Functional design of sage seed galactomannan based-films with gelled double emulsion strategy activated by co-incorporating rosemary extract and Zataria multiflora essential oil

This study explored active film development using gelatin and sage seed gum through various gelled double emulsions strategies, including W1g/O/W2, W1/Og/W2, and W1g/Og/W2 which were fortified with rosemary extract (RE) and Zataria multiflora essential oil (ZMEO). SEM images confirmed the successful...

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Main Authors: Seyyed Mojtaba Hosseini, Marzieh Moosavi-Nasab, Marjan Majdinasab, Hadi Hashemi, Fatemeh Ghiasi
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Carbohydrate Polymer Technologies and Applications
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666893925001380
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author Seyyed Mojtaba Hosseini
Marzieh Moosavi-Nasab
Marjan Majdinasab
Hadi Hashemi
Fatemeh Ghiasi
author_facet Seyyed Mojtaba Hosseini
Marzieh Moosavi-Nasab
Marjan Majdinasab
Hadi Hashemi
Fatemeh Ghiasi
author_sort Seyyed Mojtaba Hosseini
collection DOAJ
description This study explored active film development using gelatin and sage seed gum through various gelled double emulsions strategies, including W1g/O/W2, W1/Og/W2, and W1g/Og/W2 which were fortified with rosemary extract (RE) and Zataria multiflora essential oil (ZMEO). SEM images confirmed the successful creation of a denser and smoother film surface by incorporating RE and ZMEO. Although, roughness increased after the gelation of each inner water and oil phase, no pores and cracks were observed in these films. In contrast, microstructural attributes (i.e., smooth and homogeneous surface) were improved in W1g/Og/W2, suggesting the enhancement effect of gelation of both water and oil phases. XRD spectra showed no change in film crystallinity in the presence of RE and ZMEO, however; it was increased after gelation. The incorporation of ZMEO and rosemary extract, as well as the gelation of both inner water and oil phases, modified the mechanical properties in terms of tensile strength and elongation at break. Water vapor permeability, solubility, and swelling of films decreased with the addition of ZMEO due to its hydrophobic nature. Antioxidant activity using the DPPH radical scavenging assay and antibacterial activity against Listeria monocytogenes and Pseudomonas aeruginosa using the determination of inhibition zone also confirmed the significant effect of ZMEO and RE to design active films. The gelation of the inner water and oil phases, individually or simultaneously, had no significant effect on antioxidant activity; however, it enhanced antibacterial activities due to the prolonged exposure of bacteria to the controlled release of bioactives. Therefore, the gelled double emulsion strategy by co-encapsulation of hydrophilic and hydrophobic bioactives can provide functional active films with ideal physical and mechanical properties.
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spelling doaj-art-d025af0a7e07442ba0410e84a89ff0a02025-08-20T03:20:58ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-06-011010080010.1016/j.carpta.2025.100800Functional design of sage seed galactomannan based-films with gelled double emulsion strategy activated by co-incorporating rosemary extract and Zataria multiflora essential oilSeyyed Mojtaba Hosseini0Marzieh Moosavi-Nasab1Marjan Majdinasab2Hadi Hashemi3Fatemeh Ghiasi4Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, IranDepartment of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran; Seafood Processing Research Center, School of Agriculture, Shiraz University, Shiraz, Iran; Corresponding author.Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, IranDepartment of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, IranDepartment of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, IranThis study explored active film development using gelatin and sage seed gum through various gelled double emulsions strategies, including W1g/O/W2, W1/Og/W2, and W1g/Og/W2 which were fortified with rosemary extract (RE) and Zataria multiflora essential oil (ZMEO). SEM images confirmed the successful creation of a denser and smoother film surface by incorporating RE and ZMEO. Although, roughness increased after the gelation of each inner water and oil phase, no pores and cracks were observed in these films. In contrast, microstructural attributes (i.e., smooth and homogeneous surface) were improved in W1g/Og/W2, suggesting the enhancement effect of gelation of both water and oil phases. XRD spectra showed no change in film crystallinity in the presence of RE and ZMEO, however; it was increased after gelation. The incorporation of ZMEO and rosemary extract, as well as the gelation of both inner water and oil phases, modified the mechanical properties in terms of tensile strength and elongation at break. Water vapor permeability, solubility, and swelling of films decreased with the addition of ZMEO due to its hydrophobic nature. Antioxidant activity using the DPPH radical scavenging assay and antibacterial activity against Listeria monocytogenes and Pseudomonas aeruginosa using the determination of inhibition zone also confirmed the significant effect of ZMEO and RE to design active films. The gelation of the inner water and oil phases, individually or simultaneously, had no significant effect on antioxidant activity; however, it enhanced antibacterial activities due to the prolonged exposure of bacteria to the controlled release of bioactives. Therefore, the gelled double emulsion strategy by co-encapsulation of hydrophilic and hydrophobic bioactives can provide functional active films with ideal physical and mechanical properties.http://www.sciencedirect.com/science/article/pii/S2666893925001380Active filmGelled double emulsionEssential oilGelatinSage seed gum
spellingShingle Seyyed Mojtaba Hosseini
Marzieh Moosavi-Nasab
Marjan Majdinasab
Hadi Hashemi
Fatemeh Ghiasi
Functional design of sage seed galactomannan based-films with gelled double emulsion strategy activated by co-incorporating rosemary extract and Zataria multiflora essential oil
Carbohydrate Polymer Technologies and Applications
Active film
Gelled double emulsion
Essential oil
Gelatin
Sage seed gum
title Functional design of sage seed galactomannan based-films with gelled double emulsion strategy activated by co-incorporating rosemary extract and Zataria multiflora essential oil
title_full Functional design of sage seed galactomannan based-films with gelled double emulsion strategy activated by co-incorporating rosemary extract and Zataria multiflora essential oil
title_fullStr Functional design of sage seed galactomannan based-films with gelled double emulsion strategy activated by co-incorporating rosemary extract and Zataria multiflora essential oil
title_full_unstemmed Functional design of sage seed galactomannan based-films with gelled double emulsion strategy activated by co-incorporating rosemary extract and Zataria multiflora essential oil
title_short Functional design of sage seed galactomannan based-films with gelled double emulsion strategy activated by co-incorporating rosemary extract and Zataria multiflora essential oil
title_sort functional design of sage seed galactomannan based films with gelled double emulsion strategy activated by co incorporating rosemary extract and zataria multiflora essential oil
topic Active film
Gelled double emulsion
Essential oil
Gelatin
Sage seed gum
url http://www.sciencedirect.com/science/article/pii/S2666893925001380
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