Dietary bile acids alleviate dextran sulfate sodium-induced enteritis in juvenile leopard coral grouper (Plectropomus leopardus)
Enteritis is a common disease in leopard coral grouper reared under intensive aquaculture conditions. We developed an enteritis model by feeding fish with 0.75 % dextran sulfate sodium (DSS) diet for one week, followed by a three-week period with diets containing graded bile acids levels (0 %, 0.15 ...
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Elsevier
2025-09-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425002625 |
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| author | Shengpeng Wang Longting Zhang Jie Huang Qingcong Fu Dingqian Guo Yujie Gao |
| author_facet | Shengpeng Wang Longting Zhang Jie Huang Qingcong Fu Dingqian Guo Yujie Gao |
| author_sort | Shengpeng Wang |
| collection | DOAJ |
| description | Enteritis is a common disease in leopard coral grouper reared under intensive aquaculture conditions. We developed an enteritis model by feeding fish with 0.75 % dextran sulfate sodium (DSS) diet for one week, followed by a three-week period with diets containing graded bile acids levels (0 %, 0.15 %, 0.30 %, 0.45 %, 0.60 %). They were designated as DSSBA0, DSSBA15, DSSBA30, DSSBA45, and DSSBA60, with an control group received no DSS treatment. The results showed that growth performance was significantly impaired in the DSSBA0 group compared with the control group (P < 0.05), while it improved markedly as the bile acids levels increased (P < 0.05). Intestinal indices were significantly enhanced in the dietary bile acids groups, particularly in the DSSBA60 group (P < 0.05). Meanwhile, bile acids supplementation ameliorated DSS induced intestinal damage, including villus atrophy and reduced muscular layer thickness. Moreover, the gene expression of tight junction proteins (zo1, zo2, zo3, occludin and claudin-1) were significantly increased in higher bile acids treatment groups compared with the DSSBA0 group (P < 0.05). Furthermore, gene expression analysis indicated that pro-inflammatory cytokines (tnf-α, inf-γ, il-1β, il-6, il-8) were down-regulated, while the anti-inflammatory cytokine il-10 was up-regulated in the higher bile acids groups (DSSBA45 and DSSBA60) compared with the DSSBA0 group (P < 0.05). And the activities of enzymes associated with intestinal inflammation, including myeloperoxidase (MPO) and β-N-acetyl-D-glucosaminidase (NAG), were significantly reduced in the groups supplemented with bile acids. Microbial diversity analysis through 16S rRNA sequencing indicated that dietary bile acids notably increased the abundance of Firmicutes and Candidatus-Arthromitus. The above findings provide strong evidence that bile acids effectively alleviate enteritis in leopard coral grouper by preserving intestinal integrity, modulating inflammatory responses, and enhanced beneficial bacteria abundance. These findings provide a novel nutritional strategy for improving gut health in aquaculture, offering valuable insights for sustainable fish farming. |
| format | Article |
| id | doaj-art-cffc7ed2c32148a3ab21e3c1d5c07ea8 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj-art-cffc7ed2c32148a3ab21e3c1d5c07ea82025-08-20T03:45:10ZengElsevierAquaculture Reports2352-51342025-09-014310287610.1016/j.aqrep.2025.102876Dietary bile acids alleviate dextran sulfate sodium-induced enteritis in juvenile leopard coral grouper (Plectropomus leopardus)Shengpeng Wang0Longting Zhang1Jie Huang2Qingcong Fu3Dingqian Guo4Yujie Gao5Dezhou Key Laboratory for Applied Bile Acid Research, Shandong Longchang Animal Health Product Co., Ltd., Dezhou, ChinaSchool of Marine Biology and Fisheries, Hainan University, Haikou, ChinaSchool of Marine Biology and Fisheries, Hainan University, Haikou, ChinaSchool of Marine Biology and Fisheries, Hainan University, Haikou, ChinaSchool of Tropical Agriculture and Forestry, Hainan University, Haikou, ChinaSchool of Marine Biology and Fisheries, Hainan University, Haikou, China; Correspondence to: School of Marine Biology and Fisheries, Hainan University, Haikou, Hainan 570228, China.Enteritis is a common disease in leopard coral grouper reared under intensive aquaculture conditions. We developed an enteritis model by feeding fish with 0.75 % dextran sulfate sodium (DSS) diet for one week, followed by a three-week period with diets containing graded bile acids levels (0 %, 0.15 %, 0.30 %, 0.45 %, 0.60 %). They were designated as DSSBA0, DSSBA15, DSSBA30, DSSBA45, and DSSBA60, with an control group received no DSS treatment. The results showed that growth performance was significantly impaired in the DSSBA0 group compared with the control group (P < 0.05), while it improved markedly as the bile acids levels increased (P < 0.05). Intestinal indices were significantly enhanced in the dietary bile acids groups, particularly in the DSSBA60 group (P < 0.05). Meanwhile, bile acids supplementation ameliorated DSS induced intestinal damage, including villus atrophy and reduced muscular layer thickness. Moreover, the gene expression of tight junction proteins (zo1, zo2, zo3, occludin and claudin-1) were significantly increased in higher bile acids treatment groups compared with the DSSBA0 group (P < 0.05). Furthermore, gene expression analysis indicated that pro-inflammatory cytokines (tnf-α, inf-γ, il-1β, il-6, il-8) were down-regulated, while the anti-inflammatory cytokine il-10 was up-regulated in the higher bile acids groups (DSSBA45 and DSSBA60) compared with the DSSBA0 group (P < 0.05). And the activities of enzymes associated with intestinal inflammation, including myeloperoxidase (MPO) and β-N-acetyl-D-glucosaminidase (NAG), were significantly reduced in the groups supplemented with bile acids. Microbial diversity analysis through 16S rRNA sequencing indicated that dietary bile acids notably increased the abundance of Firmicutes and Candidatus-Arthromitus. The above findings provide strong evidence that bile acids effectively alleviate enteritis in leopard coral grouper by preserving intestinal integrity, modulating inflammatory responses, and enhanced beneficial bacteria abundance. These findings provide a novel nutritional strategy for improving gut health in aquaculture, offering valuable insights for sustainable fish farming.http://www.sciencedirect.com/science/article/pii/S2352513425002625Plectropomus leopardusBile acidsIntestinal integrityInflammationMicrobiota |
| spellingShingle | Shengpeng Wang Longting Zhang Jie Huang Qingcong Fu Dingqian Guo Yujie Gao Dietary bile acids alleviate dextran sulfate sodium-induced enteritis in juvenile leopard coral grouper (Plectropomus leopardus) Aquaculture Reports Plectropomus leopardus Bile acids Intestinal integrity Inflammation Microbiota |
| title | Dietary bile acids alleviate dextran sulfate sodium-induced enteritis in juvenile leopard coral grouper (Plectropomus leopardus) |
| title_full | Dietary bile acids alleviate dextran sulfate sodium-induced enteritis in juvenile leopard coral grouper (Plectropomus leopardus) |
| title_fullStr | Dietary bile acids alleviate dextran sulfate sodium-induced enteritis in juvenile leopard coral grouper (Plectropomus leopardus) |
| title_full_unstemmed | Dietary bile acids alleviate dextran sulfate sodium-induced enteritis in juvenile leopard coral grouper (Plectropomus leopardus) |
| title_short | Dietary bile acids alleviate dextran sulfate sodium-induced enteritis in juvenile leopard coral grouper (Plectropomus leopardus) |
| title_sort | dietary bile acids alleviate dextran sulfate sodium induced enteritis in juvenile leopard coral grouper plectropomus leopardus |
| topic | Plectropomus leopardus Bile acids Intestinal integrity Inflammation Microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425002625 |
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