A universal DNA microarray for rapid fish species authentication
DNA microarrays are now used in fields such as gene expression analysis, pathogen/virus detection and identification of biomarkers. Although they have been used in the food sector for species identification, they detect a limited number of species and are thus less suited for fishery products due to...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Food Chemistry: Molecular Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666566225000024 |
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| author | Patrizia Bade Sebastian Stix Kristina Kappel Jan Fritsche Ilka Haase Andrew Torda Nils Wax Markus Fischer Dirk Brandis Ute Schröder |
| author_facet | Patrizia Bade Sebastian Stix Kristina Kappel Jan Fritsche Ilka Haase Andrew Torda Nils Wax Markus Fischer Dirk Brandis Ute Schröder |
| author_sort | Patrizia Bade |
| collection | DOAJ |
| description | DNA microarrays are now used in fields such as gene expression analysis, pathogen/virus detection and identification of biomarkers. Although they have been used in the food sector for species identification, they detect a limited number of species and are thus less suited for fishery products due to the large variety of traded species. Here, the aim of this proof-of-principle study was to design a universal DNA microarray that should be able to distinguish all fish species by comparing hybridization signal patterns from samples with patterns obtained from reference specimens. A universal set of 100 DNA probes (based on the genetic marker genes 16S ribosomal RNA and cytochrome b) generated species-specific DNA probe patterns for all 86 analyzed fish specimens. This new screening method shows potential to authenticate specimens from all fish species and by this could play an important role in fighting fraudulent practices and adulteration in the seafood sector. |
| format | Article |
| id | doaj-art-d03f69dd33964a23ba57d6f4265a6898 |
| institution | Kabale University |
| issn | 2666-5662 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: Molecular Sciences |
| spelling | doaj-art-d03f69dd33964a23ba57d6f4265a68982025-01-24T04:45:47ZengElsevierFood Chemistry: Molecular Sciences2666-56622025-06-0110100241A universal DNA microarray for rapid fish species authenticationPatrizia Bade0Sebastian Stix1Kristina Kappel2Jan Fritsche3Ilka Haase4Andrew Torda5Nils Wax6Markus Fischer7Dirk Brandis8Ute Schröder9National Reference Centre for Authentic Food, Max Rubner-Institut (MRI), Hermann-Weigmann-Str. 1, 24103 Kiel, GermanyDepartment of Safety and Quality of Milk and Fish Products, Max Rubner-Institut (MRI), Hermann-Weigmann-Str. 1, 24103 Kiel, GermanyNational Reference Centre for Authentic Food, Max Rubner-Institut (MRI), Hermann-Weigmann-Str. 1, 24103 Kiel, Germany; Corresponding author.Department of Safety and Quality of Milk and Fish Products, Max Rubner-Institut (MRI), Hermann-Weigmann-Str. 1, 24103 Kiel, GermanyNational Reference Centre for Authentic Food, Max Rubner-Institut (MRI), E.-C.-Baumann-Str. 20, 95326 Kulmbach, GermanyCentre for Bioinformatics, University of Hamburg, Albert-Einstein-Ring 8, 22761 Hamburg, GermanyHamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, GermanyHamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, GermanyZoological Museum of the University of Kiel, Hegewischstraße 3, 24105 Kiel, GermanyDepartment of Safety and Quality of Milk and Fish Products, Max Rubner-Institut (MRI), Hermann-Weigmann-Str. 1, 24103 Kiel, GermanyDNA microarrays are now used in fields such as gene expression analysis, pathogen/virus detection and identification of biomarkers. Although they have been used in the food sector for species identification, they detect a limited number of species and are thus less suited for fishery products due to the large variety of traded species. Here, the aim of this proof-of-principle study was to design a universal DNA microarray that should be able to distinguish all fish species by comparing hybridization signal patterns from samples with patterns obtained from reference specimens. A universal set of 100 DNA probes (based on the genetic marker genes 16S ribosomal RNA and cytochrome b) generated species-specific DNA probe patterns for all 86 analyzed fish specimens. This new screening method shows potential to authenticate specimens from all fish species and by this could play an important role in fighting fraudulent practices and adulteration in the seafood sector.http://www.sciencedirect.com/science/article/pii/S2666566225000024DNA chipFood fraudUniversal DNA probesDNA hybridizationScreening methodIn silico |
| spellingShingle | Patrizia Bade Sebastian Stix Kristina Kappel Jan Fritsche Ilka Haase Andrew Torda Nils Wax Markus Fischer Dirk Brandis Ute Schröder A universal DNA microarray for rapid fish species authentication Food Chemistry: Molecular Sciences DNA chip Food fraud Universal DNA probes DNA hybridization Screening method In silico |
| title | A universal DNA microarray for rapid fish species authentication |
| title_full | A universal DNA microarray for rapid fish species authentication |
| title_fullStr | A universal DNA microarray for rapid fish species authentication |
| title_full_unstemmed | A universal DNA microarray for rapid fish species authentication |
| title_short | A universal DNA microarray for rapid fish species authentication |
| title_sort | universal dna microarray for rapid fish species authentication |
| topic | DNA chip Food fraud Universal DNA probes DNA hybridization Screening method In silico |
| url | http://www.sciencedirect.com/science/article/pii/S2666566225000024 |
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