Application and analysis of compatible platelets matching via antigen avoidance
[Objective] To investigate the application of antigen avoidance pattern for compatible platelets matching. [Methods] Samples from two patients with immune-mediated platelet transfusion refractoriness were screened for platelet antibodies using solid-phase red blood cell adhesion assay (SPRCA). The g...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Institute of Blood Transfusion of Chinese Academy of Medical Sciences
2025-06-01
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| Series: | Zhongguo shuxue zazhi |
| Subjects: | |
| Online Access: | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.06.015&lang=en |
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| Summary: | [Objective] To investigate the application of antigen avoidance pattern for compatible platelets matching. [Methods] Samples from two patients with immune-mediated platelet transfusion refractoriness were screened for platelet antibodies using solid-phase red blood cell adhesion assay (SPRCA). The genotypes of HLA-A, -B loci were determined via ploymerase chain reaction sequence. The specificity of HLA class I antibodies was detected using Luminex technology. [Results] Platelet antibody screening via SPRCA yielded positive results in both samples. Antibody specificity testing showed the presence of antibodies against HLA-B65, A80, B13, as well as antibodies against HLA-A11, B52, A24 respectively, with both patients exhibiting 80 kinds of positive antibodies. The antibody avoidance pattern successfully selected compatible platelets for transfusion. The bleeding symptoms of two patients were improved after compatible platelets transfusion. [Conclusion] For blood stations with limited platelet gene bank resources, antibody avoidance pattern for compatible platelets matching represents an effective strategy for immune-mediated platelet transfusion refractoriness. |
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| ISSN: | 1004-549X |