Marginal adaptation and porosity of calcium silicate-based cements in furcation perforations: a micro-CT comparative study

Abstract This ex-vivo study compares the adaptation, porosity, and sealing performance of ProRoot MTA, NeoPutty, and Biodentine in standardized, simulated furcation perforations created in extracted mandibular molars using clinical evaluation and high-resolution micro-computed tomography (micro-CT)....

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Main Authors: María Rojo-Carpintero, Ana Martín-Díaz, Juan Miraglia Cantarini, Natalia Navarrete, Alejandro R. Pérez, Giulia Malvicini, José Aranguren, David Rubio-Flores
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-03729-7
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author María Rojo-Carpintero
Ana Martín-Díaz
Juan Miraglia Cantarini
Natalia Navarrete
Alejandro R. Pérez
Giulia Malvicini
José Aranguren
David Rubio-Flores
author_facet María Rojo-Carpintero
Ana Martín-Díaz
Juan Miraglia Cantarini
Natalia Navarrete
Alejandro R. Pérez
Giulia Malvicini
José Aranguren
David Rubio-Flores
author_sort María Rojo-Carpintero
collection DOAJ
description Abstract This ex-vivo study compares the adaptation, porosity, and sealing performance of ProRoot MTA, NeoPutty, and Biodentine in standardized, simulated furcation perforations created in extracted mandibular molars using clinical evaluation and high-resolution micro-computed tomography (micro-CT). Thirty-six mandibular molars with furcation perforations were randomly assigned to three groups (n = 12). Clinical evaluation assessed adaptation, porosity, and overfilling, while micro-computed tomography (micro-CT) provided quantitative data on voids and gaps. Statistical analysis used chi-square, Kruskal–Wallis, and Mann–Whitney U tests. NeoPutty and ProRoot MTA showed superior adaptation compared to Biodentine (p < 0.05). Biodentine showed higher porosity (28.44%) than ProRoot MTA (0%) and NeoPutty (8.3%) (p < 0.001). Biodentine also had the highest void volume (1.05 mm3) and gap volume (1.37 mm3), while ProRoot MTA recorded the lowest void volume (0.59 mm3), and NeoPutty had the smallest gap volume (0.85 mm3). No significant differences were observed in overfilling rates. Overall, ProRoot MTA exhibited the most consistent sealing ability, whereas NeoPutty emerged as a viable alternative due to its favorable handling and reliable adaptation. Biodentine, by contrast, showed the poorest performance in terms of structural integrity and sealing capacity. Within the limitations of this study, these findings support the use of ProRoot MTA or NeoPutty for furcation perforation repair, while suggesting more cautious use of Biodentine in such applications.
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spelling doaj-art-cffebe26cca6433583ef275cfda0b9582025-08-20T02:30:46ZengNature PortfolioScientific Reports2045-23222025-06-0115111010.1038/s41598-025-03729-7Marginal adaptation and porosity of calcium silicate-based cements in furcation perforations: a micro-CT comparative studyMaría Rojo-Carpintero0Ana Martín-Díaz1Juan Miraglia Cantarini2Natalia Navarrete3Alejandro R. Pérez4Giulia Malvicini5José Aranguren6David Rubio-Flores7Department of Endodontics, Rey Juan Carlos UniversityDepartment of Endodontics, Rey Juan Carlos UniversityPrivate Practice in Microscopic Endodontics and Apical MicrosurgeryDepartment of Endodontics, Rey Juan Carlos UniversityDepartment of Endodontics, Rey Juan Carlos UniversityDepartment of Medical Biotechnologies, Unit of Endodontics and Restorative Dentistry, University of SienaDepartment of Endodontics, Rey Juan Carlos UniversityDepartment of Endodontics, Rey Juan Carlos UniversityAbstract This ex-vivo study compares the adaptation, porosity, and sealing performance of ProRoot MTA, NeoPutty, and Biodentine in standardized, simulated furcation perforations created in extracted mandibular molars using clinical evaluation and high-resolution micro-computed tomography (micro-CT). Thirty-six mandibular molars with furcation perforations were randomly assigned to three groups (n = 12). Clinical evaluation assessed adaptation, porosity, and overfilling, while micro-computed tomography (micro-CT) provided quantitative data on voids and gaps. Statistical analysis used chi-square, Kruskal–Wallis, and Mann–Whitney U tests. NeoPutty and ProRoot MTA showed superior adaptation compared to Biodentine (p < 0.05). Biodentine showed higher porosity (28.44%) than ProRoot MTA (0%) and NeoPutty (8.3%) (p < 0.001). Biodentine also had the highest void volume (1.05 mm3) and gap volume (1.37 mm3), while ProRoot MTA recorded the lowest void volume (0.59 mm3), and NeoPutty had the smallest gap volume (0.85 mm3). No significant differences were observed in overfilling rates. Overall, ProRoot MTA exhibited the most consistent sealing ability, whereas NeoPutty emerged as a viable alternative due to its favorable handling and reliable adaptation. Biodentine, by contrast, showed the poorest performance in terms of structural integrity and sealing capacity. Within the limitations of this study, these findings support the use of ProRoot MTA or NeoPutty for furcation perforation repair, while suggesting more cautious use of Biodentine in such applications.https://doi.org/10.1038/s41598-025-03729-7Calcium silicate-based cementFurcal perforationsMandibular molarsMarginal adaptationMicro-computed tomographyPerforation sealing
spellingShingle María Rojo-Carpintero
Ana Martín-Díaz
Juan Miraglia Cantarini
Natalia Navarrete
Alejandro R. Pérez
Giulia Malvicini
José Aranguren
David Rubio-Flores
Marginal adaptation and porosity of calcium silicate-based cements in furcation perforations: a micro-CT comparative study
Scientific Reports
Calcium silicate-based cement
Furcal perforations
Mandibular molars
Marginal adaptation
Micro-computed tomography
Perforation sealing
title Marginal adaptation and porosity of calcium silicate-based cements in furcation perforations: a micro-CT comparative study
title_full Marginal adaptation and porosity of calcium silicate-based cements in furcation perforations: a micro-CT comparative study
title_fullStr Marginal adaptation and porosity of calcium silicate-based cements in furcation perforations: a micro-CT comparative study
title_full_unstemmed Marginal adaptation and porosity of calcium silicate-based cements in furcation perforations: a micro-CT comparative study
title_short Marginal adaptation and porosity of calcium silicate-based cements in furcation perforations: a micro-CT comparative study
title_sort marginal adaptation and porosity of calcium silicate based cements in furcation perforations a micro ct comparative study
topic Calcium silicate-based cement
Furcal perforations
Mandibular molars
Marginal adaptation
Micro-computed tomography
Perforation sealing
url https://doi.org/10.1038/s41598-025-03729-7
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