The impact of the physicochemical properties of calcium phosphate ceramics on biocompatibility and osteogenic differentiation of mesenchymal stem cells
Abstract Objective In this study we have focused on biocompatibility and osteoinductive capacity analysis of self-manufactured single-phase (HAP) and two-phase (HAP and β-ТСР) bioactive ceramics with various chemical modifications (Fig. 1). Results We demonstrate a reduction in solubility for all an...
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2024-10-01
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| Online Access: | https://doi.org/10.1186/s13104-024-06937-y |
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| author | Yuliia Skliarenko Volodymyr V. Kolomiiets Volodymyr V. Balatskyi Yuliia Galuza Oksana S. Koryak Larysa L. Macewicz Tetiana P. Ruban Sergey A. Firstov Nataliia. V. Ulianchych Oksana O. Piven |
| author_facet | Yuliia Skliarenko Volodymyr V. Kolomiiets Volodymyr V. Balatskyi Yuliia Galuza Oksana S. Koryak Larysa L. Macewicz Tetiana P. Ruban Sergey A. Firstov Nataliia. V. Ulianchych Oksana O. Piven |
| author_sort | Yuliia Skliarenko |
| collection | DOAJ |
| description | Abstract Objective In this study we have focused on biocompatibility and osteoinductive capacity analysis of self-manufactured single-phase (HAP) and two-phase (HAP and β-ТСР) bioactive ceramics with various chemical modifications (Fig. 1). Results We demonstrate a reduction in solubility for all analyzed composite after the treatment with H2O and H2O2, accompanied by an enhancement in adsorption activity. This modification also resulted in an increase in micro- and macroporosity, along with a rise in the open porosity. Adipose-derived mesenchymal stromal cells demonstrated excellent cell adhesion and survival when cultured with these ceramics. Calcium phosphate ceramics (H-500, HT-500, and HT-1 series) stimulated alkaline phosphatase expression, promoted calcium deposition, and enhanced osteopontin expression in ADSCs, independently inducing osteogenesis without additional osteogenic stimuli. These findings underscore the promising potential of HAP-based bioceramics for bone regeneration/reconstruction. Graphical Abstract |
| format | Article |
| id | doaj-art-d066e1f62f1f4676a37bb87f255a0166 |
| institution | Kabale University |
| issn | 1756-0500 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj-art-d066e1f62f1f4676a37bb87f255a01662025-02-09T12:09:52ZengBMCBMC Research Notes1756-05002024-10-011711710.1186/s13104-024-06937-yThe impact of the physicochemical properties of calcium phosphate ceramics on biocompatibility and osteogenic differentiation of mesenchymal stem cellsYuliia Skliarenko0Volodymyr V. Kolomiiets1Volodymyr V. Balatskyi2Yuliia Galuza3Oksana S. Koryak4Larysa L. Macewicz5Tetiana P. Ruban6Sergey A. Firstov7Nataliia. V. Ulianchych8Oksana O. Piven9Department of Internal Medicine, Medical Oncology & Pulmonology, VIII (AG Zender), University Clinic TübingenDepartment of Physics of Strength and Plasticity of Materials, Frantsevich Institute for Problems of Materials Science, National Academy of Science of UkraineLaboratory of Molecular Medical Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of SciencesDepartment of Human Genetics, Institute of Molecular Biology and Genetics, National Academy of Sciences of UkraineDepartment of phase transformations, Frantsevich Institute for Problems of Materials Science, National Academy of Science of UkraineDepartment of Human Genetics, Institute of Molecular Biology and Genetics, National Academy of Sciences of UkraineDepartment of Human Genetics, Institute of Molecular Biology and Genetics, National Academy of Sciences of UkraineDepartment of Physics of Strength and Plasticity of Materials, Frantsevich Institute for Problems of Materials Science, National Academy of Science of UkraineDepartment of Physics of Strength and Plasticity of Materials, Frantsevich Institute for Problems of Materials Science, National Academy of Science of UkraineDepartment of Human Genetics, Institute of Molecular Biology and Genetics, National Academy of Sciences of UkraineAbstract Objective In this study we have focused on biocompatibility and osteoinductive capacity analysis of self-manufactured single-phase (HAP) and two-phase (HAP and β-ТСР) bioactive ceramics with various chemical modifications (Fig. 1). Results We demonstrate a reduction in solubility for all analyzed composite after the treatment with H2O and H2O2, accompanied by an enhancement in adsorption activity. This modification also resulted in an increase in micro- and macroporosity, along with a rise in the open porosity. Adipose-derived mesenchymal stromal cells demonstrated excellent cell adhesion and survival when cultured with these ceramics. Calcium phosphate ceramics (H-500, HT-500, and HT-1 series) stimulated alkaline phosphatase expression, promoted calcium deposition, and enhanced osteopontin expression in ADSCs, independently inducing osteogenesis without additional osteogenic stimuli. These findings underscore the promising potential of HAP-based bioceramics for bone regeneration/reconstruction. Graphical Abstracthttps://doi.org/10.1186/s13104-024-06937-yHydroxyapatiteCalcium phosphate ceramicsStem cellsBiocompatibilityOsteogenesis |
| spellingShingle | Yuliia Skliarenko Volodymyr V. Kolomiiets Volodymyr V. Balatskyi Yuliia Galuza Oksana S. Koryak Larysa L. Macewicz Tetiana P. Ruban Sergey A. Firstov Nataliia. V. Ulianchych Oksana O. Piven The impact of the physicochemical properties of calcium phosphate ceramics on biocompatibility and osteogenic differentiation of mesenchymal stem cells BMC Research Notes Hydroxyapatite Calcium phosphate ceramics Stem cells Biocompatibility Osteogenesis |
| title | The impact of the physicochemical properties of calcium phosphate ceramics on biocompatibility and osteogenic differentiation of mesenchymal stem cells |
| title_full | The impact of the physicochemical properties of calcium phosphate ceramics on biocompatibility and osteogenic differentiation of mesenchymal stem cells |
| title_fullStr | The impact of the physicochemical properties of calcium phosphate ceramics on biocompatibility and osteogenic differentiation of mesenchymal stem cells |
| title_full_unstemmed | The impact of the physicochemical properties of calcium phosphate ceramics on biocompatibility and osteogenic differentiation of mesenchymal stem cells |
| title_short | The impact of the physicochemical properties of calcium phosphate ceramics on biocompatibility and osteogenic differentiation of mesenchymal stem cells |
| title_sort | impact of the physicochemical properties of calcium phosphate ceramics on biocompatibility and osteogenic differentiation of mesenchymal stem cells |
| topic | Hydroxyapatite Calcium phosphate ceramics Stem cells Biocompatibility Osteogenesis |
| url | https://doi.org/10.1186/s13104-024-06937-y |
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