age below 28 weeks, birth weight less than 1,000 g, and neonatal sepsis as independent risk factors for severe ROP. Furthermore, flow cytometry analysis was performed on blood samples from 45 preterm patients. Comparative analysis revealed that the test group had a lower percentage of neutrophils and higher expression of cluster of differentiation 81 (CD81) compared to the control group. Additionally, the test group showed a higher percentage of lymphocytes and a greater proportion of Th17 cells than the control group.Conclusion: Preterm gestational age, low birth weight, and neonatal sepsis increase severe ROP risk. Elevated CD81 and Th17 levels suggest inflammation linked to neutrophils and lymphocytes.Keywords: flow cytometry, inflammatory response, lymphocytes, neutrophils, retinopathy of prematurityhttps://www.dovepress.com/abnormal-expression-of-peripheral-blood-leukocyte-surface-markers-in-r-peer-reviewed-fulltext-article-JMDHflow cytometryinflammatory responselymphocytesneutrophilsretinopathy of prematurity |
| spellingShingle | Guo R Cheng F Meng XJ Fan JM Lu YL Ma XH Qiao X Li JH Abnormal Expression of Peripheral Blood Leukocyte Surface Markers in Retinopathy of Prematurity Patients Journal of Multidisciplinary Healthcare flow cytometry inflammatory response lymphocytes neutrophils retinopathy of prematurity |
| title | Abnormal Expression of Peripheral Blood Leukocyte Surface Markers in Retinopathy of Prematurity Patients |
| title_full | Abnormal Expression of Peripheral Blood Leukocyte Surface Markers in Retinopathy of Prematurity Patients |
| title_fullStr | Abnormal Expression of Peripheral Blood Leukocyte Surface Markers in Retinopathy of Prematurity Patients |
| title_full_unstemmed | Abnormal Expression of Peripheral Blood Leukocyte Surface Markers in Retinopathy of Prematurity Patients |
| title_short | Abnormal Expression of Peripheral Blood Leukocyte Surface Markers in Retinopathy of Prematurity Patients |
| title_sort | abnormal expression of peripheral blood leukocyte surface markers in retinopathy of prematurity patients |
| topic | flow cytometry inflammatory response lymphocytes neutrophils retinopathy of prematurity |
| url | https://www.dovepress.com/abnormal-expression-of-peripheral-blood-leukocyte-surface-markers-in-r-peer-reviewed-fulltext-article-JMDH |
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