Ga/Lu-labeled DOTA-TATE shows similar imaging and biodistribution in neuroendocrine tumor model
Somatostatin receptors are overexpressed in neuroendocrine tumors, whose endogenous ligands are somatostatin. DOTA-TATE is an analogue of somatostatin, which shows high binding affinity to somatostatin receptors. We aim to evaluate the 68 Ga/ 177 Lu-labeling DOTA-TATE kit in neuroendocrine tumor mod...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-05-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317705519 |
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| author | Fei Liu Hua Zhu Jiangyuan Yu Xuedi Han Qinghua Xie Teli Liu Chuanqin Xia Nan Li Zhi Yang |
| author_facet | Fei Liu Hua Zhu Jiangyuan Yu Xuedi Han Qinghua Xie Teli Liu Chuanqin Xia Nan Li Zhi Yang |
| author_sort | Fei Liu |
| collection | DOAJ |
| description | Somatostatin receptors are overexpressed in neuroendocrine tumors, whose endogenous ligands are somatostatin. DOTA-TATE is an analogue of somatostatin, which shows high binding affinity to somatostatin receptors. We aim to evaluate the 68 Ga/ 177 Lu-labeling DOTA-TATE kit in neuroendocrine tumor model for molecular imaging and to try human–positron emission tomography/computed tomography imaging of 68 Ga-DOTA-TATE in neuroendocrine tumor patients. DOTA-TATE kits were formulated and radiolabeled with 68 Ga/ 177 Lu for 68 Ga/ 177 Lu-DOTA-TATE (M-DOTA-TATE). In vitro and in vivo stability of 177 Lu-DOTA-TATE were performed. Nude mice bearing human tumors were injected with 68 Ga-DOTA-TATE or 177 Lu-DOTA-TATE for micro–positron emission tomography and micro-single-photon emission computed tomography/computed tomography imaging separately, and clinical positron emission tomography/computed tomography images of 68 Ga-DOTA-TATE were obtained at 1 h post-intravenous injection from patients with neuroendocrine tumors. Micro–positron emission tomography and micro-single-photon emission computed tomography/computed tomography imaging of 68 Ga-DOTA-TATE and 177 Lu-DOTA-TATE both showed clear tumor uptake which could be blocked by excess DOTA-TATE. In addition, 68 Ga-DOTA-TATE–positron emission tomography/computed tomography imaging in neuroendocrine tumor patients could show primary and metastatic lesions. 68 Ga-DOTA-TATE and 177 Lu-DOTA-TATE could accumulate in tumors in animal models, paving the way for better clinical peptide receptor radionuclide therapy for neuroendocrine tumor patients in Asian population. |
| format | Article |
| id | doaj-art-d008df44a09044dfbe99c70c730c17cb |
| institution | Kabale University |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-d008df44a09044dfbe99c70c730c17cb2025-08-20T03:39:15ZengSAGE PublishingTumor Biology1423-03802017-05-013910.1177/1010428317705519Ga/Lu-labeled DOTA-TATE shows similar imaging and biodistribution in neuroendocrine tumor modelFei Liu0Hua Zhu1Jiangyuan Yu2Xuedi Han3Qinghua Xie4Teli Liu5Chuanqin Xia6Nan Li7Zhi Yang8Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing, ChinaCollege of Chemistry, Sichuan University, Chengdu, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing, ChinaCollege of Chemistry, Sichuan University, Chengdu, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing, ChinaSomatostatin receptors are overexpressed in neuroendocrine tumors, whose endogenous ligands are somatostatin. DOTA-TATE is an analogue of somatostatin, which shows high binding affinity to somatostatin receptors. We aim to evaluate the 68 Ga/ 177 Lu-labeling DOTA-TATE kit in neuroendocrine tumor model for molecular imaging and to try human–positron emission tomography/computed tomography imaging of 68 Ga-DOTA-TATE in neuroendocrine tumor patients. DOTA-TATE kits were formulated and radiolabeled with 68 Ga/ 177 Lu for 68 Ga/ 177 Lu-DOTA-TATE (M-DOTA-TATE). In vitro and in vivo stability of 177 Lu-DOTA-TATE were performed. Nude mice bearing human tumors were injected with 68 Ga-DOTA-TATE or 177 Lu-DOTA-TATE for micro–positron emission tomography and micro-single-photon emission computed tomography/computed tomography imaging separately, and clinical positron emission tomography/computed tomography images of 68 Ga-DOTA-TATE were obtained at 1 h post-intravenous injection from patients with neuroendocrine tumors. Micro–positron emission tomography and micro-single-photon emission computed tomography/computed tomography imaging of 68 Ga-DOTA-TATE and 177 Lu-DOTA-TATE both showed clear tumor uptake which could be blocked by excess DOTA-TATE. In addition, 68 Ga-DOTA-TATE–positron emission tomography/computed tomography imaging in neuroendocrine tumor patients could show primary and metastatic lesions. 68 Ga-DOTA-TATE and 177 Lu-DOTA-TATE could accumulate in tumors in animal models, paving the way for better clinical peptide receptor radionuclide therapy for neuroendocrine tumor patients in Asian population.https://doi.org/10.1177/1010428317705519 |
| spellingShingle | Fei Liu Hua Zhu Jiangyuan Yu Xuedi Han Qinghua Xie Teli Liu Chuanqin Xia Nan Li Zhi Yang Ga/Lu-labeled DOTA-TATE shows similar imaging and biodistribution in neuroendocrine tumor model Tumor Biology |
| title | Ga/Lu-labeled DOTA-TATE shows similar imaging and biodistribution in neuroendocrine tumor model |
| title_full | Ga/Lu-labeled DOTA-TATE shows similar imaging and biodistribution in neuroendocrine tumor model |
| title_fullStr | Ga/Lu-labeled DOTA-TATE shows similar imaging and biodistribution in neuroendocrine tumor model |
| title_full_unstemmed | Ga/Lu-labeled DOTA-TATE shows similar imaging and biodistribution in neuroendocrine tumor model |
| title_short | Ga/Lu-labeled DOTA-TATE shows similar imaging and biodistribution in neuroendocrine tumor model |
| title_sort | ga lu labeled dota tate shows similar imaging and biodistribution in neuroendocrine tumor model |
| url | https://doi.org/10.1177/1010428317705519 |
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