Effect of organic amendments on the decontamination potential of heavy metals by Staphylococcus aureus and Bacillus cereus in soil contaminated with spent engine oil
This study was conducted to assess the decontamination of heavy metals of an environmental concern in spent engine oil (SEO) contaminated soil, through the adoption of Staphylococcus aureus and Bacillus cereus cocultures, isolated from Dutse mechanic village, Nigeria. About 1.5 kg of autoclaved soi...
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| Format: | Article |
| Language: | English |
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ResearchersLinks, Ltd
2019-10-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_54352_bdffaa94a1abcc8943bb48be08093d6d.pdf |
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| author | Adeniyi O. Adeleye Mohammed B. Yerima Michael E. Nkereuwem Victor O. Onokebhagbe Peter G. Shiaka Florence K. Amoo Ibrahim K. Adam |
| author_facet | Adeniyi O. Adeleye Mohammed B. Yerima Michael E. Nkereuwem Victor O. Onokebhagbe Peter G. Shiaka Florence K. Amoo Ibrahim K. Adam |
| author_sort | Adeniyi O. Adeleye |
| collection | DOAJ |
| description | This study was conducted to assess the decontamination of heavy metals of an environmental concern in spent
engine oil (SEO) contaminated soil, through the adoption of Staphylococcus aureus and Bacillus cereus cocultures, isolated from Dutse mechanic village, Nigeria. About 1.5 kg of autoclaved soil was contaminated with
SEO at three levels. The sterilized soil was then amended with compost, powdered cocoa pod husk (CPH), and
powdered cow dung (CD). Subsequently, bio-augmentation with bacterial co-cultures (150 ml) was done. Heavy
metal concentrations; Arsenic (As), Cadmium (Cd), Chromium (Cr), Nickel (Ni) and Lead (Pb) were estimated at
the commencement, at the fifth and tenth week of the study. The factorial experiment was laid out in a completely
randomized design (CRD). Results indicate that bio-stimulation adopted through the amendments did not have
significant effects on the bacterial decontamination of soil especially at the 5th week (P> 0.05). CD only influenced
the decontamination of Cd at 5% contamination level (0.0008 mg\ kg), compared with compost that recorded the
least effect (0.00360 mg\ kg) at the 5th week. Meanwhile, CPH had the highest decontamination effect on Cr (0.004
and 0.000 mg\ kg) at 10% and 15% contamination levels; respectively, at the 5th week. However at the 10th week,
complete removal of As was influenced by compost, CPH, and CD using 5%, 10% and 15% SEO contamination
levels, respectively (P< 0.05). The decontamination efficacies of S. aureus and B. cereus recorded in this study is
an indication of their potentialities for application in bioremediation of heavy metals. |
| format | Article |
| id | doaj-art-d0000bb89cd949b0a45efd2ec2fda616 |
| institution | Kabale University |
| issn | 2537-0286 2537-0294 |
| language | English |
| publishDate | 2019-10-01 |
| publisher | ResearchersLinks, Ltd |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj-art-d0000bb89cd949b0a45efd2ec2fda6162025-08-20T03:24:55ZengResearchersLinks, LtdNovel Research in Microbiology Journal2537-02862537-02942019-10-013547148410.21608/nrmj.2019.54352Effect of organic amendments on the decontamination potential of heavy metals by Staphylococcus aureus and Bacillus cereus in soil contaminated with spent engine oilAdeniyi O. Adeleye0Mohammed B. Yerima1Michael E. Nkereuwem2Victor O. Onokebhagbe3Peter G. Shiaka4Florence K. Amoo5Ibrahim K. Adam6Department of Environmental Sciences, Federal University Dutse, Dutse, Jigawa State, NigeriaDepartment of Microbiology and Biotechnology, Federal University Dutse, Dutse, Jigawa State, NigeriaDepartment of Soil Science, Federal University Dutse, Dutse, Jigawa State, NigeriaDepartment of Soil Science, Federal University Dutse, Dutse, Jigawa State, NigeriaDepartment of Microbiology and Biotechnology, Federal University Dutse, Dutse, Jigawa State, NigeriaDepartment of Microbiology and Biotechnology, Federal University Dutse, Dutse, Jigawa State, NigeriaDepartment of Biochemistry, Federal University Dutse, Dutse, Jigawa State, NigeriaThis study was conducted to assess the decontamination of heavy metals of an environmental concern in spent engine oil (SEO) contaminated soil, through the adoption of Staphylococcus aureus and Bacillus cereus cocultures, isolated from Dutse mechanic village, Nigeria. About 1.5 kg of autoclaved soil was contaminated with SEO at three levels. The sterilized soil was then amended with compost, powdered cocoa pod husk (CPH), and powdered cow dung (CD). Subsequently, bio-augmentation with bacterial co-cultures (150 ml) was done. Heavy metal concentrations; Arsenic (As), Cadmium (Cd), Chromium (Cr), Nickel (Ni) and Lead (Pb) were estimated at the commencement, at the fifth and tenth week of the study. The factorial experiment was laid out in a completely randomized design (CRD). Results indicate that bio-stimulation adopted through the amendments did not have significant effects on the bacterial decontamination of soil especially at the 5th week (P> 0.05). CD only influenced the decontamination of Cd at 5% contamination level (0.0008 mg\ kg), compared with compost that recorded the least effect (0.00360 mg\ kg) at the 5th week. Meanwhile, CPH had the highest decontamination effect on Cr (0.004 and 0.000 mg\ kg) at 10% and 15% contamination levels; respectively, at the 5th week. However at the 10th week, complete removal of As was influenced by compost, CPH, and CD using 5%, 10% and 15% SEO contamination levels, respectively (P< 0.05). The decontamination efficacies of S. aureus and B. cereus recorded in this study is an indication of their potentialities for application in bioremediation of heavy metals.https://nrmj.journals.ekb.eg/article_54352_bdffaa94a1abcc8943bb48be08093d6d.pdfsoilspent engine oilheavy metalsbacterial co-culturesbioremediationdecontamination |
| spellingShingle | Adeniyi O. Adeleye Mohammed B. Yerima Michael E. Nkereuwem Victor O. Onokebhagbe Peter G. Shiaka Florence K. Amoo Ibrahim K. Adam Effect of organic amendments on the decontamination potential of heavy metals by Staphylococcus aureus and Bacillus cereus in soil contaminated with spent engine oil Novel Research in Microbiology Journal soil spent engine oil heavy metals bacterial co-cultures bioremediation decontamination |
| title | Effect of organic amendments on the decontamination potential of heavy metals by Staphylococcus aureus and Bacillus cereus in soil contaminated with spent engine oil |
| title_full | Effect of organic amendments on the decontamination potential of heavy metals by Staphylococcus aureus and Bacillus cereus in soil contaminated with spent engine oil |
| title_fullStr | Effect of organic amendments on the decontamination potential of heavy metals by Staphylococcus aureus and Bacillus cereus in soil contaminated with spent engine oil |
| title_full_unstemmed | Effect of organic amendments on the decontamination potential of heavy metals by Staphylococcus aureus and Bacillus cereus in soil contaminated with spent engine oil |
| title_short | Effect of organic amendments on the decontamination potential of heavy metals by Staphylococcus aureus and Bacillus cereus in soil contaminated with spent engine oil |
| title_sort | effect of organic amendments on the decontamination potential of heavy metals by staphylococcus aureus and bacillus cereus in soil contaminated with spent engine oil |
| topic | soil spent engine oil heavy metals bacterial co-cultures bioremediation decontamination |
| url | https://nrmj.journals.ekb.eg/article_54352_bdffaa94a1abcc8943bb48be08093d6d.pdf |
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