Postnatal Development of Microglia-Like Cells in Mouse Cochlea

Microglial cells are involved in surveillance and cleaning of the central nervous system. Recently, microglial-like cells (MLC) have been found in an adult cochlea and investigated for their role in cochlear inflammation. The presence and potential roles of MLCs during the development of the cochlea...

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Main Authors: Penghui Chen, Yongchuan Chai, Haijin Liu, Gen Li, Longhao Wang, Tao Yang, Hao Wu
Format: Article
Language:English
Published: Wiley 2018-01-01
Series:Neural Plasticity
Online Access:http://dx.doi.org/10.1155/2018/1970150
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author Penghui Chen
Yongchuan Chai
Haijin Liu
Gen Li
Longhao Wang
Tao Yang
Hao Wu
author_facet Penghui Chen
Yongchuan Chai
Haijin Liu
Gen Li
Longhao Wang
Tao Yang
Hao Wu
author_sort Penghui Chen
collection DOAJ
description Microglial cells are involved in surveillance and cleaning of the central nervous system. Recently, microglial-like cells (MLC) have been found in an adult cochlea and investigated for their role in cochlear inflammation. The presence and potential roles of MLCs during the development of the cochlea, however, remain unclear. In this study, immunostaining was performed using the MLC-specific marker IBA1 to characterize the presence, distribution, and morphology of MLCs in the developing cochlea. From P0 to P14, MLCs were present in a variety of cochlear regions including the modiolus, spiral lamina, spiral ganglion, spiral ligament, and the organ of Corti. Interestingly, the overall number of MLCs in a mouse cochlea steadily increased since P0, peaks at P5, then gradually decreased from P5 to P14. In the spiral ligament, the distribution of the MLCs trends to shift from the type I/II fibrocyte-rich regions to the type III/IV fibrocyte-rich regions during the course of cochlear development, accompanied by the morphological changes of MLCs from the amoeboid, activated form to the ramified, quiescent form. Our results suggested that MLCs experience drastic morphological and distributional changes during postnatal cochlear development, which may play a role in the maturing and remodeling of the cochlea.
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spelling doaj-art-d09e2529ec014aadb94b4b049aba5f3c2025-08-20T03:19:37ZengWileyNeural Plasticity2090-59041687-54432018-01-01201810.1155/2018/19701501970150Postnatal Development of Microglia-Like Cells in Mouse CochleaPenghui Chen0Yongchuan Chai1Haijin Liu2Gen Li3Longhao Wang4Tao Yang5Hao Wu6Department of Otorhinolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Otorhinolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Pediatric Surgery, The First Affiliated Hospital of Gannan Medical University, Jiangxi Province, ChinaDepartment of Otorhinolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Otorhinolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Otorhinolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Otorhinolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaMicroglial cells are involved in surveillance and cleaning of the central nervous system. Recently, microglial-like cells (MLC) have been found in an adult cochlea and investigated for their role in cochlear inflammation. The presence and potential roles of MLCs during the development of the cochlea, however, remain unclear. In this study, immunostaining was performed using the MLC-specific marker IBA1 to characterize the presence, distribution, and morphology of MLCs in the developing cochlea. From P0 to P14, MLCs were present in a variety of cochlear regions including the modiolus, spiral lamina, spiral ganglion, spiral ligament, and the organ of Corti. Interestingly, the overall number of MLCs in a mouse cochlea steadily increased since P0, peaks at P5, then gradually decreased from P5 to P14. In the spiral ligament, the distribution of the MLCs trends to shift from the type I/II fibrocyte-rich regions to the type III/IV fibrocyte-rich regions during the course of cochlear development, accompanied by the morphological changes of MLCs from the amoeboid, activated form to the ramified, quiescent form. Our results suggested that MLCs experience drastic morphological and distributional changes during postnatal cochlear development, which may play a role in the maturing and remodeling of the cochlea.http://dx.doi.org/10.1155/2018/1970150
spellingShingle Penghui Chen
Yongchuan Chai
Haijin Liu
Gen Li
Longhao Wang
Tao Yang
Hao Wu
Postnatal Development of Microglia-Like Cells in Mouse Cochlea
Neural Plasticity
title Postnatal Development of Microglia-Like Cells in Mouse Cochlea
title_full Postnatal Development of Microglia-Like Cells in Mouse Cochlea
title_fullStr Postnatal Development of Microglia-Like Cells in Mouse Cochlea
title_full_unstemmed Postnatal Development of Microglia-Like Cells in Mouse Cochlea
title_short Postnatal Development of Microglia-Like Cells in Mouse Cochlea
title_sort postnatal development of microglia like cells in mouse cochlea
url http://dx.doi.org/10.1155/2018/1970150
work_keys_str_mv AT penghuichen postnataldevelopmentofmicroglialikecellsinmousecochlea
AT yongchuanchai postnataldevelopmentofmicroglialikecellsinmousecochlea
AT haijinliu postnataldevelopmentofmicroglialikecellsinmousecochlea
AT genli postnataldevelopmentofmicroglialikecellsinmousecochlea
AT longhaowang postnataldevelopmentofmicroglialikecellsinmousecochlea
AT taoyang postnataldevelopmentofmicroglialikecellsinmousecochlea
AT haowu postnataldevelopmentofmicroglialikecellsinmousecochlea