Supramolecular prodrug inspiried by the Rhizoma Coptidis-Fructus Mume herbal pair alleviated inflammatory diseases by inhibiting pyroptosis
Sustained inflammatory responses are closely related to various severe diseases, and inhibiting the excessive activation of inflammasomes and pyroptosis has significant implications for clinical treatment. Natural products have garnered considerable concern for the treatment of inflammation. Huangli...
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Elsevier
2025-02-01
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| Series: | Journal of Pharmaceutical Analysis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177924001539 |
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| author | Wenhui Qian Bei Zhang Ming Gao Yuting Wang Jiachen Shen Dongbing Liang Chao Wang Wei Wei Xing Pan Qiuying Yan Dongdong Sun Dong Zhu Haibo Cheng |
| author_facet | Wenhui Qian Bei Zhang Ming Gao Yuting Wang Jiachen Shen Dongbing Liang Chao Wang Wei Wei Xing Pan Qiuying Yan Dongdong Sun Dong Zhu Haibo Cheng |
| author_sort | Wenhui Qian |
| collection | DOAJ |
| description | Sustained inflammatory responses are closely related to various severe diseases, and inhibiting the excessive activation of inflammasomes and pyroptosis has significant implications for clinical treatment. Natural products have garnered considerable concern for the treatment of inflammation. Huanglian-Wumei decoction (HLWMD) is a classic prescription used for treating inflammatory diseases, but the necessity of their combination and the exact underlying anti-inflammatory mechanism have not yet been elucidated. Inspired by the supramolecular self-assembly strategy and natural drug compatibility theory, we successfully obtained berberine (BBR)-chlorogenic acid (CGA) supramolecular (BCS), which is an herbal pair from HLWMD. Using a series of characterization methods, we confirmed the self-assembly mechanism of BCS. BBR and CGA were self-assembled and stacked into amphiphilic spherical supramolecules in a 2:1 molar ratio, driven by electrostatic interactions, hydrophobic interactions, and π–π stacking; the hydrophilic fragments of CGA were outside, and the hydrophobic fragments of BBR were inside. This stacking pattern significantly improved the anti-inflammatory performance of BCS compared with that of single free molecules. Compared with free molecules, BCS significantly attenuated the release of multiple inflammatory mediators and lipopolysaccharide (LPS)-induced pyroptosis. Its anti-inflammatory mechanism is closely related to the inhibition of intracellular nuclear factor-kappaB (NF-κB) p65 phosphorylation and the noncanonical pyroptosis signalling pathway mediated by caspase-11. |
| format | Article |
| id | doaj-art-d035e165c28a405dba481076bbe63124 |
| institution | Kabale University |
| issn | 2095-1779 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj-art-d035e165c28a405dba481076bbe631242025-02-02T05:27:05ZengElsevierJournal of Pharmaceutical Analysis2095-17792025-02-01152101056Supramolecular prodrug inspiried by the Rhizoma Coptidis-Fructus Mume herbal pair alleviated inflammatory diseases by inhibiting pyroptosisWenhui Qian0Bei Zhang1Ming Gao2Yuting Wang3Jiachen Shen4Dongbing Liang5Chao Wang6Wei Wei7Xing Pan8Qiuying Yan9Dongdong Sun10Dong Zhu11Haibo Cheng12School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaDepartment of Pharmacy, Jinling Hospital, Nanjing University School of Medicine, Nanjing, 210002, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaJiangsu Collaborative Innovation Center of Traditional Chinese Medicine Prevention and Treatment of Tumor, Nanjing, 210023, ChinaJiangsu Collaborative Innovation Center of Traditional Chinese Medicine Prevention and Treatment of Tumor, Nanjing, 210023, China; Corresponding author.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China; Corresponding author.Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine Prevention and Treatment of Tumor, Nanjing, 210023, China; Corresponding author.Sustained inflammatory responses are closely related to various severe diseases, and inhibiting the excessive activation of inflammasomes and pyroptosis has significant implications for clinical treatment. Natural products have garnered considerable concern for the treatment of inflammation. Huanglian-Wumei decoction (HLWMD) is a classic prescription used for treating inflammatory diseases, but the necessity of their combination and the exact underlying anti-inflammatory mechanism have not yet been elucidated. Inspired by the supramolecular self-assembly strategy and natural drug compatibility theory, we successfully obtained berberine (BBR)-chlorogenic acid (CGA) supramolecular (BCS), which is an herbal pair from HLWMD. Using a series of characterization methods, we confirmed the self-assembly mechanism of BCS. BBR and CGA were self-assembled and stacked into amphiphilic spherical supramolecules in a 2:1 molar ratio, driven by electrostatic interactions, hydrophobic interactions, and π–π stacking; the hydrophilic fragments of CGA were outside, and the hydrophobic fragments of BBR were inside. This stacking pattern significantly improved the anti-inflammatory performance of BCS compared with that of single free molecules. Compared with free molecules, BCS significantly attenuated the release of multiple inflammatory mediators and lipopolysaccharide (LPS)-induced pyroptosis. Its anti-inflammatory mechanism is closely related to the inhibition of intracellular nuclear factor-kappaB (NF-κB) p65 phosphorylation and the noncanonical pyroptosis signalling pathway mediated by caspase-11.http://www.sciencedirect.com/science/article/pii/S2095177924001539Self-assemblyAnti-inflammationSupramolecularPyroptosisTraditional Chinese medicine |
| spellingShingle | Wenhui Qian Bei Zhang Ming Gao Yuting Wang Jiachen Shen Dongbing Liang Chao Wang Wei Wei Xing Pan Qiuying Yan Dongdong Sun Dong Zhu Haibo Cheng Supramolecular prodrug inspiried by the Rhizoma Coptidis-Fructus Mume herbal pair alleviated inflammatory diseases by inhibiting pyroptosis Journal of Pharmaceutical Analysis Self-assembly Anti-inflammation Supramolecular Pyroptosis Traditional Chinese medicine |
| title | Supramolecular prodrug inspiried by the Rhizoma Coptidis-Fructus Mume herbal pair alleviated inflammatory diseases by inhibiting pyroptosis |
| title_full | Supramolecular prodrug inspiried by the Rhizoma Coptidis-Fructus Mume herbal pair alleviated inflammatory diseases by inhibiting pyroptosis |
| title_fullStr | Supramolecular prodrug inspiried by the Rhizoma Coptidis-Fructus Mume herbal pair alleviated inflammatory diseases by inhibiting pyroptosis |
| title_full_unstemmed | Supramolecular prodrug inspiried by the Rhizoma Coptidis-Fructus Mume herbal pair alleviated inflammatory diseases by inhibiting pyroptosis |
| title_short | Supramolecular prodrug inspiried by the Rhizoma Coptidis-Fructus Mume herbal pair alleviated inflammatory diseases by inhibiting pyroptosis |
| title_sort | supramolecular prodrug inspiried by the rhizoma coptidis fructus mume herbal pair alleviated inflammatory diseases by inhibiting pyroptosis |
| topic | Self-assembly Anti-inflammation Supramolecular Pyroptosis Traditional Chinese medicine |
| url | http://www.sciencedirect.com/science/article/pii/S2095177924001539 |
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