A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells
We reported here a turn-on fluorescent probe (1) for the detection of cysteine (Cys) by incorporating the recognition unit of 2,4-dinitrobenzenesulfonyl ester (DNBS) to a coumarin derivative. The structure of the obtained probe was confirmed by NMR and HRMS techniques. The probe shows a remarkable f...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/1986468 |
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| author | Xiaohua Ma Guoguang Wu Yuehua Zhao Zibo Yuan Yu Zhang Ning Xia Mengnan Yang Lin Liu |
| author_facet | Xiaohua Ma Guoguang Wu Yuehua Zhao Zibo Yuan Yu Zhang Ning Xia Mengnan Yang Lin Liu |
| author_sort | Xiaohua Ma |
| collection | DOAJ |
| description | We reported here a turn-on fluorescent probe (1) for the detection of cysteine (Cys) by incorporating the recognition unit of 2,4-dinitrobenzenesulfonyl ester (DNBS) to a coumarin derivative. The structure of the obtained probe was confirmed by NMR and HRMS techniques. The probe shows a remarkable fluorescence off-on response (∼52-fold) by the reaction with Cys in 100% aqueous buffer. The sensing mechanism was verified by the HPLC test. Probe 1 also displays high selectivity towards Cys. The detection limit was calculated to be 23 nM. Moreover, cellular experiments demonstrated that the probe is highly biocompatible and can be used for monitoring intracellular Cys. |
| format | Article |
| id | doaj-art-d027b99763a34dafbfc9a931d3bab1ec |
| institution | OA Journals |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-d027b99763a34dafbfc9a931d3bab1ec2025-08-20T02:20:03ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/19864681986468A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living CellsXiaohua Ma0Guoguang Wu1Yuehua Zhao2Zibo Yuan3Yu Zhang4Ning Xia5Mengnan Yang6Lin Liu7School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou, Jiangsu 221116, ChinaSchool of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou, Jiangsu 221116, ChinaKey Laboratory of New Optoelectronic Functional Materials (Henan Province), College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, ChinaKey Laboratory of New Optoelectronic Functional Materials (Henan Province), College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, ChinaKey Laboratory of New Optoelectronic Functional Materials (Henan Province), College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, ChinaKey Laboratory of New Optoelectronic Functional Materials (Henan Province), College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, ChinaKey Laboratory of New Optoelectronic Functional Materials (Henan Province), College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, ChinaKey Laboratory of New Optoelectronic Functional Materials (Henan Province), College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, ChinaWe reported here a turn-on fluorescent probe (1) for the detection of cysteine (Cys) by incorporating the recognition unit of 2,4-dinitrobenzenesulfonyl ester (DNBS) to a coumarin derivative. The structure of the obtained probe was confirmed by NMR and HRMS techniques. The probe shows a remarkable fluorescence off-on response (∼52-fold) by the reaction with Cys in 100% aqueous buffer. The sensing mechanism was verified by the HPLC test. Probe 1 also displays high selectivity towards Cys. The detection limit was calculated to be 23 nM. Moreover, cellular experiments demonstrated that the probe is highly biocompatible and can be used for monitoring intracellular Cys.http://dx.doi.org/10.1155/2018/1986468 |
| spellingShingle | Xiaohua Ma Guoguang Wu Yuehua Zhao Zibo Yuan Yu Zhang Ning Xia Mengnan Yang Lin Liu A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells Journal of Analytical Methods in Chemistry |
| title | A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells |
| title_full | A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells |
| title_fullStr | A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells |
| title_full_unstemmed | A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells |
| title_short | A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells |
| title_sort | turn on fluorescent probe for sensitive detection of cysteine in a fully aqueous environment and in living cells |
| url | http://dx.doi.org/10.1155/2018/1986468 |
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