Molecular pharmacokinetic mechanism of quercetin-encapsulated polymeric micelles in alleviating cisplatin-induced nephrotoxicity and enhancing antineoplastic effects

IntroductionCisplatin (DDP), a platinum-based chemotherapy drug, shows broad antineoplastic activity, however, its clinical use is limited by dose-dependent nephrotoxicity, a major challenge in cancer therapy. The purpose of this study was to investigate the mechanism by which quercetin-polyethylene...

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Main Authors: Tangna Hao, Xiaokui Huo, Zhen Li, Changyuan Wang, Sha Wu, Anni Song, Fengyu Zhang, Kexin Liu
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Pharmacology
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Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2025.1590688/full
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author Tangna Hao
Tangna Hao
Tangna Hao
Xiaokui Huo
Zhen Li
Changyuan Wang
Changyuan Wang
Sha Wu
Anni Song
Fengyu Zhang
Kexin Liu
Kexin Liu
Kexin Liu
author_facet Tangna Hao
Tangna Hao
Tangna Hao
Xiaokui Huo
Zhen Li
Changyuan Wang
Changyuan Wang
Sha Wu
Anni Song
Fengyu Zhang
Kexin Liu
Kexin Liu
Kexin Liu
author_sort Tangna Hao
collection DOAJ
description IntroductionCisplatin (DDP), a platinum-based chemotherapy drug, shows broad antineoplastic activity, however, its clinical use is limited by dose-dependent nephrotoxicity, a major challenge in cancer therapy. The purpose of this study was to investigate the mechanism by which quercetin-polyethylene glycol-polycaprolactone (Que-PEG-PCL) micelles simultaneously enhance the cytotoxicity of DDP against cancer cells and reduce its nephrotoxicity.MethodsRodent models and HEK293 cells were used to evaluate the renoprotective effects of Que-PEG-PCL micelles. Pharmacokinetics focused on OCT2-mediated renal DDP disposition. Antitumor activity was assessed in CT26 cells and syngeneic tumors. Key assessments included oxidative stress, apoptosis, renal markers, and histopathology.Results:Que-PEG-PCL reduced DDP-induced nephrotoxicity, lowering creatinine and BUN to 42% and 38%. It also reduced oxidative stress and improved antioxidant activity. DDP plasma exposure increased to 323%, with renal clearance reduced to 14%, due to OCT2 inhibition. In a CT26 syngeneic model, combination therapy inhibited tumor volume by 84% compared to control group.DiscussionQue-PEG-PCL enhanced DDP’s therapeutic window by limiting renal accumulation and promoting tumor cell apoptosis. This dual-action strategy provides a novel approach for improving the clinical efficacy of DDP-based cancer therapy.
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spelling doaj-art-cffb62caf14743148cd3e0d3b46c15b02025-08-20T03:25:16ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-06-011610.3389/fphar.2025.15906881590688Molecular pharmacokinetic mechanism of quercetin-encapsulated polymeric micelles in alleviating cisplatin-induced nephrotoxicity and enhancing antineoplastic effectsTangna Hao0Tangna Hao1Tangna Hao2Xiaokui Huo3Zhen Li4Changyuan Wang5Changyuan Wang6Sha Wu7Anni Song8Fengyu Zhang9Kexin Liu10Kexin Liu11Kexin Liu12Institute of Integrative Medicine, Dalian Medical University, Dalian, ChinaProvincial Key Laboratory for Pharmacokinetics and Transport, Liaoning Dalian Medical University, Dalian, ChinaDepartment of Pharmacy, The Second Affiliated Hospital of Dalian Medical University, Dalian, ChinaPharmaceutical Research Center, Second Affiliated Hospital, Dalian Medical University, Dalian, ChinaCollege of Pharmacy, Dalian Medical University, Dalian, ChinaProvincial Key Laboratory for Pharmacokinetics and Transport, Liaoning Dalian Medical University, Dalian, ChinaDepartment of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian, ChinaDepartment of Pharmacy, The Second Affiliated Hospital of Dalian Medical University, Dalian, ChinaDepartment of Pharmacy, The Second Affiliated Hospital of Dalian Medical University, Dalian, ChinaDepartment of Pharmacy, The Second Affiliated Hospital of Dalian Medical University, Dalian, ChinaInstitute of Integrative Medicine, Dalian Medical University, Dalian, ChinaProvincial Key Laboratory for Pharmacokinetics and Transport, Liaoning Dalian Medical University, Dalian, ChinaDepartment of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian, ChinaIntroductionCisplatin (DDP), a platinum-based chemotherapy drug, shows broad antineoplastic activity, however, its clinical use is limited by dose-dependent nephrotoxicity, a major challenge in cancer therapy. The purpose of this study was to investigate the mechanism by which quercetin-polyethylene glycol-polycaprolactone (Que-PEG-PCL) micelles simultaneously enhance the cytotoxicity of DDP against cancer cells and reduce its nephrotoxicity.MethodsRodent models and HEK293 cells were used to evaluate the renoprotective effects of Que-PEG-PCL micelles. Pharmacokinetics focused on OCT2-mediated renal DDP disposition. Antitumor activity was assessed in CT26 cells and syngeneic tumors. Key assessments included oxidative stress, apoptosis, renal markers, and histopathology.Results:Que-PEG-PCL reduced DDP-induced nephrotoxicity, lowering creatinine and BUN to 42% and 38%. It also reduced oxidative stress and improved antioxidant activity. DDP plasma exposure increased to 323%, with renal clearance reduced to 14%, due to OCT2 inhibition. In a CT26 syngeneic model, combination therapy inhibited tumor volume by 84% compared to control group.DiscussionQue-PEG-PCL enhanced DDP’s therapeutic window by limiting renal accumulation and promoting tumor cell apoptosis. This dual-action strategy provides a novel approach for improving the clinical efficacy of DDP-based cancer therapy.https://www.frontiersin.org/articles/10.3389/fphar.2025.1590688/fullcisplatinnephrotoxicityquercetinpolymeric micellesantitumor potency
spellingShingle Tangna Hao
Tangna Hao
Tangna Hao
Xiaokui Huo
Zhen Li
Changyuan Wang
Changyuan Wang
Sha Wu
Anni Song
Fengyu Zhang
Kexin Liu
Kexin Liu
Kexin Liu
Molecular pharmacokinetic mechanism of quercetin-encapsulated polymeric micelles in alleviating cisplatin-induced nephrotoxicity and enhancing antineoplastic effects
Frontiers in Pharmacology
cisplatin
nephrotoxicity
quercetin
polymeric micelles
antitumor potency
title Molecular pharmacokinetic mechanism of quercetin-encapsulated polymeric micelles in alleviating cisplatin-induced nephrotoxicity and enhancing antineoplastic effects
title_full Molecular pharmacokinetic mechanism of quercetin-encapsulated polymeric micelles in alleviating cisplatin-induced nephrotoxicity and enhancing antineoplastic effects
title_fullStr Molecular pharmacokinetic mechanism of quercetin-encapsulated polymeric micelles in alleviating cisplatin-induced nephrotoxicity and enhancing antineoplastic effects
title_full_unstemmed Molecular pharmacokinetic mechanism of quercetin-encapsulated polymeric micelles in alleviating cisplatin-induced nephrotoxicity and enhancing antineoplastic effects
title_short Molecular pharmacokinetic mechanism of quercetin-encapsulated polymeric micelles in alleviating cisplatin-induced nephrotoxicity and enhancing antineoplastic effects
title_sort molecular pharmacokinetic mechanism of quercetin encapsulated polymeric micelles in alleviating cisplatin induced nephrotoxicity and enhancing antineoplastic effects
topic cisplatin
nephrotoxicity
quercetin
polymeric micelles
antitumor potency
url https://www.frontiersin.org/articles/10.3389/fphar.2025.1590688/full
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