The sucrose synthase gene family in blueberry (Vaccinium darrowii): functional insights into the role of VdSUS4 in salt stress tolerance
IntroductionThe sucrose synthase (SUS), a crucial enzyme in the sucrose metabolism, is encoded by a multigene family in plant kingdom. MethodsIn our study, we utilized bioinformatics tools to identify and characterize the members of the SUS gene family within the blueberry genome. Our analysis encom...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1581182/full |
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| author | Yanwen Wang Yanwen Wang Yanwen Wang Lei Yang Lei Yang Lei Yang Lei Yang Lei Yang Wenzhu Geng Wenzhu Geng Wenzhu Geng Hongxia Zhang Hongxia Zhang Hongxia Zhang Hongxia Zhang Hongxia Zhang Houjun Zhou Houjun Zhou Houjun Zhou Houjun Zhou Houjun Zhou |
| author_facet | Yanwen Wang Yanwen Wang Yanwen Wang Lei Yang Lei Yang Lei Yang Lei Yang Lei Yang Wenzhu Geng Wenzhu Geng Wenzhu Geng Hongxia Zhang Hongxia Zhang Hongxia Zhang Hongxia Zhang Hongxia Zhang Houjun Zhou Houjun Zhou Houjun Zhou Houjun Zhou Houjun Zhou |
| author_sort | Yanwen Wang |
| collection | DOAJ |
| description | IntroductionThe sucrose synthase (SUS), a crucial enzyme in the sucrose metabolism, is encoded by a multigene family in plant kingdom. MethodsIn our study, we utilized bioinformatics tools to identify and characterize the members of the SUS gene family within the blueberry genome. Our analysis encompassed the physicochemical properties, gene structures, conserved motifs, promoter cis-acting elements, chromosomal locations, evolutionary relationships and expression profiles of these family members, allowing us to predict their potential functions. ResultsWe identified seven distinct SUS genes, mapped across six chromosomes, showcasing the complexity of this gene family in blueberries. Phylogenetic analysis, constructed through a multi-species phylogenetic tree, revealed that the SUS gene family can be categorized into three subfamilies: SUS I, SUS II and SUS III. Notable variations were observed among the VdSUS gene family members, particularly in the number of amino acids, molecular weight, isoelectric point, and hydrophobicity of the encoded proteins. Intriguingly, our predictive analysis of the promoter regions of VdSUS genes uncovered a wealth of cis-acting elements linked to light response, hormonal regulation, and stress responses, suggesting a role in adaptive mechanisms. Expression studies indicated that VdSUS genes were highly expressed in fruit tissues, with the application of exogenous sucrose leading to significant downregulation of VdSUS2, VdSUS3 and VdSUS6. Furthermore, the expression of VdSUS genes was found to be responsive to abiotic stresses, such as salt, drought, and low temperatures, with varying degrees of upregulation or downregulation observed. Most notably, the overexpression of VdSUS4 in Arabidopsis thaliana resulted in enhanced tolerance to salt stress. DiscussionThese findings have shed new light on the multifaceted roles of VdSUS gene family members in the complex physiological processes of blueberries, highlighting their potential in the context of stress adaptation and fruit development. |
| format | Article |
| id | doaj-art-d061688431fa41f99c7be0f30e3fe06f |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-06-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-d061688431fa41f99c7be0f30e3fe06f2025-08-20T02:24:04ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-06-011610.3389/fpls.2025.15811821581182The sucrose synthase gene family in blueberry (Vaccinium darrowii): functional insights into the role of VdSUS4 in salt stress toleranceYanwen Wang0Yanwen Wang1Yanwen Wang2Lei Yang3Lei Yang4Lei Yang5Lei Yang6Lei Yang7Wenzhu Geng8Wenzhu Geng9Wenzhu Geng10Hongxia Zhang11Hongxia Zhang12Hongxia Zhang13Hongxia Zhang14Hongxia Zhang15Houjun Zhou16Houjun Zhou17Houjun Zhou18Houjun Zhou19Houjun Zhou20The Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai, Shandong, ChinaCollege of Horticulture, Ludong University, Yantai, Shandong, ChinaYantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Ludong University, Yantai, Shandong, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai, Shandong, ChinaCollege of Horticulture, Ludong University, Yantai, Shandong, ChinaYantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Ludong University, Yantai, Shandong, ChinaResearch and Development Department, Bestplant (Shandong) Stem Cell Engineering Co., Ltd., Yantai, Shandong, ChinaZhaoyuan Shenghui Agricultural Technology Development Co., Ltd, Zhaoyuan, Shandong, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai, Shandong, ChinaCollege of Horticulture, Ludong University, Yantai, Shandong, ChinaYantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Ludong University, Yantai, Shandong, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai, Shandong, ChinaCollege of Horticulture, Ludong University, Yantai, Shandong, ChinaYantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Ludong University, Yantai, Shandong, ChinaResearch and Development Department, Bestplant (Shandong) Stem Cell Engineering Co., Ltd., Yantai, Shandong, ChinaZhaoyuan Shenghui Agricultural Technology Development Co., Ltd, Zhaoyuan, Shandong, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai, Shandong, ChinaCollege of Horticulture, Ludong University, Yantai, Shandong, ChinaYantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Ludong University, Yantai, Shandong, ChinaResearch and Development Department, Bestplant (Shandong) Stem Cell Engineering Co., Ltd., Yantai, Shandong, ChinaZhaoyuan Shenghui Agricultural Technology Development Co., Ltd, Zhaoyuan, Shandong, ChinaIntroductionThe sucrose synthase (SUS), a crucial enzyme in the sucrose metabolism, is encoded by a multigene family in plant kingdom. MethodsIn our study, we utilized bioinformatics tools to identify and characterize the members of the SUS gene family within the blueberry genome. Our analysis encompassed the physicochemical properties, gene structures, conserved motifs, promoter cis-acting elements, chromosomal locations, evolutionary relationships and expression profiles of these family members, allowing us to predict their potential functions. ResultsWe identified seven distinct SUS genes, mapped across six chromosomes, showcasing the complexity of this gene family in blueberries. Phylogenetic analysis, constructed through a multi-species phylogenetic tree, revealed that the SUS gene family can be categorized into three subfamilies: SUS I, SUS II and SUS III. Notable variations were observed among the VdSUS gene family members, particularly in the number of amino acids, molecular weight, isoelectric point, and hydrophobicity of the encoded proteins. Intriguingly, our predictive analysis of the promoter regions of VdSUS genes uncovered a wealth of cis-acting elements linked to light response, hormonal regulation, and stress responses, suggesting a role in adaptive mechanisms. Expression studies indicated that VdSUS genes were highly expressed in fruit tissues, with the application of exogenous sucrose leading to significant downregulation of VdSUS2, VdSUS3 and VdSUS6. Furthermore, the expression of VdSUS genes was found to be responsive to abiotic stresses, such as salt, drought, and low temperatures, with varying degrees of upregulation or downregulation observed. Most notably, the overexpression of VdSUS4 in Arabidopsis thaliana resulted in enhanced tolerance to salt stress. DiscussionThese findings have shed new light on the multifaceted roles of VdSUS gene family members in the complex physiological processes of blueberries, highlighting their potential in the context of stress adaptation and fruit development.https://www.frontiersin.org/articles/10.3389/fpls.2025.1581182/fullblueberrysucrose synthasebioinformaticstissue-specific expressionsalt stress |
| spellingShingle | Yanwen Wang Yanwen Wang Yanwen Wang Lei Yang Lei Yang Lei Yang Lei Yang Lei Yang Wenzhu Geng Wenzhu Geng Wenzhu Geng Hongxia Zhang Hongxia Zhang Hongxia Zhang Hongxia Zhang Hongxia Zhang Houjun Zhou Houjun Zhou Houjun Zhou Houjun Zhou Houjun Zhou The sucrose synthase gene family in blueberry (Vaccinium darrowii): functional insights into the role of VdSUS4 in salt stress tolerance Frontiers in Plant Science blueberry sucrose synthase bioinformatics tissue-specific expression salt stress |
| title | The sucrose synthase gene family in blueberry (Vaccinium darrowii): functional insights into the role of VdSUS4 in salt stress tolerance |
| title_full | The sucrose synthase gene family in blueberry (Vaccinium darrowii): functional insights into the role of VdSUS4 in salt stress tolerance |
| title_fullStr | The sucrose synthase gene family in blueberry (Vaccinium darrowii): functional insights into the role of VdSUS4 in salt stress tolerance |
| title_full_unstemmed | The sucrose synthase gene family in blueberry (Vaccinium darrowii): functional insights into the role of VdSUS4 in salt stress tolerance |
| title_short | The sucrose synthase gene family in blueberry (Vaccinium darrowii): functional insights into the role of VdSUS4 in salt stress tolerance |
| title_sort | sucrose synthase gene family in blueberry vaccinium darrowii functional insights into the role of vdsus4 in salt stress tolerance |
| topic | blueberry sucrose synthase bioinformatics tissue-specific expression salt stress |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1581182/full |
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