Development and validation of a new reverse-phase high-performance liquid chromatography (RP-HPLC) method for the quantifications of tolperisone loaded in liposomes
Tolperisone (TOL) is a muscle relaxant that tends to undergo uncontrollable degradation in the intestinal medium, leading to low oral bioavailability and the generation of genotoxic degradants. This study aimed to assess the possibility of using liposomes as an alternative carrier system that can al...
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Elsevier
2025-03-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715625000815 |
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| author | Rawan N. AlKaraki Rula R. Haddadin Shahed K. Tarawneh Marwa A.M. Alimari Rehan M. Alkasasbeh Tebat Al-ferdous E. Shamaileh Mustafa S. Almohtaseb Malek G. Hajaya |
| author_facet | Rawan N. AlKaraki Rula R. Haddadin Shahed K. Tarawneh Marwa A.M. Alimari Rehan M. Alkasasbeh Tebat Al-ferdous E. Shamaileh Mustafa S. Almohtaseb Malek G. Hajaya |
| author_sort | Rawan N. AlKaraki |
| collection | DOAJ |
| description | Tolperisone (TOL) is a muscle relaxant that tends to undergo uncontrollable degradation in the intestinal medium, leading to low oral bioavailability and the generation of genotoxic degradants. This study aimed to assess the possibility of using liposomes as an alternative carrier system that can alleviate the uncontrolled degradation of TOL, and to develop a reverse-phase, high performance liquid chromatography (RP-HPLC) method for the detection and quantification of TOL in liposomes. Effective isocratic chromatographic separation of TOL was accomplished using Sunniest C-18 column, acetonitrile: methanol: water (60:20:20) mixed with 5.5 v/v% triethylamine as a mobile phase. The flow rate of the mobile phase was 0.6 mL/min, column temperature was 35 ± 0.2 °C, and the injection volume was 10 μL. TOL retention time was 6.93 min, at a wavelength of 251 nm. The developed method was found to be specific, accurate (>98 %), precise (%RSD < 2 %), and reliable for TOL detection and quantification in the presence of liposomes matrix. The method's applicability was demonstrated by accurately quantifying TOL content in a commercial pharmaceutical formulation. The validated RP-HPLC method was used to demonstrate the suitability of liposomes as a carrier for TOL by evaluating its entrapment efficiency (14.76 %), along with achieving a slower drug in-vitro cumulative percentage release in the targeted media (compared to free TOL solution) from the liposomes. |
| format | Article |
| id | doaj-art-d09c06e766dc40c69b3ea34cc56d3a10 |
| institution | Kabale University |
| issn | 2211-7156 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj-art-d09c06e766dc40c69b3ea34cc56d3a102025-02-09T05:00:06ZengElsevierResults in Chemistry2211-71562025-03-0114102098Development and validation of a new reverse-phase high-performance liquid chromatography (RP-HPLC) method for the quantifications of tolperisone loaded in liposomesRawan N. AlKaraki0Rula R. Haddadin1Shahed K. Tarawneh2Marwa A.M. Alimari3Rehan M. Alkasasbeh4Tebat Al-ferdous E. Shamaileh5Mustafa S. Almohtaseb6Malek G. Hajaya7The Department of pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Mutah University, Alkarak 61710, Jordan; Corresponding author.The Department of pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Mutah University, Alkarak 61710, JordanThe Department of pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Mutah University, Alkarak 61710, JordanDepartment of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, The University of Jordan, Amman 11942, JordanThe Department of pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Mutah University, Alkarak 61710, JordanThe Department of pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Mutah University, Alkarak 61710, JordanThe Department of pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Mutah University, Alkarak 61710, JordanDepartment of Civil Engineering, Tafila Technical University, Tafila 66110, JordanTolperisone (TOL) is a muscle relaxant that tends to undergo uncontrollable degradation in the intestinal medium, leading to low oral bioavailability and the generation of genotoxic degradants. This study aimed to assess the possibility of using liposomes as an alternative carrier system that can alleviate the uncontrolled degradation of TOL, and to develop a reverse-phase, high performance liquid chromatography (RP-HPLC) method for the detection and quantification of TOL in liposomes. Effective isocratic chromatographic separation of TOL was accomplished using Sunniest C-18 column, acetonitrile: methanol: water (60:20:20) mixed with 5.5 v/v% triethylamine as a mobile phase. The flow rate of the mobile phase was 0.6 mL/min, column temperature was 35 ± 0.2 °C, and the injection volume was 10 μL. TOL retention time was 6.93 min, at a wavelength of 251 nm. The developed method was found to be specific, accurate (>98 %), precise (%RSD < 2 %), and reliable for TOL detection and quantification in the presence of liposomes matrix. The method's applicability was demonstrated by accurately quantifying TOL content in a commercial pharmaceutical formulation. The validated RP-HPLC method was used to demonstrate the suitability of liposomes as a carrier for TOL by evaluating its entrapment efficiency (14.76 %), along with achieving a slower drug in-vitro cumulative percentage release in the targeted media (compared to free TOL solution) from the liposomes.http://www.sciencedirect.com/science/article/pii/S2211715625000815Liposomes-loaded TolperisoneDelivery systemRP-HPLC method validationEntrapment efficiencyIn vitro release |
| spellingShingle | Rawan N. AlKaraki Rula R. Haddadin Shahed K. Tarawneh Marwa A.M. Alimari Rehan M. Alkasasbeh Tebat Al-ferdous E. Shamaileh Mustafa S. Almohtaseb Malek G. Hajaya Development and validation of a new reverse-phase high-performance liquid chromatography (RP-HPLC) method for the quantifications of tolperisone loaded in liposomes Results in Chemistry Liposomes-loaded Tolperisone Delivery system RP-HPLC method validation Entrapment efficiency In vitro release |
| title | Development and validation of a new reverse-phase high-performance liquid chromatography (RP-HPLC) method for the quantifications of tolperisone loaded in liposomes |
| title_full | Development and validation of a new reverse-phase high-performance liquid chromatography (RP-HPLC) method for the quantifications of tolperisone loaded in liposomes |
| title_fullStr | Development and validation of a new reverse-phase high-performance liquid chromatography (RP-HPLC) method for the quantifications of tolperisone loaded in liposomes |
| title_full_unstemmed | Development and validation of a new reverse-phase high-performance liquid chromatography (RP-HPLC) method for the quantifications of tolperisone loaded in liposomes |
| title_short | Development and validation of a new reverse-phase high-performance liquid chromatography (RP-HPLC) method for the quantifications of tolperisone loaded in liposomes |
| title_sort | development and validation of a new reverse phase high performance liquid chromatography rp hplc method for the quantifications of tolperisone loaded in liposomes |
| topic | Liposomes-loaded Tolperisone Delivery system RP-HPLC method validation Entrapment efficiency In vitro release |
| url | http://www.sciencedirect.com/science/article/pii/S2211715625000815 |
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