Pattern specification and immune response transcriptional signatures of pericardial and subcutaneous adipose tissue.
Cardiovascular disease (CVD) remains the leading cause of morbidity and mortality in the United States. Recent studies suggest that pericardial adipose tissue (PCAT) secretes inflammatory factors that contribute to the development of CVD. To better characterize the role of PCAT in the pathogenesis o...
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| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0026092&type=printable |
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| author | Frank H Lau Rahul C Deo Gregory Mowrer Joshua Caplin Tim Ahfeldt Adam Kaplan Leon Ptaszek Jennifer D Walker Bruce R Rosengard Chad A Cowan |
| author_facet | Frank H Lau Rahul C Deo Gregory Mowrer Joshua Caplin Tim Ahfeldt Adam Kaplan Leon Ptaszek Jennifer D Walker Bruce R Rosengard Chad A Cowan |
| author_sort | Frank H Lau |
| collection | DOAJ |
| description | Cardiovascular disease (CVD) remains the leading cause of morbidity and mortality in the United States. Recent studies suggest that pericardial adipose tissue (PCAT) secretes inflammatory factors that contribute to the development of CVD. To better characterize the role of PCAT in the pathogenesis of disease, we performed a large-scale unbiased analysis of the transcriptional differences between PCAT and subcutaneous adipose tissue, analysing 53 microarrays across 19 individuals. As it was unknown whether PCAT-secreted factors are produced by adipocytes or cells in the supporting stromal fraction, we also sought to identify differentially expressed genes in isolated pericardial adipocytes vs. isolated subcutaneous adipocytes. Using microarray analysis, we found that: 1) pericardial adipose tissue and isolated pericardial adipocytes both overexpress atherosclerosis-promoting chemokines and 2) pericardial and subcutaneous fat depots, as well as isolated pericardial adipocytes and subcutaneous adipocytes, express specific patterns of homeobox genes. In contrast, a core set of lipid processing genes showed no significant overlap with differentially expressed transcripts. These depot-specific homeobox signatures and transcriptional profiles strongly suggest different functional roles for the pericardial and subcutaneous adipose depots. Further characterization of these inter-depot differences should be a research priority. |
| format | Article |
| id | doaj-art-d08babaecd79469caa9afcd3fcef7d6a |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-d08babaecd79469caa9afcd3fcef7d6a2025-08-20T02:30:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01610e2609210.1371/journal.pone.0026092Pattern specification and immune response transcriptional signatures of pericardial and subcutaneous adipose tissue.Frank H LauRahul C DeoGregory MowrerJoshua CaplinTim AhfeldtAdam KaplanLeon PtaszekJennifer D WalkerBruce R RosengardChad A CowanCardiovascular disease (CVD) remains the leading cause of morbidity and mortality in the United States. Recent studies suggest that pericardial adipose tissue (PCAT) secretes inflammatory factors that contribute to the development of CVD. To better characterize the role of PCAT in the pathogenesis of disease, we performed a large-scale unbiased analysis of the transcriptional differences between PCAT and subcutaneous adipose tissue, analysing 53 microarrays across 19 individuals. As it was unknown whether PCAT-secreted factors are produced by adipocytes or cells in the supporting stromal fraction, we also sought to identify differentially expressed genes in isolated pericardial adipocytes vs. isolated subcutaneous adipocytes. Using microarray analysis, we found that: 1) pericardial adipose tissue and isolated pericardial adipocytes both overexpress atherosclerosis-promoting chemokines and 2) pericardial and subcutaneous fat depots, as well as isolated pericardial adipocytes and subcutaneous adipocytes, express specific patterns of homeobox genes. In contrast, a core set of lipid processing genes showed no significant overlap with differentially expressed transcripts. These depot-specific homeobox signatures and transcriptional profiles strongly suggest different functional roles for the pericardial and subcutaneous adipose depots. Further characterization of these inter-depot differences should be a research priority.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0026092&type=printable |
| spellingShingle | Frank H Lau Rahul C Deo Gregory Mowrer Joshua Caplin Tim Ahfeldt Adam Kaplan Leon Ptaszek Jennifer D Walker Bruce R Rosengard Chad A Cowan Pattern specification and immune response transcriptional signatures of pericardial and subcutaneous adipose tissue. PLoS ONE |
| title | Pattern specification and immune response transcriptional signatures of pericardial and subcutaneous adipose tissue. |
| title_full | Pattern specification and immune response transcriptional signatures of pericardial and subcutaneous adipose tissue. |
| title_fullStr | Pattern specification and immune response transcriptional signatures of pericardial and subcutaneous adipose tissue. |
| title_full_unstemmed | Pattern specification and immune response transcriptional signatures of pericardial and subcutaneous adipose tissue. |
| title_short | Pattern specification and immune response transcriptional signatures of pericardial and subcutaneous adipose tissue. |
| title_sort | pattern specification and immune response transcriptional signatures of pericardial and subcutaneous adipose tissue |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0026092&type=printable |
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