Genome-Wide Identification and Expression Analysis of PkNRT Gene Family in Korean Pine (<i>Pinus koraiensis</i>)
The utilization of nitrogen (N) is crucial for the optimal growth and development of plants. As the dominant form of nitrogen in temperate soil, nitrate (NO<sub>3</sub><sup>−</sup>) is absorbed from the soil and redistributed to other organs through NO<sub>3</sub>...
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2025-01-01
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| author | Xinyu Zhao Haibo Wu Boyang Li Pengyang Wang Peng Zhang Hailong Shen Jianfei Yang |
| author_facet | Xinyu Zhao Haibo Wu Boyang Li Pengyang Wang Peng Zhang Hailong Shen Jianfei Yang |
| author_sort | Xinyu Zhao |
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| description | The utilization of nitrogen (N) is crucial for the optimal growth and development of plants. As the dominant form of nitrogen in temperate soil, nitrate (NO<sub>3</sub><sup>−</sup>) is absorbed from the soil and redistributed to other organs through NO<sub>3</sub><sup>−</sup> transporters (NRTs). Therefore, exploration of the role of NRTs in response to various NO<sub>3</sub><sup>−</sup> conditions is crucial for improving N utilization efficiency (NUE). Here, we present a comprehensive genome-wide analysis and characterization of the NRT gene family in Korean pine, an invaluable tree species cultivated extensively in northeastern China. A total of 76 <i>PkNRTs</i> were identified in Korean pine and further divided into three subfamilies (NRT1/NPF, NRT2, and NRT3) based on phylogenetic analysis. All <i>PkNRTs</i> were distributed on 11 chromosomes, with multiple tandem duplications observed. The tissue-specific expression analysis indicated that most <i>PkNRTs</i> showed differential expression in six vegetative tissues. Furthermore, a significantly greater number of lateral roots was observed in seedlings under nitrogen-deficient conditions, accompanied by an increase in both total root biomass and root length. The temporal expression profiles of 16 <i>PkNRTs</i> in seedling roots revealed that four <i>PkNRTs</i>, <i>PkNPF5.6</i>, <i>PkNPF5.13</i>, <i>PkNPF6.1</i>, and <i>PkNPF6.2</i>, exhibited significantly upregulated expression under the NO<sub>3</sub><sup>−</sup> deficiency condition, whereas robust induction was observed for <i>PkNPF1.1</i>, <i>PkNRT2.6</i>, and <i>PkNRT3.3</i> upon the NO<sub>3</sub><sup>−</sup> sufficiency condition. The expression patterns of the <i>PkNRTs</i> suggest their potential diverse roles as key participants in root NO<sub>3</sub><sup>−</sup> uptake under varying NO<sub>3</sub><sup>−</sup> conditions during root development. These findings would provide a theoretical foundation for further investigations into the functions of <i>PkNRTs</i> in Korean pine. |
| format | Article |
| id | doaj-art-d08ed1339b04491aab764c58e202e042 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-d08ed1339b04491aab764c58e202e0422025-01-24T13:46:53ZengMDPI AGPlants2223-77472025-01-0114223810.3390/plants14020238Genome-Wide Identification and Expression Analysis of PkNRT Gene Family in Korean Pine (<i>Pinus koraiensis</i>)Xinyu Zhao0Haibo Wu1Boyang Li2Pengyang Wang3Peng Zhang4Hailong Shen5Jianfei Yang6College of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaThe utilization of nitrogen (N) is crucial for the optimal growth and development of plants. As the dominant form of nitrogen in temperate soil, nitrate (NO<sub>3</sub><sup>−</sup>) is absorbed from the soil and redistributed to other organs through NO<sub>3</sub><sup>−</sup> transporters (NRTs). Therefore, exploration of the role of NRTs in response to various NO<sub>3</sub><sup>−</sup> conditions is crucial for improving N utilization efficiency (NUE). Here, we present a comprehensive genome-wide analysis and characterization of the NRT gene family in Korean pine, an invaluable tree species cultivated extensively in northeastern China. A total of 76 <i>PkNRTs</i> were identified in Korean pine and further divided into three subfamilies (NRT1/NPF, NRT2, and NRT3) based on phylogenetic analysis. All <i>PkNRTs</i> were distributed on 11 chromosomes, with multiple tandem duplications observed. The tissue-specific expression analysis indicated that most <i>PkNRTs</i> showed differential expression in six vegetative tissues. Furthermore, a significantly greater number of lateral roots was observed in seedlings under nitrogen-deficient conditions, accompanied by an increase in both total root biomass and root length. The temporal expression profiles of 16 <i>PkNRTs</i> in seedling roots revealed that four <i>PkNRTs</i>, <i>PkNPF5.6</i>, <i>PkNPF5.13</i>, <i>PkNPF6.1</i>, and <i>PkNPF6.2</i>, exhibited significantly upregulated expression under the NO<sub>3</sub><sup>−</sup> deficiency condition, whereas robust induction was observed for <i>PkNPF1.1</i>, <i>PkNRT2.6</i>, and <i>PkNRT3.3</i> upon the NO<sub>3</sub><sup>−</sup> sufficiency condition. The expression patterns of the <i>PkNRTs</i> suggest their potential diverse roles as key participants in root NO<sub>3</sub><sup>−</sup> uptake under varying NO<sub>3</sub><sup>−</sup> conditions during root development. These findings would provide a theoretical foundation for further investigations into the functions of <i>PkNRTs</i> in Korean pine.https://www.mdpi.com/2223-7747/14/2/238Korean pineNO<sub>3</sub><sup>−</sup> transportersNO<sub>3</sub><sup>−</sup> deficiencylateral root development |
| spellingShingle | Xinyu Zhao Haibo Wu Boyang Li Pengyang Wang Peng Zhang Hailong Shen Jianfei Yang Genome-Wide Identification and Expression Analysis of PkNRT Gene Family in Korean Pine (<i>Pinus koraiensis</i>) Plants Korean pine NO<sub>3</sub><sup>−</sup> transporters NO<sub>3</sub><sup>−</sup> deficiency lateral root development |
| title | Genome-Wide Identification and Expression Analysis of PkNRT Gene Family in Korean Pine (<i>Pinus koraiensis</i>) |
| title_full | Genome-Wide Identification and Expression Analysis of PkNRT Gene Family in Korean Pine (<i>Pinus koraiensis</i>) |
| title_fullStr | Genome-Wide Identification and Expression Analysis of PkNRT Gene Family in Korean Pine (<i>Pinus koraiensis</i>) |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of PkNRT Gene Family in Korean Pine (<i>Pinus koraiensis</i>) |
| title_short | Genome-Wide Identification and Expression Analysis of PkNRT Gene Family in Korean Pine (<i>Pinus koraiensis</i>) |
| title_sort | genome wide identification and expression analysis of pknrt gene family in korean pine i pinus koraiensis i |
| topic | Korean pine NO<sub>3</sub><sup>−</sup> transporters NO<sub>3</sub><sup>−</sup> deficiency lateral root development |
| url | https://www.mdpi.com/2223-7747/14/2/238 |
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