Organic Solvents for Determination of Bacterial Endotoxin Index in Water-Insoluble Pharmaceutical Substances

Substances for manufacture of parenteral drugs require control by the «Bacterial Endotoxin» (BE) Index with the LAL-test. The aim of the study was to show possible applicability of organic solvents in BE determination by the gel-thromb test in case of water-insoluble pharmaceutical substances. The r...

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Main Authors: O. V. Shapovalova, G. V. Dolgova, N. P. Neugodova, G. A. Sapozhnikova
Format: Article
Language:Russian
Published: LLC "Publishing House OKI" 2020-05-01
Series:Антибиотики и Химиотерапия
Subjects:
Online Access:https://www.antibiotics-chemotherapy.ru/jour/article/view/509
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author O. V. Shapovalova
G. V. Dolgova
N. P. Neugodova
G. A. Sapozhnikova
author_facet O. V. Shapovalova
G. V. Dolgova
N. P. Neugodova
G. A. Sapozhnikova
author_sort O. V. Shapovalova
collection DOAJ
description Substances for manufacture of parenteral drugs require control by the «Bacterial Endotoxin» (BE) Index with the LAL-test. The aim of the study was to show possible applicability of organic solvents in BE determination by the gel-thromb test in case of water-insoluble pharmaceutical substances. The results confirmed that ethyl alcohol practically had no effect on the endotoxin activity. In the routine assays it is advisable to test solutions of the substances at the alcohol concentration of 6% below.
format Article
id doaj-art-d06ec879cd4f4622b9d93fb3fdf6e772
institution DOAJ
issn 0235-2990
language Russian
publishDate 2020-05-01
publisher LLC "Publishing House OKI"
record_format Article
series Антибиотики и Химиотерапия
spelling doaj-art-d06ec879cd4f4622b9d93fb3fdf6e7722025-08-20T02:49:36ZrusLLC "Publishing House OKI"Антибиотики и Химиотерапия0235-29902020-05-01589-104144508Organic Solvents for Determination of Bacterial Endotoxin Index in Water-Insoluble Pharmaceutical SubstancesO. V. Shapovalova0G. V. Dolgova1N. P. Neugodova2G. A. Sapozhnikova3Scientific Centre for Expertise of Medical Application ProductsScientific Centre for Expertise of Medical Application ProductsScientific Centre for Expertise of Medical Application ProductsScientific Centre for Expertise of Medical Application ProductsSubstances for manufacture of parenteral drugs require control by the «Bacterial Endotoxin» (BE) Index with the LAL-test. The aim of the study was to show possible applicability of organic solvents in BE determination by the gel-thromb test in case of water-insoluble pharmaceutical substances. The results confirmed that ethyl alcohol practically had no effect on the endotoxin activity. In the routine assays it is advisable to test solutions of the substances at the alcohol concentration of 6% below.https://www.antibiotics-chemotherapy.ru/jour/article/view/509water-insoluble pharmaceutical substancesbacterial endotoxinsethyl alcohol
spellingShingle O. V. Shapovalova
G. V. Dolgova
N. P. Neugodova
G. A. Sapozhnikova
Organic Solvents for Determination of Bacterial Endotoxin Index in Water-Insoluble Pharmaceutical Substances
Антибиотики и Химиотерапия
water-insoluble pharmaceutical substances
bacterial endotoxins
ethyl alcohol
title Organic Solvents for Determination of Bacterial Endotoxin Index in Water-Insoluble Pharmaceutical Substances
title_full Organic Solvents for Determination of Bacterial Endotoxin Index in Water-Insoluble Pharmaceutical Substances
title_fullStr Organic Solvents for Determination of Bacterial Endotoxin Index in Water-Insoluble Pharmaceutical Substances
title_full_unstemmed Organic Solvents for Determination of Bacterial Endotoxin Index in Water-Insoluble Pharmaceutical Substances
title_short Organic Solvents for Determination of Bacterial Endotoxin Index in Water-Insoluble Pharmaceutical Substances
title_sort organic solvents for determination of bacterial endotoxin index in water insoluble pharmaceutical substances
topic water-insoluble pharmaceutical substances
bacterial endotoxins
ethyl alcohol
url https://www.antibiotics-chemotherapy.ru/jour/article/view/509
work_keys_str_mv AT ovshapovalova organicsolventsfordeterminationofbacterialendotoxinindexinwaterinsolublepharmaceuticalsubstances
AT gvdolgova organicsolventsfordeterminationofbacterialendotoxinindexinwaterinsolublepharmaceuticalsubstances
AT npneugodova organicsolventsfordeterminationofbacterialendotoxinindexinwaterinsolublepharmaceuticalsubstances
AT gasapozhnikova organicsolventsfordeterminationofbacterialendotoxinindexinwaterinsolublepharmaceuticalsubstances