Tissue Engineering to Repair Diaphragmatic Defect in a Rat Model
Tissue engineering is an emerging strategy for repairing damaged tissues or organs. The current study explored using decellularized rat diaphragm scaffolds combined with human amniotic fluid-derived multipotent stromal cells (hAFMSC) to provide a scaffold, stem cell construct that would allow struct...
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| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/1764523 |
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| author | G. P. Liao Y. Choi K. Vojnits H. Xue K. Aroom F. Meng H. Y. Pan R. A. Hetz C. J. Corkins T. G. Hughes F. Triolo A. Johnson Kenneth J. Moise K. P. Lally C. S. Cox Y. Li |
| author_facet | G. P. Liao Y. Choi K. Vojnits H. Xue K. Aroom F. Meng H. Y. Pan R. A. Hetz C. J. Corkins T. G. Hughes F. Triolo A. Johnson Kenneth J. Moise K. P. Lally C. S. Cox Y. Li |
| author_sort | G. P. Liao |
| collection | DOAJ |
| description | Tissue engineering is an emerging strategy for repairing damaged tissues or organs. The current study explored using decellularized rat diaphragm scaffolds combined with human amniotic fluid-derived multipotent stromal cells (hAFMSC) to provide a scaffold, stem cell construct that would allow structural barrier function during tissue ingrowth/regeneration. We created an innovative cell infusion system that allowed hAFMSC to embed into scaffolds and then implanted the composite tissues into rats with surgically created left-sided diaphragmatic defects. Control rats received decellularized diaphragm scaffolds alone. We found that the composite tissues that combined hAFMSCs demonstrated improved physiological function as well as the muscular-tendon structure, compared with the native contralateral hemidiaphragm of the same rat. Our results indicate that the decellularized diaphragm scaffolds are a potential support material for diaphragmatic hernia repair and the composite grafts with hAFMSC are able to accelerate the functional recovery of diaphragmatic hernia. |
| format | Article |
| id | doaj-art-d041f6eb7f684b2ca27aee73c8a0c1f0 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-d041f6eb7f684b2ca27aee73c8a0c1f02025-08-20T02:20:05ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/17645231764523Tissue Engineering to Repair Diaphragmatic Defect in a Rat ModelG. P. Liao0Y. Choi1K. Vojnits2H. Xue3K. Aroom4F. Meng5H. Y. Pan6R. A. Hetz7C. J. Corkins8T. G. Hughes9F. Triolo10A. Johnson11Kenneth J. Moise12K. P. Lally13C. S. Cox14Y. Li15Department of Pediatric Surgery, University of Texas, McGovern Medical School at Houston, Houston, TX, USADepartment of Pediatric Surgery, University of Texas, McGovern Medical School at Houston, Houston, TX, USADepartment of Pediatric Surgery, University of Texas, McGovern Medical School at Houston, Houston, TX, USADepartment of Pediatric Surgery, University of Texas, McGovern Medical School at Houston, Houston, TX, USADepartment of Pediatric Surgery, University of Texas, McGovern Medical School at Houston, Houston, TX, USADepartment of Pediatric Surgery, University of Texas, McGovern Medical School at Houston, Houston, TX, USADepartment of Pediatric Surgery, University of Texas, McGovern Medical School at Houston, Houston, TX, USADepartment of Pediatric Surgery, University of Texas, McGovern Medical School at Houston, Houston, TX, USADepartment of Pediatric Surgery, University of Texas, McGovern Medical School at Houston, Houston, TX, USADepartment of Pediatric Surgery, University of Texas, McGovern Medical School at Houston, Houston, TX, USADepartment of Pediatric Surgery, University of Texas, McGovern Medical School at Houston, Houston, TX, USADepartment of Obstetrics, Gynecology, and Reproductive Sciences, University of Texas, McGovern Medical School at Houston, Houston, TX 77030, USADepartment of Obstetrics, Gynecology, and Reproductive Sciences, University of Texas, McGovern Medical School at Houston, Houston, TX 77030, USADepartment of Pediatric Surgery, University of Texas, McGovern Medical School at Houston, Houston, TX, USADepartment of Pediatric Surgery, University of Texas, McGovern Medical School at Houston, Houston, TX, USADepartment of Pediatric Surgery, University of Texas, McGovern Medical School at Houston, Houston, TX, USATissue engineering is an emerging strategy for repairing damaged tissues or organs. The current study explored using decellularized rat diaphragm scaffolds combined with human amniotic fluid-derived multipotent stromal cells (hAFMSC) to provide a scaffold, stem cell construct that would allow structural barrier function during tissue ingrowth/regeneration. We created an innovative cell infusion system that allowed hAFMSC to embed into scaffolds and then implanted the composite tissues into rats with surgically created left-sided diaphragmatic defects. Control rats received decellularized diaphragm scaffolds alone. We found that the composite tissues that combined hAFMSCs demonstrated improved physiological function as well as the muscular-tendon structure, compared with the native contralateral hemidiaphragm of the same rat. Our results indicate that the decellularized diaphragm scaffolds are a potential support material for diaphragmatic hernia repair and the composite grafts with hAFMSC are able to accelerate the functional recovery of diaphragmatic hernia.http://dx.doi.org/10.1155/2017/1764523 |
| spellingShingle | G. P. Liao Y. Choi K. Vojnits H. Xue K. Aroom F. Meng H. Y. Pan R. A. Hetz C. J. Corkins T. G. Hughes F. Triolo A. Johnson Kenneth J. Moise K. P. Lally C. S. Cox Y. Li Tissue Engineering to Repair Diaphragmatic Defect in a Rat Model Stem Cells International |
| title | Tissue Engineering to Repair Diaphragmatic Defect in a Rat Model |
| title_full | Tissue Engineering to Repair Diaphragmatic Defect in a Rat Model |
| title_fullStr | Tissue Engineering to Repair Diaphragmatic Defect in a Rat Model |
| title_full_unstemmed | Tissue Engineering to Repair Diaphragmatic Defect in a Rat Model |
| title_short | Tissue Engineering to Repair Diaphragmatic Defect in a Rat Model |
| title_sort | tissue engineering to repair diaphragmatic defect in a rat model |
| url | http://dx.doi.org/10.1155/2017/1764523 |
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