Genome-wide identification of a MADS-box transcription factor family and their expression during floral development in Coptis teeta wall
Abstract Background MADS-box transcription factors have been shown to be involved in multiple developmental processes, including the regulation of floral organ formation and pollen maturation. However, the role of the MADS-box gene family in floral development of the alpine plant species Coptis teet...
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BMC
2024-10-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-024-05714-0 |
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| author | Shao-Feng Duan Ji-Chen Yu Timothy Charles Baldwin Yuan Yuan Gui-Sheng Xiang Rui Cui Yan Zhao Xin-Chun Mo Ying-Chun Lu Yan-Li Liang |
| author_facet | Shao-Feng Duan Ji-Chen Yu Timothy Charles Baldwin Yuan Yuan Gui-Sheng Xiang Rui Cui Yan Zhao Xin-Chun Mo Ying-Chun Lu Yan-Li Liang |
| author_sort | Shao-Feng Duan |
| collection | DOAJ |
| description | Abstract Background MADS-box transcription factors have been shown to be involved in multiple developmental processes, including the regulation of floral organ formation and pollen maturation. However, the role of the MADS-box gene family in floral development of the alpine plant species Coptis teeta Wall, which is widely used in Traditional Chinese Medicine (TCM), is unknown. Results Sixty-six MADS-box genes were identified in the C. teeta genome. These genes were shown to be unevenly distributed throughout the genome of C. teeta. The majority of which (49) were classified as type I MADS-box genes and were further subdivided into four groups (Mα, Mβ, Mγ and Mδ). The remainder were identified as belonging to the type II MADS-box gene category. It was observed that four pairs of segmental and tandem duplication had occurred in the C. teeta MADS-box gene family, and that the ratios of Ka/Ks were less than 1, suggesting that these genes may have experienced purifying selection during evolution. Gene expression profiling analysis revealed that 38 MADS-box genes displayed differential expression patterns between the M and F floral phenotypes. Sixteen of these MADS-box genes were further verified by RT-qPCR. The 3D structure of each subfamily gene was predicted, further indicating that MADS-box genes of the same type possess structural similarities to the known template. Conclusions These data provide new insights into the molecular mechanism of dichogamy and herkogamy formation in C. teeta and establish a solid foundation for future studies of the MADS-box genes family in this medicinal plant species. |
| format | Article |
| id | doaj-art-d079bcf0df4b44b69124e1605d03b317 |
| institution | OA Journals |
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| language | English |
| publishDate | 2024-10-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj-art-d079bcf0df4b44b69124e1605d03b3172025-08-20T02:18:10ZengBMCBMC Plant Biology1471-22292024-10-0124111810.1186/s12870-024-05714-0Genome-wide identification of a MADS-box transcription factor family and their expression during floral development in Coptis teeta wallShao-Feng Duan0Ji-Chen Yu1Timothy Charles Baldwin2Yuan Yuan3Gui-Sheng Xiang4Rui Cui5Yan Zhao6Xin-Chun Mo7Ying-Chun Lu8Yan-Li Liang9College of Agronomy & Biotechnology, Yunnan Agricultural UniversityCollege of Agronomy & Biotechnology, Yunnan Agricultural UniversityFaculty of Science and Engineering, University of WolverhamptonCollege of Agronomy & Biotechnology, Yunnan Agricultural UniversityKey Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural UniversityYunnan Land and Resources Vocational CollegeCollege of Agronomy & Biotechnology, Yunnan Agricultural UniversityDepartment of Applied Technology, Lijiang Normal UniversityYunnan Agricultural University College of Education and Vocational Education, Yunnan Agricultural UniversityCollege of Agronomy & Biotechnology, Yunnan Agricultural UniversityAbstract Background MADS-box transcription factors have been shown to be involved in multiple developmental processes, including the regulation of floral organ formation and pollen maturation. However, the role of the MADS-box gene family in floral development of the alpine plant species Coptis teeta Wall, which is widely used in Traditional Chinese Medicine (TCM), is unknown. Results Sixty-six MADS-box genes were identified in the C. teeta genome. These genes were shown to be unevenly distributed throughout the genome of C. teeta. The majority of which (49) were classified as type I MADS-box genes and were further subdivided into four groups (Mα, Mβ, Mγ and Mδ). The remainder were identified as belonging to the type II MADS-box gene category. It was observed that four pairs of segmental and tandem duplication had occurred in the C. teeta MADS-box gene family, and that the ratios of Ka/Ks were less than 1, suggesting that these genes may have experienced purifying selection during evolution. Gene expression profiling analysis revealed that 38 MADS-box genes displayed differential expression patterns between the M and F floral phenotypes. Sixteen of these MADS-box genes were further verified by RT-qPCR. The 3D structure of each subfamily gene was predicted, further indicating that MADS-box genes of the same type possess structural similarities to the known template. Conclusions These data provide new insights into the molecular mechanism of dichogamy and herkogamy formation in C. teeta and establish a solid foundation for future studies of the MADS-box genes family in this medicinal plant species.https://doi.org/10.1186/s12870-024-05714-0Coptis teetaMADS-box transcription factorGenome-wideFloral developmentRT-qPCR |
| spellingShingle | Shao-Feng Duan Ji-Chen Yu Timothy Charles Baldwin Yuan Yuan Gui-Sheng Xiang Rui Cui Yan Zhao Xin-Chun Mo Ying-Chun Lu Yan-Li Liang Genome-wide identification of a MADS-box transcription factor family and their expression during floral development in Coptis teeta wall BMC Plant Biology Coptis teeta MADS-box transcription factor Genome-wide Floral development RT-qPCR |
| title | Genome-wide identification of a MADS-box transcription factor family and their expression during floral development in Coptis teeta wall |
| title_full | Genome-wide identification of a MADS-box transcription factor family and their expression during floral development in Coptis teeta wall |
| title_fullStr | Genome-wide identification of a MADS-box transcription factor family and their expression during floral development in Coptis teeta wall |
| title_full_unstemmed | Genome-wide identification of a MADS-box transcription factor family and their expression during floral development in Coptis teeta wall |
| title_short | Genome-wide identification of a MADS-box transcription factor family and their expression during floral development in Coptis teeta wall |
| title_sort | genome wide identification of a mads box transcription factor family and their expression during floral development in coptis teeta wall |
| topic | Coptis teeta MADS-box transcription factor Genome-wide Floral development RT-qPCR |
| url | https://doi.org/10.1186/s12870-024-05714-0 |
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