The effect of cacao bean extracts on the prevention of periodontal tissue breakdown in diabetic rats with orthodontic tooth movements
Objective: Proper management of orthodontic treatment in diabetic patients is essential due to the heightened risk of periodontal tissue breakdown associated with hyperglycemia. Cacao bean extracts (CBE) are known to reduce the inflammatory response and increase synthesis and angiogenesis in periodo...
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Elsevier
2024-07-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426824000915 |
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| author | Nuzulul Hikmah Mohamad Hidayat Nur Permatasari Aulanni'am Aulanni'am Achmad Rudijanto |
| author_facet | Nuzulul Hikmah Mohamad Hidayat Nur Permatasari Aulanni'am Aulanni'am Achmad Rudijanto |
| author_sort | Nuzulul Hikmah |
| collection | DOAJ |
| description | Objective: Proper management of orthodontic treatment in diabetic patients is essential due to the heightened risk of periodontal tissue breakdown associated with hyperglycemia. Cacao bean extracts (CBE) are known to reduce the inflammatory response and increase synthesis and angiogenesis in periodontitis. Therefore, this study aims to examine the effect of CBE on preventing periodontal tissue breakdown in diabetes with orthodontic force. Methods: A total of 25 Wistar rats were divided randomly into 5 groups, including non-diabetes, diabetes, diabetes cacao 125, 250, and 500 mg/kg BW. Diabetic rats were induced with the stratified dose of Streptozotocin, and a 30-g-force from orthodontic device was applied in all groups. Diabetes cacao group was given CBE for 7 days using a gastric probe. GCF samples were used to analyze the eNOS level through the ELISA method. NFκB, Collagen-1, and FGF-2 expression were then assessed using the immunohistochemical method, while the number of fibroblasts and blood vessels was observed using hematoxylin-eosin stained tissue. The data obtained were analyzed with one-way ANOVA and post hoc tests, with p < 0.05. Results: CBE at a dose of 250 mg/kg BW significantly increased eNOS level, Collagen-1, and FGF-2 expression, and the number of fibroblasts and blood vessels in diabetes groups. Meanwhile, the treatment decreased NFκB expression in diabetes groups (p < 0.05). Conclusion: This study proved that CBE increased periodontal ligament synthesis and angiogenesis and decreased inflammatory response, thereby preventing periodontal tissue breakdown in diabetic rat models with tooth movement. |
| format | Article |
| id | doaj-art-d07d0e902700489fa6662d0360b3353e |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-d07d0e902700489fa6662d0360b3353e2025-01-24T04:45:06ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682024-07-01144384389The effect of cacao bean extracts on the prevention of periodontal tissue breakdown in diabetic rats with orthodontic tooth movementsNuzulul Hikmah0Mohamad Hidayat1Nur Permatasari2Aulanni'am Aulanni'am3Achmad Rudijanto4Doctoral Program in Medical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia; Department of Dentistry-Biomedical Sciences, Oral and Maxillofacial Pathology, Faculty of Dentistry, Universitas Jember, Jember, Indonesia; Corresponding author. Department of Dentistry-Biomedical Sciences, Oral and Maxillofacial Pathology, Faculty of Dentistry, Universitas Jember, Jl. Kalimantan 37, Jember, 68121, Indonesia.Department of Orthopaedic, Saiful Anwar General Hospital, Faculty of Medicine, Universitas Brawijaya, Malang, IndonesiaDepartment of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang, IndonesiaDepartment of Internal Medicine, Saiful Anwar General Hospital, Faculty of Medicine, Universitas Brawijaya, Malang, IndonesiaObjective: Proper management of orthodontic treatment in diabetic patients is essential due to the heightened risk of periodontal tissue breakdown associated with hyperglycemia. Cacao bean extracts (CBE) are known to reduce the inflammatory response and increase synthesis and angiogenesis in periodontitis. Therefore, this study aims to examine the effect of CBE on preventing periodontal tissue breakdown in diabetes with orthodontic force. Methods: A total of 25 Wistar rats were divided randomly into 5 groups, including non-diabetes, diabetes, diabetes cacao 125, 250, and 500 mg/kg BW. Diabetic rats were induced with the stratified dose of Streptozotocin, and a 30-g-force from orthodontic device was applied in all groups. Diabetes cacao group was given CBE for 7 days using a gastric probe. GCF samples were used to analyze the eNOS level through the ELISA method. NFκB, Collagen-1, and FGF-2 expression were then assessed using the immunohistochemical method, while the number of fibroblasts and blood vessels was observed using hematoxylin-eosin stained tissue. The data obtained were analyzed with one-way ANOVA and post hoc tests, with p < 0.05. Results: CBE at a dose of 250 mg/kg BW significantly increased eNOS level, Collagen-1, and FGF-2 expression, and the number of fibroblasts and blood vessels in diabetes groups. Meanwhile, the treatment decreased NFκB expression in diabetes groups (p < 0.05). Conclusion: This study proved that CBE increased periodontal ligament synthesis and angiogenesis and decreased inflammatory response, thereby preventing periodontal tissue breakdown in diabetic rat models with tooth movement.http://www.sciencedirect.com/science/article/pii/S2212426824000915Cacao extractsDiabetesOrthodontic tooth movementsPeriodontal tissue |
| spellingShingle | Nuzulul Hikmah Mohamad Hidayat Nur Permatasari Aulanni'am Aulanni'am Achmad Rudijanto The effect of cacao bean extracts on the prevention of periodontal tissue breakdown in diabetic rats with orthodontic tooth movements Journal of Oral Biology and Craniofacial Research Cacao extracts Diabetes Orthodontic tooth movements Periodontal tissue |
| title | The effect of cacao bean extracts on the prevention of periodontal tissue breakdown in diabetic rats with orthodontic tooth movements |
| title_full | The effect of cacao bean extracts on the prevention of periodontal tissue breakdown in diabetic rats with orthodontic tooth movements |
| title_fullStr | The effect of cacao bean extracts on the prevention of periodontal tissue breakdown in diabetic rats with orthodontic tooth movements |
| title_full_unstemmed | The effect of cacao bean extracts on the prevention of periodontal tissue breakdown in diabetic rats with orthodontic tooth movements |
| title_short | The effect of cacao bean extracts on the prevention of periodontal tissue breakdown in diabetic rats with orthodontic tooth movements |
| title_sort | effect of cacao bean extracts on the prevention of periodontal tissue breakdown in diabetic rats with orthodontic tooth movements |
| topic | Cacao extracts Diabetes Orthodontic tooth movements Periodontal tissue |
| url | http://www.sciencedirect.com/science/article/pii/S2212426824000915 |
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