Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer
Purpose. To investigate the expression of TSP50 protein in human gastric cancers and its correlation with clinical/prognostic significance. Methods. Immunohistochemistry (IHC) analysis of TSP50 was performed on a tissue microarray (TMA) containing 334 primary gastric cancers. Western blot was carri...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2014/498246 |
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| author | Fang Liu Qinghua Cao Ni Liu Changzhao Li Changxuan You Chuanxin Liu Ling Xue Rongcheng Luo |
| author_facet | Fang Liu Qinghua Cao Ni Liu Changzhao Li Changxuan You Chuanxin Liu Ling Xue Rongcheng Luo |
| author_sort | Fang Liu |
| collection | DOAJ |
| description | Purpose. To investigate the expression of TSP50 protein in human gastric cancers and its correlation with clinical/prognostic significance.
Methods. Immunohistochemistry (IHC) analysis of TSP50 was performed on a tissue microarray (TMA) containing 334 primary gastric cancers. Western blot was carried out to confirm the expression of TSP50 in gastric cancers. Results. IHC analysis revealed high expression of TSP50 in 57.2% human gastric cancer samples (191 out of 334). However, it was poorly expressed in all of the 20 adjacent nontumor tissues. This was confirmed by western blot, which showed significantly higher levels of TSP50 expression in gastric cancer tissues than adjacent nontumor tissues. A significant association was found between high levels of TSP50 and clinicopathological characteristics including junior age at surgery (P=0.001), later TNM stage (P=0.000), and present lymph node metastases (P=0.003). The survival of gastric cancer patients with high expression of TSP50 was significantly shorter than that of the patients with low levels of TSP50 (P=0.021). Multivariate Cox regression analysis indicated that TSP50 overexpression was an independent prognostic factor for gastric cancer patients (P=0.017). Conclusions. Our data demonstrate that elevated TSP50 protein expression could be a potential predictor of poor prognosis in gastric cancer patients. |
| format | Article |
| id | doaj-art-d07267ead5994c4789b02c78a8feebd5 |
| institution | DOAJ |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-d07267ead5994c4789b02c78a8feebd52025-08-20T03:19:46ZengWileyGastroenterology Research and Practice1687-61211687-630X2014-01-01201410.1155/2014/498246498246Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric CancerFang Liu0Qinghua Cao1Ni Liu2Changzhao Li3Changxuan You4Chuanxin Liu5Ling Xue6Rongcheng Luo7Cancer Center, Southern Medical University, Guangzhou 510315, ChinaDepartment of Pathology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Pathology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Dermatology and Skin Diseases Research Center, University of Alabama at Birmingham, Birmingham, AL 35205, USADepartment of Medical Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, ChinaCancer Center, Southern Medical University, Guangzhou 510315, ChinaDepartment of Pathology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, ChinaCancer Center, Southern Medical University, Guangzhou 510315, ChinaPurpose. To investigate the expression of TSP50 protein in human gastric cancers and its correlation with clinical/prognostic significance. Methods. Immunohistochemistry (IHC) analysis of TSP50 was performed on a tissue microarray (TMA) containing 334 primary gastric cancers. Western blot was carried out to confirm the expression of TSP50 in gastric cancers. Results. IHC analysis revealed high expression of TSP50 in 57.2% human gastric cancer samples (191 out of 334). However, it was poorly expressed in all of the 20 adjacent nontumor tissues. This was confirmed by western blot, which showed significantly higher levels of TSP50 expression in gastric cancer tissues than adjacent nontumor tissues. A significant association was found between high levels of TSP50 and clinicopathological characteristics including junior age at surgery (P=0.001), later TNM stage (P=0.000), and present lymph node metastases (P=0.003). The survival of gastric cancer patients with high expression of TSP50 was significantly shorter than that of the patients with low levels of TSP50 (P=0.021). Multivariate Cox regression analysis indicated that TSP50 overexpression was an independent prognostic factor for gastric cancer patients (P=0.017). Conclusions. Our data demonstrate that elevated TSP50 protein expression could be a potential predictor of poor prognosis in gastric cancer patients.http://dx.doi.org/10.1155/2014/498246 |
| spellingShingle | Fang Liu Qinghua Cao Ni Liu Changzhao Li Changxuan You Chuanxin Liu Ling Xue Rongcheng Luo Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer Gastroenterology Research and Practice |
| title | Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer |
| title_full | Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer |
| title_fullStr | Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer |
| title_full_unstemmed | Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer |
| title_short | Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer |
| title_sort | overexpression of testes specific protease 50 tsp50 predicts poor prognosis in patients with gastric cancer |
| url | http://dx.doi.org/10.1155/2014/498246 |
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