Transovarial transmission of Rickettsia spp., Francisella-like endosymbionts, and Spiroplasma spp. in Dermacentor reticulatus ticks
Research on the transovarial transmission of pathogens whose reservoirs and vectors are ticks has led to an understanding of the mechanisms related to the circulation and persistence of selected microorganisms in natural foci. The primary aim of this study was to investigate the possibility of trans...
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Elsevier
2024-11-01
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| Series: | Ticks and Tick-Borne Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1877959X24001146 |
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| author | Anna Kloc Angelina Wójcik-Fatla Piotr Paprzycki Lech Panasiuk |
| author_facet | Anna Kloc Angelina Wójcik-Fatla Piotr Paprzycki Lech Panasiuk |
| author_sort | Anna Kloc |
| collection | DOAJ |
| description | Research on the transovarial transmission of pathogens whose reservoirs and vectors are ticks has led to an understanding of the mechanisms related to the circulation and persistence of selected microorganisms in natural foci. The primary aim of this study was to investigate the possibility of transovarial transmission of Rickettsia spp. in Dermacentor reticulatus ticks, and the influence of Francisella-like endosymbionts (FLEs) and Spiroplasma spp. on the efficiency of the egg-laying process and transmission of selected pathogens.In total, 16,600 eggs were obtained under laboratory conditions from 55 females, with an average of 346 eggs per female. Adults, eggs, and hatched larvae were tested using polymerase chain reaction (PCR) for the presence of Rickettsia and endosymbionts.DNA fragments of Rickettsia spp. were found in females (56.4 %) and in pools of eggs (72.9 %) and larvae (62.4 %). FLEs and Spiroplasma endosymbionts were confirmed in females (80 % and 14.5 %, respectively), pools of eggs (81.6 % and 26.1 %, respectively), and larvae (82.7 % and 46.2 %, respectively). Transovarial transmission was confirmed in Rickettsia raoultii, FLEs, and Spiroplasma ixodetis. No correlation was observed between the occurrence of individual endosymbionts and the efficiency of egg laying and transovarial transmission in Rickettsia spp.In conclusion, transovarial transmission of Rickettsia spp., FLEs and Spiroplasma spp. in D. reticulatus plays an important role in their persistence and circulation in the environment. However, further research is required on this topic. |
| format | Article |
| id | doaj-art-d009b5c8ea01422984f53658f49c4dc2 |
| institution | OA Journals |
| issn | 1877-9603 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ticks and Tick-Borne Diseases |
| spelling | doaj-art-d009b5c8ea01422984f53658f49c4dc22025-08-20T02:39:08ZengElsevierTicks and Tick-Borne Diseases1877-96032024-11-0115610242110.1016/j.ttbdis.2024.102421Transovarial transmission of Rickettsia spp., Francisella-like endosymbionts, and Spiroplasma spp. in Dermacentor reticulatus ticksAnna Kloc0Angelina Wójcik-Fatla1Piotr Paprzycki2Lech Panasiuk3Department of Health Biohazards and Parasitology, Institute of Rural Health, Lublin, PolandDepartment of Health Biohazards and Parasitology, Institute of Rural Health, Lublin, Poland; Corresponding author at: Institute of Rural Health, Jaczewskiego 2, 20-090 Lublin, Poland.Department of Medical Diagnostic Research, Institute of Rural Health, Lublin, PolandClinic of Internal Medicine with the Occupational Diseases and the Diagnostics Subunit, Institute of Rural Health, Lublin, PolandResearch on the transovarial transmission of pathogens whose reservoirs and vectors are ticks has led to an understanding of the mechanisms related to the circulation and persistence of selected microorganisms in natural foci. The primary aim of this study was to investigate the possibility of transovarial transmission of Rickettsia spp. in Dermacentor reticulatus ticks, and the influence of Francisella-like endosymbionts (FLEs) and Spiroplasma spp. on the efficiency of the egg-laying process and transmission of selected pathogens.In total, 16,600 eggs were obtained under laboratory conditions from 55 females, with an average of 346 eggs per female. Adults, eggs, and hatched larvae were tested using polymerase chain reaction (PCR) for the presence of Rickettsia and endosymbionts.DNA fragments of Rickettsia spp. were found in females (56.4 %) and in pools of eggs (72.9 %) and larvae (62.4 %). FLEs and Spiroplasma endosymbionts were confirmed in females (80 % and 14.5 %, respectively), pools of eggs (81.6 % and 26.1 %, respectively), and larvae (82.7 % and 46.2 %, respectively). Transovarial transmission was confirmed in Rickettsia raoultii, FLEs, and Spiroplasma ixodetis. No correlation was observed between the occurrence of individual endosymbionts and the efficiency of egg laying and transovarial transmission in Rickettsia spp.In conclusion, transovarial transmission of Rickettsia spp., FLEs and Spiroplasma spp. in D. reticulatus plays an important role in their persistence and circulation in the environment. However, further research is required on this topic.http://www.sciencedirect.com/science/article/pii/S1877959X24001146Transovarial transmissionDermacentor reticulatusRickettsiaSpiroplasmaFrancisella-like endosymbiont |
| spellingShingle | Anna Kloc Angelina Wójcik-Fatla Piotr Paprzycki Lech Panasiuk Transovarial transmission of Rickettsia spp., Francisella-like endosymbionts, and Spiroplasma spp. in Dermacentor reticulatus ticks Ticks and Tick-Borne Diseases Transovarial transmission Dermacentor reticulatus Rickettsia Spiroplasma Francisella-like endosymbiont |
| title | Transovarial transmission of Rickettsia spp., Francisella-like endosymbionts, and Spiroplasma spp. in Dermacentor reticulatus ticks |
| title_full | Transovarial transmission of Rickettsia spp., Francisella-like endosymbionts, and Spiroplasma spp. in Dermacentor reticulatus ticks |
| title_fullStr | Transovarial transmission of Rickettsia spp., Francisella-like endosymbionts, and Spiroplasma spp. in Dermacentor reticulatus ticks |
| title_full_unstemmed | Transovarial transmission of Rickettsia spp., Francisella-like endosymbionts, and Spiroplasma spp. in Dermacentor reticulatus ticks |
| title_short | Transovarial transmission of Rickettsia spp., Francisella-like endosymbionts, and Spiroplasma spp. in Dermacentor reticulatus ticks |
| title_sort | transovarial transmission of rickettsia spp francisella like endosymbionts and spiroplasma spp in dermacentor reticulatus ticks |
| topic | Transovarial transmission Dermacentor reticulatus Rickettsia Spiroplasma Francisella-like endosymbiont |
| url | http://www.sciencedirect.com/science/article/pii/S1877959X24001146 |
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