Mesenchymal Stromal Cells from Osteoarthritic Synovium Are a Distinct Population Compared to Their Bone-Marrow Counterparts regarding Surface Marker Distribution and Immunomodulation of Allogeneic CD4+ T-Cell Cultures
Introduction. The participation of an inflammatory joint milieu has been described in osteoarthritis (OA) pathogenesis. Mesenchymal stromal cells (MSCs) play an important role in modulating inflammatory processes. Based on previous studies in an allogeneic T-cell coculture model, we aimed at further...
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| Format: | Article |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/6579463 |
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| author | Sebastien Hagmann Claudia Rimmele Florin Bucur Thomas Dreher Felix Zeifang Babak Moradi Tobias Gotterbarm |
| author_facet | Sebastien Hagmann Claudia Rimmele Florin Bucur Thomas Dreher Felix Zeifang Babak Moradi Tobias Gotterbarm |
| author_sort | Sebastien Hagmann |
| collection | DOAJ |
| description | Introduction. The participation of an inflammatory joint milieu has been described in osteoarthritis (OA) pathogenesis. Mesenchymal stromal cells (MSCs) play an important role in modulating inflammatory processes. Based on previous studies in an allogeneic T-cell coculture model, we aimed at further determining the role of synovial MSCs in OA pathogenesis. Methods. Bone-marrow (BM) and synovial membrane (SM) MSCs from hip joints of late stage OA patients and CD4+ T-cells from healthy donors were analysed regarding surface marker expression before and after coculture. Proliferation upon CD3/CD28 stimulation and cytokine analyses were compared between MSCs. Results. SM-MSCs differed from BM-MSCs in several surface markers and their osteogenic differentiation potential. Cocultures of both MSCs with CD4+ T-cells resulted in recruitment of CD45RA+ FoxP3+ regulatory T-cells. Upon stimulation, only SM-MSCs suppressed CD4+ T-cell proliferation, while both SM-MSCs and BM-MSCs modified cytokine profiles through suppressing IL-2 and TNF-α as well as increasing IL-6 secretion. Conclusions. Synovial MSCs from OA joints are a unique fraction that can be distinguished from their bone-marrow derived counterparts. Their unique ability to suppress CD3/CD28 induced CD4+ T-cell proliferation makes them a potential target for future therapeutic approaches. |
| format | Article |
| id | doaj-art-d0283096ba2d4c2181f8bc1f348e3e9b |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
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| spelling | doaj-art-d0283096ba2d4c2181f8bc1f348e3e9b2025-02-03T06:08:03ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/65794636579463Mesenchymal Stromal Cells from Osteoarthritic Synovium Are a Distinct Population Compared to Their Bone-Marrow Counterparts regarding Surface Marker Distribution and Immunomodulation of Allogeneic CD4+ T-Cell CulturesSebastien Hagmann0Claudia Rimmele1Florin Bucur2Thomas Dreher3Felix Zeifang4Babak Moradi5Tobias Gotterbarm6Clinic for Orthopedics and Trauma Surgery, Center for Orthopedics, Trauma Surgery and Spinal Cords Injury, Heidelberg University Hospital, Schlierbacher Landstrasse 200a, 69118 Heidelberg, GermanyClinic for Orthopedics and Trauma Surgery, Center for Orthopedics, Trauma Surgery and Spinal Cords Injury, Heidelberg University Hospital, Schlierbacher Landstrasse 200a, 69118 Heidelberg, GermanyClinic for Orthopedics and Trauma Surgery, Center for Orthopedics, Trauma Surgery and Spinal Cords Injury, Heidelberg University Hospital, Schlierbacher Landstrasse 200a, 69118 Heidelberg, GermanyClinic for Orthopedics and Trauma Surgery, Center for Orthopedics, Trauma Surgery and Spinal Cords Injury, Heidelberg University Hospital, Schlierbacher Landstrasse 200a, 69118 Heidelberg, GermanyClinic for Orthopedics and Trauma Surgery, Center for Orthopedics, Trauma Surgery and Spinal Cords Injury, Heidelberg University Hospital, Schlierbacher Landstrasse 200a, 69118 Heidelberg, GermanyClinic for Orthopedics and Trauma Surgery, Center for Orthopedics, Trauma Surgery and Spinal Cords Injury, Heidelberg University Hospital, Schlierbacher Landstrasse 200a, 69118 Heidelberg, GermanyClinic for Orthopedics and Trauma Surgery, Center for Orthopedics, Trauma Surgery and Spinal Cords Injury, Heidelberg University Hospital, Schlierbacher Landstrasse 200a, 69118 Heidelberg, GermanyIntroduction. The participation of an inflammatory joint milieu has been described in osteoarthritis (OA) pathogenesis. Mesenchymal stromal cells (MSCs) play an important role in modulating inflammatory processes. Based on previous studies in an allogeneic T-cell coculture model, we aimed at further determining the role of synovial MSCs in OA pathogenesis. Methods. Bone-marrow (BM) and synovial membrane (SM) MSCs from hip joints of late stage OA patients and CD4+ T-cells from healthy donors were analysed regarding surface marker expression before and after coculture. Proliferation upon CD3/CD28 stimulation and cytokine analyses were compared between MSCs. Results. SM-MSCs differed from BM-MSCs in several surface markers and their osteogenic differentiation potential. Cocultures of both MSCs with CD4+ T-cells resulted in recruitment of CD45RA+ FoxP3+ regulatory T-cells. Upon stimulation, only SM-MSCs suppressed CD4+ T-cell proliferation, while both SM-MSCs and BM-MSCs modified cytokine profiles through suppressing IL-2 and TNF-α as well as increasing IL-6 secretion. Conclusions. Synovial MSCs from OA joints are a unique fraction that can be distinguished from their bone-marrow derived counterparts. Their unique ability to suppress CD3/CD28 induced CD4+ T-cell proliferation makes them a potential target for future therapeutic approaches.http://dx.doi.org/10.1155/2016/6579463 |
| spellingShingle | Sebastien Hagmann Claudia Rimmele Florin Bucur Thomas Dreher Felix Zeifang Babak Moradi Tobias Gotterbarm Mesenchymal Stromal Cells from Osteoarthritic Synovium Are a Distinct Population Compared to Their Bone-Marrow Counterparts regarding Surface Marker Distribution and Immunomodulation of Allogeneic CD4+ T-Cell Cultures Stem Cells International |
| title | Mesenchymal Stromal Cells from Osteoarthritic Synovium Are a Distinct Population Compared to Their Bone-Marrow Counterparts regarding Surface Marker Distribution and Immunomodulation of Allogeneic CD4+ T-Cell Cultures |
| title_full | Mesenchymal Stromal Cells from Osteoarthritic Synovium Are a Distinct Population Compared to Their Bone-Marrow Counterparts regarding Surface Marker Distribution and Immunomodulation of Allogeneic CD4+ T-Cell Cultures |
| title_fullStr | Mesenchymal Stromal Cells from Osteoarthritic Synovium Are a Distinct Population Compared to Their Bone-Marrow Counterparts regarding Surface Marker Distribution and Immunomodulation of Allogeneic CD4+ T-Cell Cultures |
| title_full_unstemmed | Mesenchymal Stromal Cells from Osteoarthritic Synovium Are a Distinct Population Compared to Their Bone-Marrow Counterparts regarding Surface Marker Distribution and Immunomodulation of Allogeneic CD4+ T-Cell Cultures |
| title_short | Mesenchymal Stromal Cells from Osteoarthritic Synovium Are a Distinct Population Compared to Their Bone-Marrow Counterparts regarding Surface Marker Distribution and Immunomodulation of Allogeneic CD4+ T-Cell Cultures |
| title_sort | mesenchymal stromal cells from osteoarthritic synovium are a distinct population compared to their bone marrow counterparts regarding surface marker distribution and immunomodulation of allogeneic cd4 t cell cultures |
| url | http://dx.doi.org/10.1155/2016/6579463 |
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