Cloning and Transcriptional Activity Analysis of the Bovine <i>CDH11</i> Gene Promoter: Transcription Factors Sp1 and GR Regulate Bovine <i>CDH11</i> Expression
Growth rate of bovine skeletal muscle has a major impact on beef yield. Cadherin-11 (<i>CDH11</i>) was found to be a potential candidate gene for growth and development in beef cattle. This study confirmed the high expression of <i>CDH11</i> in bovine <i>longissimus dor...
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2025-04-01
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| author | Zhanxin Liu Yanbin Bai Zongchang Chen Yanmei Niu Xue Jia Liang Li Xiaolan Zhang Binggang Shi Zhidong Zhao Jiang Hu Yuzhu Luo Jiqing Wang Xiu Liu Shaobin Li Fangfang Zhao |
| author_facet | Zhanxin Liu Yanbin Bai Zongchang Chen Yanmei Niu Xue Jia Liang Li Xiaolan Zhang Binggang Shi Zhidong Zhao Jiang Hu Yuzhu Luo Jiqing Wang Xiu Liu Shaobin Li Fangfang Zhao |
| author_sort | Zhanxin Liu |
| collection | DOAJ |
| description | Growth rate of bovine skeletal muscle has a major impact on beef yield. Cadherin-11 (<i>CDH11</i>) was found to be a potential candidate gene for growth and development in beef cattle. This study confirmed the high expression of <i>CDH11</i> in bovine <i>longissimus dorsi</i> using a tissue expression analysis. To understand the transcriptional regulation mechanism of <i>CDH11</i> gene, we constructed a double luciferase vector for the promoter region of <i>CDH11</i> gene and determined that the core transcriptional regulatory region was located at −129/+55 bp relative to the transcription start site (TSS). In addition, we confirmed that skeletal muscle growth and development-related transcription factor-specific protein 1 (SP1) and glucocorticoid receptor (GR) bind to the <i>CDH11</i> gene promoter region at −36/−27 bp and −20/−11 bp, respectively, to regulate <i>CDH11</i> expression. These interactions provide valuable information for understanding the mechanism of transcriptional regulation of the bovine <i>CDH11</i> gene during muscle growth and development. |
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| institution | DOAJ |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-04-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj-art-d00abaf0b75f40898ff0fa7f82f451592025-08-20T02:59:14ZengMDPI AGAnimals2076-26152025-04-01159121710.3390/ani15091217Cloning and Transcriptional Activity Analysis of the Bovine <i>CDH11</i> Gene Promoter: Transcription Factors Sp1 and GR Regulate Bovine <i>CDH11</i> ExpressionZhanxin Liu0Yanbin Bai1Zongchang Chen2Yanmei Niu3Xue Jia4Liang Li5Xiaolan Zhang6Binggang Shi7Zhidong Zhao8Jiang Hu9Yuzhu Luo10Jiqing Wang11Xiu Liu12Shaobin Li13Fangfang Zhao14Gansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGrowth rate of bovine skeletal muscle has a major impact on beef yield. Cadherin-11 (<i>CDH11</i>) was found to be a potential candidate gene for growth and development in beef cattle. This study confirmed the high expression of <i>CDH11</i> in bovine <i>longissimus dorsi</i> using a tissue expression analysis. To understand the transcriptional regulation mechanism of <i>CDH11</i> gene, we constructed a double luciferase vector for the promoter region of <i>CDH11</i> gene and determined that the core transcriptional regulatory region was located at −129/+55 bp relative to the transcription start site (TSS). In addition, we confirmed that skeletal muscle growth and development-related transcription factor-specific protein 1 (SP1) and glucocorticoid receptor (GR) bind to the <i>CDH11</i> gene promoter region at −36/−27 bp and −20/−11 bp, respectively, to regulate <i>CDH11</i> expression. These interactions provide valuable information for understanding the mechanism of transcriptional regulation of the bovine <i>CDH11</i> gene during muscle growth and development.https://www.mdpi.com/2076-2615/15/9/1217bovine<i>CDH11</i> genetranscriptional regulationskeletal muscle |
| spellingShingle | Zhanxin Liu Yanbin Bai Zongchang Chen Yanmei Niu Xue Jia Liang Li Xiaolan Zhang Binggang Shi Zhidong Zhao Jiang Hu Yuzhu Luo Jiqing Wang Xiu Liu Shaobin Li Fangfang Zhao Cloning and Transcriptional Activity Analysis of the Bovine <i>CDH11</i> Gene Promoter: Transcription Factors Sp1 and GR Regulate Bovine <i>CDH11</i> Expression Animals bovine <i>CDH11</i> gene transcriptional regulation skeletal muscle |
| title | Cloning and Transcriptional Activity Analysis of the Bovine <i>CDH11</i> Gene Promoter: Transcription Factors Sp1 and GR Regulate Bovine <i>CDH11</i> Expression |
| title_full | Cloning and Transcriptional Activity Analysis of the Bovine <i>CDH11</i> Gene Promoter: Transcription Factors Sp1 and GR Regulate Bovine <i>CDH11</i> Expression |
| title_fullStr | Cloning and Transcriptional Activity Analysis of the Bovine <i>CDH11</i> Gene Promoter: Transcription Factors Sp1 and GR Regulate Bovine <i>CDH11</i> Expression |
| title_full_unstemmed | Cloning and Transcriptional Activity Analysis of the Bovine <i>CDH11</i> Gene Promoter: Transcription Factors Sp1 and GR Regulate Bovine <i>CDH11</i> Expression |
| title_short | Cloning and Transcriptional Activity Analysis of the Bovine <i>CDH11</i> Gene Promoter: Transcription Factors Sp1 and GR Regulate Bovine <i>CDH11</i> Expression |
| title_sort | cloning and transcriptional activity analysis of the bovine i cdh11 i gene promoter transcription factors sp1 and gr regulate bovine i cdh11 i expression |
| topic | bovine <i>CDH11</i> gene transcriptional regulation skeletal muscle |
| url | https://www.mdpi.com/2076-2615/15/9/1217 |
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