MbbHLH93, a transcription factor associated with cold and drought tolerance in Malus baccata
The bHLH transcription factor is known to regulate cold signals and stress tolerance. In the present study, a new bHLH gene MbbHLH93, located in the nucleus, was isolated from Malus baccata, whose up-regulated expression were strongly induced by cold and drought treatment, and MbbHLH93-overexpressed...
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| Language: | English |
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Maximum Academic Press
2024-01-01
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| Series: | Fruit Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/frures-0024-0032 |
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| author | Lihua Zhang Yu Xu Long Lv Tianhe Wang Wanda Liu Xingguo Li Wenhui Li Junwei Huo Deguo Han |
| author_facet | Lihua Zhang Yu Xu Long Lv Tianhe Wang Wanda Liu Xingguo Li Wenhui Li Junwei Huo Deguo Han |
| author_sort | Lihua Zhang |
| collection | DOAJ |
| description | The bHLH transcription factor is known to regulate cold signals and stress tolerance. In the present study, a new bHLH gene MbbHLH93, located in the nucleus, was isolated from Malus baccata, whose up-regulated expression were strongly induced by cold and drought treatment, and MbbHLH93-overexpressed heterologous lettuce plants displayed cold and drought stress-tolerant phenotypes. Determination of physiological and biochemical indexes associated with abiotic stress responses showed that overexpression of MbbHLH93 increased the activities of antioxidant enzymes superoxide dismutase, peroxidase, and catalase in lettuce plants treated with cold and drought stress, and decreased the contents of H2O2, O2·−, and malondialdehyde, which contributed to reducing cell membrane lipid peroxidation. Meanwhile, the accumulation of proline in transgenic plant cells increased, regulating cell osmotic pressure. Furthermore, quantitative expression analysis indicated that overexpression of MbbHLH93 improved the expression levels of LsCBFs, which were positive functional genes in response to cold and drought stress, enhancing plant tolerance. This research demonstrates that the MbbHLH93 is a key regulator in plant tolerance to cold and drought stresses, providing new knowledge for plant tolerance regulation. |
| format | Article |
| id | doaj-art-d097fde1e0e2469f85bedd9a863fc49a |
| institution | OA Journals |
| issn | 2769-4615 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Fruit Research |
| spelling | doaj-art-d097fde1e0e2469f85bedd9a863fc49a2025-08-20T02:27:11ZengMaximum Academic PressFruit Research2769-46152024-01-014111110.48130/frures-0024-0032frures-0024-0032MbbHLH93, a transcription factor associated with cold and drought tolerance in Malus baccataLihua Zhang0Yu Xu1Long Lv2Tianhe Wang3Wanda Liu4Xingguo Li5Wenhui Li6Junwei Huo7Deguo Han8The Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, Heilongjiang, ChinaThe Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, Heilongjiang, ChinaThe Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, Heilongjiang, ChinaHorticulture Branch of Heilongjiang Academy of Agricultural Sciences, Harbin 150040, Heilongjiang, ChinaHorticulture Branch of Heilongjiang Academy of Agricultural Sciences, Harbin 150040, Heilongjiang, ChinaThe Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, Heilongjiang, ChinaThe Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, Heilongjiang, ChinaThe Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, Heilongjiang, ChinaThe Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, Heilongjiang, ChinaThe bHLH transcription factor is known to regulate cold signals and stress tolerance. In the present study, a new bHLH gene MbbHLH93, located in the nucleus, was isolated from Malus baccata, whose up-regulated expression were strongly induced by cold and drought treatment, and MbbHLH93-overexpressed heterologous lettuce plants displayed cold and drought stress-tolerant phenotypes. Determination of physiological and biochemical indexes associated with abiotic stress responses showed that overexpression of MbbHLH93 increased the activities of antioxidant enzymes superoxide dismutase, peroxidase, and catalase in lettuce plants treated with cold and drought stress, and decreased the contents of H2O2, O2·−, and malondialdehyde, which contributed to reducing cell membrane lipid peroxidation. Meanwhile, the accumulation of proline in transgenic plant cells increased, regulating cell osmotic pressure. Furthermore, quantitative expression analysis indicated that overexpression of MbbHLH93 improved the expression levels of LsCBFs, which were positive functional genes in response to cold and drought stress, enhancing plant tolerance. This research demonstrates that the MbbHLH93 is a key regulator in plant tolerance to cold and drought stresses, providing new knowledge for plant tolerance regulation.https://www.maxapress.com/article/doi/10.48130/frures-0024-0032malus baccatambbhlh93cold and drought stresstranscriptional regulation |
| spellingShingle | Lihua Zhang Yu Xu Long Lv Tianhe Wang Wanda Liu Xingguo Li Wenhui Li Junwei Huo Deguo Han MbbHLH93, a transcription factor associated with cold and drought tolerance in Malus baccata Fruit Research malus baccata mbbhlh93 cold and drought stress transcriptional regulation |
| title | MbbHLH93, a transcription factor associated with cold and drought tolerance in Malus baccata |
| title_full | MbbHLH93, a transcription factor associated with cold and drought tolerance in Malus baccata |
| title_fullStr | MbbHLH93, a transcription factor associated with cold and drought tolerance in Malus baccata |
| title_full_unstemmed | MbbHLH93, a transcription factor associated with cold and drought tolerance in Malus baccata |
| title_short | MbbHLH93, a transcription factor associated with cold and drought tolerance in Malus baccata |
| title_sort | mbbhlh93 a transcription factor associated with cold and drought tolerance in malus baccata |
| topic | malus baccata mbbhlh93 cold and drought stress transcriptional regulation |
| url | https://www.maxapress.com/article/doi/10.48130/frures-0024-0032 |
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