Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial Cancer

Peroxisome proliferator-activated receptors (PPARs) belong to a family of nuclear hormone receptors acting as transcriptional factors, recently involved also in carcinogenesis. Present study was undertaken to evaluate the presence and subcellular localization of different PPAR isoforms (α, β, γ) in...

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Main Authors: Paweł Knapp, Adrian Chabowski, Agnieszka Błachnio-Zabielska, Katarzyna Jarząbek, Sławomir Wołczyński
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:PPAR Research
Online Access:http://dx.doi.org/10.1155/2012/471524
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author Paweł Knapp
Adrian Chabowski
Agnieszka Błachnio-Zabielska
Katarzyna Jarząbek
Sławomir Wołczyński
author_facet Paweł Knapp
Adrian Chabowski
Agnieszka Błachnio-Zabielska
Katarzyna Jarząbek
Sławomir Wołczyński
author_sort Paweł Knapp
collection DOAJ
description Peroxisome proliferator-activated receptors (PPARs) belong to a family of nuclear hormone receptors acting as transcriptional factors, recently involved also in carcinogenesis. Present study was undertaken to evaluate the presence and subcellular localization of different PPAR isoforms (α, β, γ) in healthy endometrial tissue (n=10) and endometrial carcinoma (FIGO I, endometrioides type, G1, n=35). We sought to analyze PPARs mRNA content as well as protein immunohistochemical expression that was further quantified by Western Blot technique. For both PPARα and PPARβ, protein expression was significantly higher in endometrial cancers compared to normal endometrial mucosa. In opposite, PPARγ protein expression was lower in endometrial cancer cells. In each case, immunohistochemical reaction was confined to the perinuclear and/or nuclear region. At the transcriptional level, the content of mRNA of all PPAR subunits did not follow the protein pattern of changes. These results provide evidence for altered PPAR's protein expression and disregulation of posttranslational processes in endometrial cancers.
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institution Kabale University
issn 1687-4757
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language English
publishDate 2012-01-01
publisher Wiley
record_format Article
series PPAR Research
spelling doaj-art-d019c79a53ff4bdfba890f604624556a2025-02-03T01:23:39ZengWileyPPAR Research1687-47571687-47652012-01-01201210.1155/2012/471524471524Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial CancerPaweł Knapp0Adrian Chabowski1Agnieszka Błachnio-Zabielska2Katarzyna Jarząbek3Sławomir Wołczyński4Department of Gynecology, Medical University of Bialystok, 15-222 Bialystok, PolandDepartment of Physiology, Medical University of Bialystok, 15-222 Bialystok, PolandDepartment of Physiology, Medical University of Bialystok, 15-222 Bialystok, PolandDepartment of Gynecological Endocrinology, Medical University of Bialystok, 15-276 Bialystok, PolandDepartment of Gynecological Endocrinology, Medical University of Bialystok, 15-276 Bialystok, PolandPeroxisome proliferator-activated receptors (PPARs) belong to a family of nuclear hormone receptors acting as transcriptional factors, recently involved also in carcinogenesis. Present study was undertaken to evaluate the presence and subcellular localization of different PPAR isoforms (α, β, γ) in healthy endometrial tissue (n=10) and endometrial carcinoma (FIGO I, endometrioides type, G1, n=35). We sought to analyze PPARs mRNA content as well as protein immunohistochemical expression that was further quantified by Western Blot technique. For both PPARα and PPARβ, protein expression was significantly higher in endometrial cancers compared to normal endometrial mucosa. In opposite, PPARγ protein expression was lower in endometrial cancer cells. In each case, immunohistochemical reaction was confined to the perinuclear and/or nuclear region. At the transcriptional level, the content of mRNA of all PPAR subunits did not follow the protein pattern of changes. These results provide evidence for altered PPAR's protein expression and disregulation of posttranslational processes in endometrial cancers.http://dx.doi.org/10.1155/2012/471524
spellingShingle Paweł Knapp
Adrian Chabowski
Agnieszka Błachnio-Zabielska
Katarzyna Jarząbek
Sławomir Wołczyński
Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial Cancer
PPAR Research
title Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial Cancer
title_full Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial Cancer
title_fullStr Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial Cancer
title_full_unstemmed Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial Cancer
title_short Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial Cancer
title_sort altered peroxisome proliferator activated receptors expression in human endometrial cancer
url http://dx.doi.org/10.1155/2012/471524
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AT katarzynajarzabek alteredperoxisomeproliferatoractivatedreceptorsexpressioninhumanendometrialcancer
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