Potential effect of platelet rich fibrin prepared under different centrifugation protocols on stem cells from the apical papilla
Objectives: The present work was designed to evaluate the proliferation and differentiation potential of stem cells from the apical papilla (SCAP) seeded along with platelet rich fibrin (PRF) scaffolds prepared under two different centrifugation protocols. Materials and Methods: Standard and advanc...
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| Format: | Article |
| Language: | English |
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Universidade Estadual Paulista
2023-12-01
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| Series: | Brazilian Dental Science |
| Online Access: | https://ojs.ict.unesp.br/index.php/cob/article/view/3847 |
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| author | Lina Shalaby Sahar Shawkat Iman Fathy |
| author_facet | Lina Shalaby Sahar Shawkat Iman Fathy |
| author_sort | Lina Shalaby |
| collection | DOAJ |
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Objectives: The present work was designed to evaluate the proliferation and differentiation potential of stem cells from the apical papilla (SCAP) seeded along with platelet rich fibrin (PRF) scaffolds prepared under two different centrifugation protocols. Materials and Methods: Standard and advanced PRF protocols were used. Cells were divided into 4 groups: negative control, positive control, standard (L-PRF) and advanced (A-PRF) groups. Cell count and cell viability assays were carried out to assess the proliferation capacity. Alizarin red S (ARS) stain, Alkaline phosphatase (ALP) activity and Receptor activator of nuclear factor-kappa B ligand (RANKL) immunofluorescence staining were used to evaluate the osteogenic potential in the differentiated cells. Results: Both types of platelet rich fibrin increased the cell count, cell viability with no cytotoxicity that was reflected on increased proliferation and differentiation in terms of the performed tests. Conclusion: A-PRF group showed significant increase in proliferation and differentiation potentials compared to L-PRF group.
KEYWORDS
Alkaline phosphatase; Centrifugation; Platelet rich fibrin; RANKL ligand; Stem cells.
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| format | Article |
| id | doaj-art-d00d39d2a4a446e5847a026faade8d65 |
| institution | Kabale University |
| issn | 2178-6011 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Universidade Estadual Paulista |
| record_format | Article |
| series | Brazilian Dental Science |
| spelling | doaj-art-d00d39d2a4a446e5847a026faade8d652025-08-22T14:53:02ZengUniversidade Estadual PaulistaBrazilian Dental Science2178-60112023-12-0126410.4322/bds.2023.e3847Potential effect of platelet rich fibrin prepared under different centrifugation protocols on stem cells from the apical papillaLina Shalaby0https://orcid.org/0000-0001-8894-0993Sahar Shawkat1https://orcid.org/0000-0001-9322-1715Iman Fathy2https://orcid.org/0000-0002-5219-2740Newgiza University, School of Dentistry, Oral Biology Department, Giza, Egypt.Cairo University, Faculty of Dentistry, Oral Biology Department, Cairo, Egypt.Ain Shams University, Faculty of Dentistry, Oral Biology Department, Cairo, Egypt. Objectives: The present work was designed to evaluate the proliferation and differentiation potential of stem cells from the apical papilla (SCAP) seeded along with platelet rich fibrin (PRF) scaffolds prepared under two different centrifugation protocols. Materials and Methods: Standard and advanced PRF protocols were used. Cells were divided into 4 groups: negative control, positive control, standard (L-PRF) and advanced (A-PRF) groups. Cell count and cell viability assays were carried out to assess the proliferation capacity. Alizarin red S (ARS) stain, Alkaline phosphatase (ALP) activity and Receptor activator of nuclear factor-kappa B ligand (RANKL) immunofluorescence staining were used to evaluate the osteogenic potential in the differentiated cells. Results: Both types of platelet rich fibrin increased the cell count, cell viability with no cytotoxicity that was reflected on increased proliferation and differentiation in terms of the performed tests. Conclusion: A-PRF group showed significant increase in proliferation and differentiation potentials compared to L-PRF group. KEYWORDS Alkaline phosphatase; Centrifugation; Platelet rich fibrin; RANKL ligand; Stem cells. https://ojs.ict.unesp.br/index.php/cob/article/view/3847 |
| spellingShingle | Lina Shalaby Sahar Shawkat Iman Fathy Potential effect of platelet rich fibrin prepared under different centrifugation protocols on stem cells from the apical papilla Brazilian Dental Science |
| title | Potential effect of platelet rich fibrin prepared under different centrifugation protocols on stem cells from the apical papilla |
| title_full | Potential effect of platelet rich fibrin prepared under different centrifugation protocols on stem cells from the apical papilla |
| title_fullStr | Potential effect of platelet rich fibrin prepared under different centrifugation protocols on stem cells from the apical papilla |
| title_full_unstemmed | Potential effect of platelet rich fibrin prepared under different centrifugation protocols on stem cells from the apical papilla |
| title_short | Potential effect of platelet rich fibrin prepared under different centrifugation protocols on stem cells from the apical papilla |
| title_sort | potential effect of platelet rich fibrin prepared under different centrifugation protocols on stem cells from the apical papilla |
| url | https://ojs.ict.unesp.br/index.php/cob/article/view/3847 |
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