Galbanic acid alleviates lead acetate-induced reproductive toxicity in prepubertal male wistar rats
Abstract Lead (Pb) is an environmental toxicant reported to impair male reproductive system. Galbanic acid (GBA), a natural sesquiterpene coumarin compound renowned for its diverse biological activities, exhibits anti-apoptotic, anti-hypoxic, anti-proliferative, anti-hepatitis, anti-angiogenic, anti...
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Nature Portfolio
2025-07-01
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| author | Nasrin Gharibshahi Hanaa Salem Saleh Gazwi Milad Khosravi Somayeh Khosravi-Farsani Farnaz Khadivi Mohammad Hadi Zarei |
| author_facet | Nasrin Gharibshahi Hanaa Salem Saleh Gazwi Milad Khosravi Somayeh Khosravi-Farsani Farnaz Khadivi Mohammad Hadi Zarei |
| author_sort | Nasrin Gharibshahi |
| collection | DOAJ |
| description | Abstract Lead (Pb) is an environmental toxicant reported to impair male reproductive system. Galbanic acid (GBA), a natural sesquiterpene coumarin compound renowned for its diverse biological activities, exhibits anti-apoptotic, anti-hypoxic, anti-proliferative, anti-hepatitis, anti-angiogenic, antibacterial, and anti-thrombotic effects. In this experimental study, we investigated the protective effects of GBA on lead acetate-induced testicular damage in rats. Thirty-two immature male rats were divided equally into four groups: control group, PbAc group (0.3% lead acetate in drinking water), GBA group (1 mg/kg daily) and PbAc + GBA group (0.3% lead acetate in drinking water + 1 mg/kg daily GBA). Our results indicated that the lead-administration significantly reduced the mean number of different cells at seminiferous tubules, increase the ROS and MDA levels and also cause histopathological changes in testis (p < 0.001). Galbanic acid inhibited the reduction in number of cells, generation of ROS and MDA and histophatological changes in PbAc + GBA group (p < 0.001). In addition, Bax/Bcl-2 ratio expression which was up-regulated in PbAc group, downregulated by co-administration of Galbanic acid and lead in in comparison with PbAc group (p < 0.001). In conclusion, PbAc administration adversely impacted on the testis; on the other hand, Galbanic acid co-administration with lead partially ameliorated these adverse effects. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-07-01 |
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| spelling | doaj-art-d0003bf7efaa4b87802ac8ffee4ed5442025-08-20T04:01:34ZengNature PortfolioScientific Reports2045-23222025-07-0115111110.1038/s41598-025-06661-yGalbanic acid alleviates lead acetate-induced reproductive toxicity in prepubertal male wistar ratsNasrin Gharibshahi0Hanaa Salem Saleh Gazwi1Milad Khosravi2Somayeh Khosravi-Farsani3Farnaz Khadivi4Mohammad Hadi Zarei5Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical SciencesDepartment of Agricultural Chemistry, Faculty of Agriculture, Minia UniversityStudent Research Committee, Shahrekord University of Medical SciencesDepartment of Anatomy, School of Medicine, Shahrekord University of Medical SciencesDepartment of Anatomy, School of Medicine, Shahrekord University of Medical SciencesMedical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical SciencesAbstract Lead (Pb) is an environmental toxicant reported to impair male reproductive system. Galbanic acid (GBA), a natural sesquiterpene coumarin compound renowned for its diverse biological activities, exhibits anti-apoptotic, anti-hypoxic, anti-proliferative, anti-hepatitis, anti-angiogenic, antibacterial, and anti-thrombotic effects. In this experimental study, we investigated the protective effects of GBA on lead acetate-induced testicular damage in rats. Thirty-two immature male rats were divided equally into four groups: control group, PbAc group (0.3% lead acetate in drinking water), GBA group (1 mg/kg daily) and PbAc + GBA group (0.3% lead acetate in drinking water + 1 mg/kg daily GBA). Our results indicated that the lead-administration significantly reduced the mean number of different cells at seminiferous tubules, increase the ROS and MDA levels and also cause histopathological changes in testis (p < 0.001). Galbanic acid inhibited the reduction in number of cells, generation of ROS and MDA and histophatological changes in PbAc + GBA group (p < 0.001). In addition, Bax/Bcl-2 ratio expression which was up-regulated in PbAc group, downregulated by co-administration of Galbanic acid and lead in in comparison with PbAc group (p < 0.001). In conclusion, PbAc administration adversely impacted on the testis; on the other hand, Galbanic acid co-administration with lead partially ameliorated these adverse effects.https://doi.org/10.1038/s41598-025-06661-yLead acetateGalbanic acidMale reproductive toxicity |
| spellingShingle | Nasrin Gharibshahi Hanaa Salem Saleh Gazwi Milad Khosravi Somayeh Khosravi-Farsani Farnaz Khadivi Mohammad Hadi Zarei Galbanic acid alleviates lead acetate-induced reproductive toxicity in prepubertal male wistar rats Scientific Reports Lead acetate Galbanic acid Male reproductive toxicity |
| title | Galbanic acid alleviates lead acetate-induced reproductive toxicity in prepubertal male wistar rats |
| title_full | Galbanic acid alleviates lead acetate-induced reproductive toxicity in prepubertal male wistar rats |
| title_fullStr | Galbanic acid alleviates lead acetate-induced reproductive toxicity in prepubertal male wistar rats |
| title_full_unstemmed | Galbanic acid alleviates lead acetate-induced reproductive toxicity in prepubertal male wistar rats |
| title_short | Galbanic acid alleviates lead acetate-induced reproductive toxicity in prepubertal male wistar rats |
| title_sort | galbanic acid alleviates lead acetate induced reproductive toxicity in prepubertal male wistar rats |
| topic | Lead acetate Galbanic acid Male reproductive toxicity |
| url | https://doi.org/10.1038/s41598-025-06661-y |
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